Measurement of sarcoplasmic reticulum Ca2+ ATPase activity using high-performance liquid chromatography

Analytical Biochemistry

Volumn 372, Issue 2, 2008, Pages 135-139

  Williams, Jay H ^a   Vidt, Stacey E ^a   Rinehart, Janet ^a  

^a VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

Author keywords

ATPase; Calcium; Metabolism; SERCA; Skeletal muscle

Indexed keywords

CALCIUM; CHROMATOGRAPHIC ANALYSIS; ENZYMES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; MUSCLE;

ATP-ASE ACTIVITY; ATPASE; PERCHLORIC ACIDS; RELIABLE METHODS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC); SARCOPLASMIC RETICULUM; SERCA; SKELETAL MUSCLE;

CHROMIUM COMPOUNDS;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATASE (CALCIUM); ADENOSINE TRIPHOSPHATE; CALCIUM; CREATINE KINASE; CREATINE PHOSPHATE; PERCHLORIC ACID;

ANIMAL TISSUE; ARTICLE; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; RAT; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SARCOPLASMIC RETICULUM; SKELETAL MUSCLE;

EID: 36148989575     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.09.020     Document Type: Article

References (16)

1. 2+)-stimulated ATPase in human erythrocyte spectrin. Biochim. Biophys. Acta 401 (1975) 51-58
2. 2+ -ATPase of rat muscle. Biochem. Med. Metab. Biol. 46 (1991) 391-405
3. Carter S.G., and Karl D.W. Inorganic phosphate assay with malachite green: An improvement and evaluation. J. Biochem. Biophys. Methods 7 (1982) 7-13
4. Kodama T., Fukui K., and Kometani K. The initial phosphate burst in ATP hydrolysis by myosin and subfragment-1 as studied by a modified malachite green method for determination of inorganic phosphate. J. Biochem. (Tokyo) 99 (1986) 1465-1472
5. 2+ -ATPase. Eur. J. Biochem. 213 (1993) 973-980
6. 2+ uptake and regulate local ATP/ADP ratios. J. Biol. Chem. 265 (1990) 5258-5266
7. Han J.W., Thieleczek R., Varsanyi M., and Heilmeyer Jr. L.M. Compartmentalized ATP synthesis in skeletal muscle triads. Biochemistry 31 (1992) 377-384
8. Bernocchi P., Ceconi C., Cargnoni A., Pedersini P., Curello S., and Ferrari R. Extraction and assay of creatine phosphate, purine, and pyridine nucleotides in cardiac tissue by reversed-phase high-performance liquid chromatography. Anal. Biochem. 222 (1994) 374-379
9. 2+ uptake supported by endogenously synthesized ATP. Am. J. Physiol. Cell Physiol. 286 (2004) C97-C104
10. Williams J.H., Ward C.W., Spangenburg E.E., Nelson R., Stavrianeas S., and Klug G.A. Glucose-6-phosphate alters rat skeletal muscle contractile apparatus and sarcoplasmic reticulum function. Exp. Physiol. 83 (1998) 489-502
11. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
12. Tombes R.M., and Shapiro B.M. Metabolite channeling: A phosphorylcreatine shuttle to mediate high energy phosphate transport between sperm mitochondrion and tail. Cell 41 (1985) 325-334
13. Samizo K., Ishikawa R., Nakamura A., and Kohama K. A highly sensitive method for measurement of myosin ATPase activity by reversed-phase high-performance liquid chromatography. Anal. Biochem. 293 (2001) 212-215
14. Sudo J., Terui J., Iwase H., and Kakuno K. Assay of ATPase and Na,K-ATPase activity using high-performance liquid chromatographic determination of ADP derived from ATP. J. Chromatogr. B 744 (2000) 19-23
15. Ushimaru M., and Fukushima Y. Complete separation of adenine nucleotides for ATPase activity assay by ion-pair reversed-phase high-performance liquid chromatography. Anal. Biochem. 313 (2003) 173-175
16. 2+ pump function. Am. J. Physiol. 267 (1994) C357-C366