Generation of diacylglycerol during the acrosome reaction induced by Ca2+ and Ca2+ lonophore A23187 in frozen-thawed spermatozoa from fertile and subfertile japanese black bulls

Journal of Reproduction and Development

Volumn 47, Issue 5, 2001, Pages 311-316

  Murase, Tetsuma ^a   Mukohjima, Koji ^b   Sakaguchi, Shin Ichi ^b   Ohtani, Tsuyoshi ^a   Tsubota, Toshio ^a  

^a GIFU UNIVERSITY   (Japan)

^b Gifu Prefectural Livestock Research Institute   (Japan)

Author keywords

Bull, ca2+, spermatozoa, acrosome reaction, diacylglycerol, fertility

Indexed keywords

EID: 36048958066     PISSN: 09168818     EISSN: 13484400     Source Type: Journal    
DOI: 10.1262/jrd.47.311     Document Type: Article

References (26)

1. Yanagimachi R. Mammalian Fertilization. In: Knobil E, Neill JD (eds.), The Physiology of Reproduction, 2nd ed. New York: Raven Press; 1994:189-317.
2. Roldan ERS, Murase T, Shi Q-X. Exocytosis in spermatozoa in response to progesterone and zona pellucida. Science 1994; 266:1578-1581.
3. Shams-Borhan G, Harrison RAP. Production, characterization, and use of ionophore-induced, calcium-dependent acrosome reaction in ram spermatozoa. Gamete Res 1981; 4: 407-432.
4. 2+-regulating mechanisms that modulate bull sperm capacitation and acrosomal exocytosis as determined by chlortetracycline analysis. Mol Reprod Dev 1995; 40: 233-241.
5. 2+ influx. Biochem ] 1989; 264: 539-546.
6. Roldan ERS, Murase T. Polyphosphoinositidederived diacylglycerol stimulates the hydrolysis of phosphatidylcholine by phospholipase C during exocytosis of the ram sperm acrosome. Effect is not mediated by protein kinase C. J Biol Chem 1994; 269: 23583-23589.
7. 2+/ionophoreinduced acrosome reaction of ram spermatozoa. J Biol Chem 1993; 268:13962-13970.
8. Stewart DL, O'Hagen C, Glover FA. The prediction of fertility of bull semen from laboratory tests. In: Proceedings of the 7th International Congress on Animal Reproduction and Artificial Insemination, Volume 11; 1972; Munich, Germany: 1280-1283.
9. Elliott FI. Significance of semen quality. In: Salisbury GW, VanDemark NL, Lodge, JR (eds.), Physiology of Reproduction and Artificial Insemination of Cattle. San Francisco: W. H. Freeman and Company; 1978: 428-441.
10. Garner DL. Ancillary tests of bull semen quality. Vet Clin Nor Am Food Anim Prac 1997; 13: 313-330.
11. Cummins JM, Pember SM, Jequier AM, Yovich JL, Hartmann PE. A test of the human sperm acrosome reaction following ionophore challenge. Relationship to fertility and other seminal parameters. J Androl 1991; 12: 98-103.
12. Whitfield CH, Parkinson TJ. Assessment of the fertilizing potential of frozen bovine spermatozoa by in vitro induction of acrosome reactions with calcium ionophore (A23187). Theriogenology 1995; 44: 413-422.
13. Januskauskas A, Johannisson A, Soderquist L, Rodriguez-Martinez H. Assessment of sperm characteristics post-thaw and response to calcium ionophore in relation to fertility in Swedish dairy AI bulls. Theriogenology 2000; 53: 859-875.
14. Francavilla F, Valenti M, Romano R, Santucci R, Marrone V. Relationship between acrosome reactions and hamster egg penetration after ionophore challenge in absence of teratozoospermia. Fertil Steril 1995; 63:1301-1305.
15. Beorlegui N, Cetica P, Trinchero G, Cordoba M, Beconi M. Comparative study of functional and biochemical parameters in frozen bovine sperm. Andrologia 1997; 29: 37-42.
16. Szász F, Sirivaidyapong S, Cheng FP, Voorhout WF, Marks A, Colenbrander B, Solti L, Gadella BM. Detection of calcium ionophore induced membrane changes in dog sperm as a simple method to predict the cryopreservability of dog semen. Mol Reprod Dev 2000; 55: 289-298.
17. Murase T, Mukohjima K, Sakaguchi S, Ohtani T, Tsubota T, Kita I. Characterization of frozenthawed Japanese black bull spermatozoa by standard semen analysis, mucus penetration test and the ability to undergo the acrosome reaction in response to calcium and the calcium ionophore A23187. J Reprod Dev 2001; 47: 237-243.
18. Murase T, Mukohjima K, Sakaguchi S, Tsubota T, Kita I. A simplified technique to determine the ability of spermatozoa to penetrate the estrous cervical mucus in Japanese beef cattle. J Reprod Dev 1999; 45: 425-428.
19. Roldan ERS, Harrison RAP. Absence of active protein kinase C in ram spermatozoa. Biochem Biophys Res Commun 1988; 155: 901-906.
20. Bligh EG, Dyer WJ. A rapid method for total lipid extraction and purification. Can J Biochem Physiol 1959; 37: 911-917.
21. Bocckino SP, Blackmore PF, Wilson PB, Exton JH. Phosphatidate accumulation in hormone-treated hepatocytes via a phospholipase D mechanism. J Bid Chem 1987; 262:15309-15315.
22. Nakamura K, Handa S. Coomassie brilliant blue staining of lipids on thin-layer plates. Anal Biochem 1984; 142: 406-410.
23. Roldan ERS, Harrison RAP. Diacylglycerol in the exocytosis of the mammalian sperm acrosome. Biochem Soc Trans 1993; 21: 284-289.
24. 2+/ ionophore-induced acrosome reaction of mammalian spermatozoa. Biochem }1989; 259: 397-406.
25. Nishizuka Y. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature 1984; 308, 693-698.
26. Garde J, Roldan ERS. rab3-Peptide stimulates exocytosis of the ram sperm acrosome via interaction with cyclic AMP and phospholipase A2 metabolites. FEES Lett 1996; 391: 263-268.