In vivo construction of recombinant molecules within the Caenorhabditis elegans germ line using short regions of terminal homology

Nucleic Acids Research

Volumn 35, Issue 19, 2007, Pages

  Kemp, Benedict J ^a   Hatzold, Julia ^a   Sternick, Laura A ^a   Cornman Homonoff, Joshua ^a   Whitaker, Jessica M ^a   Tieu, Pamela J ^a   Lambie, Eric J ^a  

^a Dartmouth College   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; OLIGONUCLEOTIDE; CIRCULAR DNA; GREEN FLUORESCENT PROTEIN; RECOMBINANT DNA;

ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; ENHANCER REGION; GENETIC ANALYSIS; GERM LINE; HOMOLOGOUS RECOMBINATION; IN VIVO STUDY; MOLECULAR INTERACTION; NONHUMAN; PHOSPHORYLATION; PRIORITY JOURNAL; TRANSGENICS; ANIMAL; CHEMISTRY; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETICS; GERM CELL; METHODOLOGY; POLYMERASE CHAIN REACTION; PROMOTER REGION; SEQUENCE HOMOLOGY;

CAENORHABDITIS ELEGANS;

ANIMALS; CAENORHABDITIS ELEGANS; DNA, CIRCULAR; DNA, RECOMBINANT; ENHANCER ELEMENTS (GENETICS); GENETIC ENGINEERING; GERM CELLS; GREEN FLUORESCENT PROTEINS; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RECOMBINATION, GENETIC; SEQUENCE HOMOLOGY, NUCLEIC ACID;

EID: 36148952552     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkm857     Document Type: Article

References (17)

1. Hua,S.B., Qiu,M., Chan,E., Zhu,L. and Luo,Y. (1997) Minimum length of sequence homology required for in vivo cloning by homologous recombination in yeast. Plasmid, 38, 91-96.
2. Raymond,C.K., Sims,E.H. and Olson,M.V. (2002)-Linker-mediated recombinational subcloning of large DNA fragments using yeast. Genome Res., 12, 190-197.
3. Copeland,N.G., Jenkins,N.A. and Court,D.L. (2001) Recombineering: A powerful new tool for mouse functional genomics. Nat. Rev. Genet., 2, 769-779.
4. Dolphin,C.T. and Hope,I.A. (2006) Caenorhabditis elegans reporter fusion genes generated by seamless modification of large genomic DNA clones. Nucleic Acids Res., 34, e72.
5. Mello,C.C., Kramer,J.M., Stinchcomb,D. and Ambros,V. (1991) Efficient gene transfer in C, elegans: Extrachromosomal maintenance and integration of transforming sequences. EMBO J., 10, 3959-3970.
6. Boulin,J., Etchberg,J.F. and Hobert,O. (2006) Reporter gene fusions. In Ambros,V. (ed.), WormBook. http://www.wormbook.org. doi/10.1895/ wormbook.1.106.1.
7. Brenner,S. (1974) The genetics of Caenorhabditis elegans. Genetics 77, 71-94.
8. Sun,A.Y. and Lambie,E.J. (1997) gon-2, a gene required for gonadogeriesis in Caenorhabditis elegans. Genetics, 147, 1077-1089.
9. Casadaban,M.J., Martinez-Arias,A., Shapira,S.K. and Chaou,J. (1993) β-Galactosidase gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol., 100, 293-299.
10. Fire,A., Harrison,S.W. and Dixon,D. (1990) A modular set of lacZ fusion vectors for studying gene expression in Caenorhabditis elegans. Gene 93, 189-198.
11. Stinchcomb,D.T., Shaw,J.E., Carr,S.H. and Hirsh,D. (1985) Extrachromosomal DNA transformation of Caenorhabditis elegans. Mol. Cell. Biol., 5, 3484-3496.
12. Hope,I.A. (1994) PES-1 is expressed during early embryogenesis in Caenorhabditis elegans and has homology to the fork head family of transcription factors. Development, 120, 505-514.
13. Mathies,L.D., Henderson,S.T. and Kimble,J. (2003) The C. elegans hand gene controls embryogenesis and early gonadogenesis. Development 130, 2881-2892.
14. Hobert,O. (2002) PCR fusion-based approach to create reporter gene constructs for expression analysis in transgenic C. elegans. Biotechniques, 32, 728-730.
15. Sassi,H.E., Renihan,S., Spence,A.M. and Cooperstock,R.L. (2005) Gene CATCHR - gene cloning and tagging for Caenorhabditis elegans using yeast homologous recombination: A novel approach for the analysis of gene expression. Nucleic Acids Res., 33, e163.
16. Hope,I.A., Stevens,J., Garner,A., Hayes,J., Cheo,D.L,, Brasch,M.A. and Vidal,M. (2004) Feasibility of genome-scale construction of promoter:rEporter gene fusions for expression in Caenorhabditis elegans using a multisite gateway recombination system. Genome Res., 14, 2070-2075.
17. Moore,D.S. and McCabe,G.P. (1998) Introduction to the Practice of Statistics. W.H. Freeman, New York, USA.