Flow cytometric measurement of neutral red accumulation in earthworm coelomocytes: Novel assay for studies on heavy metal exposure

European Journal of Soil Biology

Volumn 43, Issue SUPPL. 1, 2007, Pages

  Plytycz, Barbara ^a   Klimek, Malgorzata ^a   Homa, Joanna ^a   Tylko, Grzegorz ^a   Kolaczkowska, Elzbieta ^a  

^a JAGIELLONIAN UNIVERSITY   (Poland)

Author keywords

Amoebocytes; Coelomocytes; Confocal microscopy; Eleocytes; Flow cytometry; Neutral red; Riboflavin

Indexed keywords

ALLOLOBOPHORA CHLOROTICA; DENDROBAENA VENETA; EISENIA FETIDA; INVERTEBRATA; LUMBRICUS SP.;

EID: 36248979299     PISSN: 11645563     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejsobi.2007.08.050     Document Type: Article

References (17)

1. Affar E.B., Dufour M., Poirier G.G., and Nadeau D. Isolation, purification and partial characterization of chloragocytes from the earthworm species Lumbricus terrestris. Mol. Cell. Biochem. 185 (1998) 123-133
2. Blanusa M., Varnai V.M., Piasek M., and Kostial K. Chelators as antidotes of metal toxicity: therapeutic and experimental aspects. Curr. Med. Chem. 12 (2005) 2771-2794
3. Cholewa J., Feeney G.P., O'Reilly M., Stürzenbaum S.R., Morgan A.J., and Plytycz B. Species specific autofluorescence in earthworm coelomocytes. Folia Histochem. Cytobiol. 44 (2006) 65-71
4. Cossarizza A., Pinti M., Troiano L., and Cooper E.L. Flow cytometry as a tool for analysing invertebrate cells. Invertebrate Survival J. 2 (2005) 32-40
5. Dailianis S., Domouhtsidou G.P., Raftopoulou E., Kaloyianni M., and Dimitriadis V.K. Evaluation of neutral red retention assay, micronucleus test, acetylcholinesterase activity and signal transduction molecule (cAMP) in tissues of Mytilus galloprovincialis (L.), in pollution monitoring. Mar. Environ. Res. 56 (2003) 443-470
6. Hamed S.S., Kauschke E., and Cooper E.L. Cytochemical properties of earthworm coelomocytes enriched by Percoll. In: Beschin A., Bilej M., and Cooper E.L. (Eds). A New Model for Analyzing Antimicrobial Peptides with Biomedical Applications (2002), IOS Press, Ohmsha 29-37
7. Koziol B., Markowicz M., Kruk J., and Plytycz B. Riboflavin as a source of autofluorescence in Eisenia fetida coelomocytes. Photochem. Photobiol. 82 (2006) 570-573
8. Kurek A., and Plytycz B. Annual changes in coelomocytes of four earthworm species. Pedobiologia 47 (2003) 689-701
9. Maboeta M.S., Reinecke S.A., and Reinecke A.J. Linking lysosomal biomarker and population responses in a field population of Aporrectodea caliginosa (Oligochaeta) exposed to the fungicide copper oxychloride. Ecotoxicol. Environ. Saf. 56 (2003) 411-418
10. Plytycz B., Homa J., Koziol B., Rozanowska M., and Morgan A.J. Riboflavin content in autofluorescent earthworm coelomocytes is species-specific. Folia Histochem. Cytobiol. 44 (2006) 65-71
11. Plytycz B., and Jozkowicz A. Differential effects of temperature on macrophages of ectothermic vertebrates. J. Leukoc. Biol. 56 (1994) 729-731
12. Plytycz B., Różanowska R., and Seljelid R. Quantification of neutral red pinocytosis by adherent cells: comparative studies. Folia Biol. (Kraków) 40 (1992) 3-9
13. Reinecke A.J., and Reinecke S.A. The influence of exposure history to lead on the lysosomal response in Eisenia fetida (Oligochaeta). Ecotoxicol. Environ. Saf. 55 (2003) 30-37
14. Roch P. Protein analysis of earthworm coelomic fluid: polymorphic system of the natural hemolysin of Eisenia fetida andrei. Dev. Comp. Immunol. 3 (1979) 599-608
15. Sipka S., Antal-Szalmás P., Szöllösi I., Csipö I., Lakos G., and Szegedi G. Ceramide stimulates the uptake of neutral red in human neutrophils, monocytes, and lymphocytes. Ann. Hematol. 79 (2000) 83-85
16. Svendsen C., Meharg A.A., Freestone P., and Weeks J.M. Use of an earthworm lysosomal biomarker for the ecological assessment of pollution from an industrial plastics fire. Appl. Soil Ecol. 3 (1996) 99-107
17. Svendsen C., Spurgeon D.J., Handkard P.K., and Weeks J.M. A review of lysosomal membrane stability measured by neutral red retention: is it a workable earthworm biomarker?. Ecotoxicol. Environ. Saf. 57 (2004) 20-29