Evaluation of pan-fungal PCR assay and Aspergillus antigen detection in the diagnosis of invasive fungal infections in high risk paediatric cancer patients

Medical Mycology

Volumn 44, Issue 8, 2006, Pages 733-739

  El Mahallawy, Hadir A ^a   Shaker, Heba H ^a   Ali Helmy, Hala ^b   Mostafa, Tarek ^c   Abo Sedah, Abdel Razak ^c  

^a NATIONAL CANCER INSTITUTE   (Egypt)

^b CAIRO UNIVERSITY   (Egypt)

^c Assiut Branch   (Egypt)

Author keywords

Aspergillus antigen; Galactomannan; Haemato oncology patients; Invasive fungal infection; Polymerase chain reaction

Indexed keywords

AMPHOTERICIN B; ANTIBIOTIC AGENT; ANTIFUNGAL AGENT; ANTINEOPLASTIC AGENT; FUNGUS ANTIGEN; GALACTOMANNAN;

ADOLESCENT; ADULT; ANTIGEN DETECTION; ARTICLE; ASPERGILLUS; BACTERIUM CULTURE; BLOOD CULTURE; CANCER PATIENT; CHILD; CHILDHOOD CANCER; COMPUTER ASSISTED TOMOGRAPHY; CONTROLLED STUDY; DIAGNOSTIC ERROR; DIAGNOSTIC VALUE; EARLY DIAGNOSIS; FEBRILE NEUTROPENIA; FUNGUS CULTURE; HIGH RISK PATIENT; HUMAN; MAJOR CLINICAL STUDY; NONHUMAN; OPPORTUNISTIC INFECTION; POLYMERASE CHAIN REACTION; PREDICTION; SENSITIVITY AND SPECIFICITY; SYSTEMIC MYCOSIS;

ADOLESCENT; ANTIGENS, FUNGAL; ASPERGILLOSIS; ASPERGILLUS; BLOOD; CHILD; CHILD, PRESCHOOL; EGYPT; ELECTROPHORESIS, AGAR GEL; HUMANS; MANNANS; MYCOSES; NEOPLASMS; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; SENSITIVITY AND SPECIFICITY; STATISTICS;

ASPERGILLUS; ASPERGILLUS GALACTOMANNAN; BACTERIA (MICROORGANISMS);

EID: 36049001225     PISSN: 13693786     EISSN: 14602709     Source Type: Journal    
DOI: 10.1080/13693780600939955     Document Type: Article

References (19)

1. Williamson, EC and Oliver, DA and Johnson, EM and (2000) Aspergillus antigen testing in bone marrow transplant recipients J Clin Pathol, 53, pp. 362-366.
2. Ulusakarya, A and Chachaty, E and Vantelon, JM and (2000) Surveillance of Aspergillus galactomannan antigenemia for invasive aspergillosis by enzyme-linked immunosorbent assay in neutropenic patients treated for haematological malignancies Hematol J, 1, pp. 111-116.
3. Mclintock, LA and Jones, BL. (2004) Advances in the molecular and serological diagnosis of invasive fungal infection in haemato-oncology patients Br J Haematol, 126, pp. 289-297.
4. Yamakami, Y and Hashimoto, A and Yamagata, E and (1998) Evaluation of PCR for detection of DNA specific for Aspergillus species in sera of patients with various forms of pulmonary aspergillosis J Clin Microbiol, 36, pp. 3619-3623.
5. Jones, BL and McLintock, LA. (2003) Impact of diagnostic markers on early antifungal therapy Curr Opin Infect Dis, 16, pp. 521-526.
6. el-Mahallawy, HA and Attia, N and Ali el-Din, N and Salem, A and Abo el-Naga, S. (2002) A prospective study on fungal infection in children with cancer J Med Microbiol, 51, pp. 601-605.
7. Sulhian, A and Talouret, M and Riboud, P and Sarfati, J. (1996) Comparison of an enzyme immunoassay and latex agglutination test for detection of galacomannan in the diagnosis of invasive aspergillosis Eur J Clin Microbiol Infect Dis, 15, pp. 139-145.
8. Einsele, H and Hebart, H and Roller, G and (1997) Detection and identification of fungal pathogens in blood by molecular probes J Clin Microbiol, 35, pp. 1353-1360.
9. Ascioglu, S and Rex, JH and de Pauw, W and (2002) Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: An international consensus Clin Infect Dis, 34, pp. 7-14.
10. Maertens, J and Verhaegen, J and Lagrou, K and Van Eldere, J and Boogaerts, M. (2001) Screening for circulating galactomannan as a noninvasive diagnostic tool for invasive aspergillosis in prolonged neutropenic patients and stem cell transplantation recipients: A prospective validation Blood, 97, pp. 1604-1610.
11. Verweij, PE and Meis, JF. (2000) Microbiological diagnosis of invasive fungal infections in transplant recipients Transpl Infect Dis, 2, pp. 80-87.
12. Kawazu, M and Kanda, Y and Nannya, Y and (2004) Prospective comparison of the diagnostic potential of real-time PCR, double-sandwich enzyme-linked immunosorbent assay for galactomannan, and a (1->3)-beta-D-glucan test in weekly screening for invasive aspergillosis in patients with hematological disorders J Clin Microbiol, 42, pp. 2733-2741.
13. Jordanides, NE and Allan, EK and Mclintock, LA and (2005) A prospective study of real-time panfungal PCR for the early diagnosis of invasive fungal infection in haemato-oncology patients Bone Marrow Transplant, 35, pp. 389-395.
14. Buchheidt, D and Baust, C and Skladny, H and (2001) Detection of Aspergillus species in blood and bronchoalveolar lavage samples from immunocompromised patients by means of 2-step polymerase chain reaction: clinical results Clin Infect Dis, 33, pp. 428-435.
15. Hebart, H and Loffler, J and Reitze, H and (2000) Prospective screening by a panfungal polymerase chain reaction assay in patients at risk for fungal infections: Implications for the management of febrile neutropenia Br J Haematol, 111, pp. 635-640.
16. Lass-Florl, C and Aigner, J and Gunsilius, E and (2001) Screening for Aspergillus spp. using polymerase chain reaction of whole blood samples from patients with haematological malignancies Br J Haematol, 113, pp. 180-184.
17. Loeffler, J and Hebart, H and Brauchle, U and Schumacher, U and Einsele, H. (2000) Comparison between plasma and whole blood specimens for detection of Aspergillus DNA by PCR J Clin Microbiol, 38, pp. 3830-3833.
18. Gangneux, JP and Lavarde, D and Bretagne, S and Guiguen, C and Gandemer, V. (2002) Transient Aspergillus antigenaemia: Think of milk Lancet, 359, pp. 1251.
19. Pinel, C and Fricker-Hidalgo, H and Lebeau, B and (2003) Detection of circulating Aspergillus fumigatus galactomannan: Value and limits of the Platelia test for diagnosing invasive aspergillosis J Clin Microbiol, 41, pp. 2184-2186.