The development of an ultra performance liquid chromatography-coupled atmospheric pressure chemical ionization mass spectrometry assay for seven adrenal steroids

Analytical Biochemistry

Volumn 372, Issue 1, 2008, Pages 11-20

  Storbeck, Karl Heinz ^a   Kolar, Norbert W ^a   Stander, Marietjie ^a   Swart, Amanda C ^a   Prevoo, Désirée ^a   Swart, Pieter ^a  

^a STELLENBOSCH UNIVERSITY   (South Africa)

Author keywords

Atmospheric pressure chemical ionization (APCI); Cytochrome P450 17 hydroxylase 17,20 lyase (CYP17); Ultra performance liquid chromatography (UPLC)

Indexed keywords

ATMOSPHERIC CHEMISTRY; ATMOSPHERIC IONIZATION; ATMOSPHERIC PRESSURE; IONIZATION OF LIQUIDS; LIPIDS; LIQUIDS; MASS SPECTROMETRY; METABOLITES; YEAST;

ATMOSPHERIC PRESSURE CHEMICAL IONIZATION; ATMOSPHERIC PRESSURE CHEMICAL IONIZATION MASS SPECTROMETRY; CYTOCHROME P450; DETECTION AND QUANTIFICATIONS; HETEROLOGOUS EXPRESSION; LIQUID-LIQUID EXTRACTION; TRIPLE-QUADRUPOLE MASS SPECTROMETER; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

LIQUID CHROMATOGRAPHY;

CORTICOSTEROID; CYTOCHROME P450 17;

ANIMAL CELL; ARTICLE; ASSAY; ATMOSPHERIC PRESSURE; CELL STRAIN COS1; CONTROLLED STUDY; EXTRACTION; ION MONITORING; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; STEROIDOGENESIS;

PICHIA PASTORIS;

EID: 36049000268     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.09.036     Document Type: Article

References (28)

1. Payne A.H., and Hales D.B. Overview of steroidogenic enzymes in the pathway from cholesterol to active steroid hormones. Endocr. Rev. 25 (2004) 947-970
2. Swart P., Swart A.C., Waterman M.R., Estabrook R.W., and Mason J.I. Progesterone 16α-hydroxylase activity is catalyzed by human cytochrome P450 17α-hydroxylase. J. Clin. Endocrinol. Metab. 77 (1993) 98-102
3. Arlt W., Martens J.W.M., Song M., Wang J.T., Auchus R.J., and Miller W.L. Molecular evolution of adrenarche: Structural and functional analysis of P450c17 from four primate species. Endocrinology 143 (2002) 4665-4672
4. Geller D.H., Auchus R.J., and Miller W.L. P450c17 mutations R347H and R358Q selectively disrupt 17,20-lyase activity by disrupting interactions with P450 oxidoreductase and cytochrome b5. Mol. Endocrinol. 13 (1999) 167-175
5. Pandey A.V., and Miller W.L. Regulation of 17,20-lyase activity by cytochrome b5 and by serine phosphorylation of P450c17. J. Biol. Chem. 280 (2005) 13265-13271
6. Yang W., and Hammes S.R. Xenopus laevis CYP17 regulates androgen biosynthesis independent of the cofactor cytochrome b5. J. Biol. Chem. 280 (2005) 10196-10201
7. Soucy P., and Luu-The V. Conversion of pregnenolone to DHEA by human 17α-hydroxylase/17,20-lyase (P450c17): Evidence that DHEA is produced from the released intermediate 17α-hydroxypregnenolone. Eur. J. Biochem. 267 (2000) 3243-3247
8. Swart P., Lombard N., Swart A.C., van der Merwe T., Murry B.A., Nicol M., and Mason J.I. Ovine steroid 17α-hydroxylase cytochrome P450: Characteristics of the hydroxylase and lyase activities of the adrenal cortex enzyme. Arch. Biochem. Biophys. 409 (2003) 145-152
9. Swart A.C., Kolar N.W., Lombard N., Mason J.I., and Swart P. Baboon cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17) characterization of the adrenal microsomal and cloned enzymes. Eur. J. Biochem. 269 (2002) 5608-5616
10. Mikšík I., Mikulíková K., Pácha J., Kučka M., and Deyl Z. Application of liquid chromatography-electrospray ionization mass spectrometry for study of steroid-converting enzymes. J. Chromatogr. B 800 (2004) 145-153
11. Escobar-Morreale H.F., San Millan J.L., Smith R.R., Sancho J., and Witchel S.F. The presence of the 21-hydroxylase deficiency carrier status in hirsute women: Phenotype-genotype correlations. Fertil. Steril. 72 (1999) 629-638
12. Traggiai C., and Stanhope R. Delayed puberty. Best Pract. Res. Clin. Endocrinol. Metab. 16 (2002) 139-151
13. Migeon C.J., Berkovitz G.D., and Brown T.R. Sexual differentiation and ambiguity. In: Kappy M.S., Blizzard R.M., and Migeon C.J. (Eds). The Diagnosis and Treatment of Endocrine Disorders in Childhood and Adolescence (1994), Charles C. Thomas, Springfield, IL 573-715
14. Lee P.A. Laboratory monitoring of children with precocious puberty. Arch. Pediatr. Adolesc. Med. 148 (1994) 369-376
15. Kulin K.E., Finkelstein J.W., D́Arcangelo M.R., Susman E.J., Chinchilli V., Kunselman S., Schwab J., Demers L., and Lookingbill G. Diversity of pubertal testosterone changes in boys: Constitutional delay in growth and/or adolescence. J. Pediatr. Endocrinol. Metab. 10 (1997) 395-400
16. Summers R.H., Herold D.A., and Seely B.L. Hormonal and genetic analysis of a patient with congenital adrenal hyperplasia. Clin. Chem. 42 (1996) 1483-1487
17. 13C]testosterone as internal standard. J. Chromatogr. 339 (1985) 233-242
18. Wheeler M.J., D'Souza A., Matadeen J., and Croos P. Ciba Corning ACS:180 testosterone assay evaluated. Clin. Chem. 42 (1996) 1445-1449
19. Kushnir M.M., Rockwood A.L., Roberts W.L., Pattison E.G., Bunker A.M., Fitzgerald R.L., and Meikle A.W. Performance characteristics of a novel tandem mass spectrometry assay for serum testosterone. Clin. Chem. 52 (2006) 120-128
20. Taieb J., Mathian B., Millot F., Patricot M.C., Mathieu E., Queyre N., Lacroix I., Somma-Delpero C., and Boudou P. Testosterone measured by 10 immunoassays and by isotope dilution gas chromatography-mass spectrometry in sera from 116 men, women, and children. Clin. Chem. 49 (2003) 1381-1395
21. Wheeler M.J., and Lowry C. Warning on serum testosterone measurement. Lancet 2 (1987) 514-515
22. Dorgan J.F., Fears T.R., McMahon R.P., Aronson Friedman L., Patterson B.H., and Greenhut S.F. Measurement of steroid sex hormones in serum: A comparison of radioimmunoassay and mass spectrometry. Steroids 67 (2002) 151-158
23. Middle J. Standardization of steroid hormone assays. Ann. Clin. Biochem. 35 (1998) 354-363
24. Valdes Jr. R., and Jortani S. Unexpected suppression of immunoassay results by cross-reactivity: Now a demonstrated cause for concern. Clin. Chem. 48 (2002) 405-406
25. Luppa P., and Neumeier D. Effect of sex-hormone globulin on no-extraction immunoassays for testosterone. Clin. Chem. 36 (1990) 172-173
26. Nithipatikom K., Holmes B.B., Isbell M.A., Hanke C.J., Gomez-Sanchez C.E., and Campbell W.B. Measurement of steroid synthesis in zona glomerulosa cells by liquid chromatography-electrospray ionization-mass spectrometry: Inhibition by nitric oxide. Anal. Biochem. 15 (2005) 203-210
27. N. K. Kolar, A. C. Swart, J. I. Mason, P. Swart, Functional expression and characterisation of human cytochrome P45017α inPichia pastoris, J. Biotechnol. (2007) doi:10.1016/j.jbiotec.2007.02.003.
28. Mikšík I., Vylitová M., Pácha J., and Deyl Z. Separation and identification of corticosterone metabolites by liquid chromatography-electrospray ionization mass spectrometry. J. Chromatogr. B 726 (1999) 59-69