Porous thermoresponsive-co-biodegradable hydrogels as tissue-engineering scaffolds for 3-dimensional in vitro culture of chondrocytes

Tissue Engineering

Volumn 13, Issue 11, 2007, Pages 2645-2652

  Huang, Xiao ^a,c   Zhang, Yue ^a,b   Donahue, Henry J ^a,b   Lowe, Tao L ^a,b,d  

^a PENN STATE COLLEGE OF MEDICINE   (United States)

^b PENNSYLVANIA STATE UNIVERSITY   (United States)

^c Zimmer Orthobiologics Inc   (United States)

^d PENN STATE MILTON S HERSHEY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARTILAGE; ENZYME ACTIVITY; HYDROGELS; RNA; SCAFFOLDS; TISSUE ENGINEERING;

CHONDROCYTES; HYDROGEL SCAFFOLDS; POLYMERASE CHAIN REACTION; SALT LEACHING TECHNIQUE; THERMORESPONSIVE PROPERTIES;

CELL CULTURE;

COLLAGEN TYPE 10; COLLAGEN TYPE 2; DEXTRAN; LACTIC ACID; MESSENGER RNA; PHOSPHATE BUFFERED SALINE; POLY(N ISOPROPYLACRYLAMIDE); SODIUM CHLORIDE;

ANIMAL CELL; ARTICLE; BIODEGRADABILITY; CARTILAGE CELL; CELL CULTURE; CELL SWELLING; CONTROLLED STUDY; HEDGEHOG; HYDROGEL; IN VITRO STUDY; IN VIVO STUDY; LEACHING; NONHUMAN; PHENOTYPE; POROSITY; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TEMPERATURE SENSITIVITY; THERMAL STIMULATION; TISSUE ENGINEERING;

ANIMALS; BIOCOMPATIBLE MATERIALS; BIOLOGICAL MARKERS; BUFFERS; CELLS, CULTURED; CHICK EMBRYO; CHONDROCYTES; COLLAGEN TYPE II; COLLAGEN TYPE X; CROSS-LINKING REAGENTS; HEDGEHOG PROTEINS; HYDROGELS; HYDROGEN-ION CONCENTRATION; MODELS, BIOLOGICAL; ORGAN CULTURE TECHNIQUES; PHOSPHATES; POROSITY; RNA, MESSENGER; SOLUTIONS; STERNUM; TEMPERATURE; TIME FACTORS; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

ERINACEIDAE; STERNA;

EID: 35948987537     PISSN: 10763279     EISSN: None     Source Type: Journal    
DOI: 10.1089/ten.2007.0084     Document Type: Article

References (46)

1. Langer, R. Biomaterials in drug delivery and tissue engineering: one laboratory's experience. Accounts Chem Res 33, 94, 2000.
2. Brodkin, K.R., Garcia, A.J., and Levenston, M.E. Chondrocyte phenotypes on different extracellular matrix monolayers. Biomaterials 25, 5929, 2004.
3. Cao, Y.L., Rodriguez, A., Vacanti, M., Ibarra, C., Arevalo, C., and Vacanti, C.A. Comparative study of the use of poly(glycolic acid), calcium alginate and pluronics in the engineering of autologous porcine cartilage. J Biomat Sci Polym E 9, 475, 1998.
4. Stevens, M.M., Qanadilo, H.F., Langer, R., and Shastri, V.P. A rapid-curing alginate gel system: utility in periosteum-derived cartilage tissue engineering. Biomaterials 25, 887, 2004.
5. Deng, Y., Zhao, K., Zhang, X.F., Hu, P., and Chen, G.Q. Study on the three-dimensional proliferation of rabbit articular cartilage-derived chondrocytes on polyhydroxyalkanoate scaffolds. Biomaterials 23, 4049, 2002.
6. Grad, S., Zhou, L., Gogolewski, S., and Alini, M. Chondrocytes seeded onto poly (L/DL-lactide) 80%/20% porous scaffolds: a biochemical evaluation. J Biomed Mater Res A 66A, 571, 2003.
7. Kisiday, J., Jin, M., Kurz, B., Hung, H., Semino, C., Zhang, S., and Grodzinsky, A.J. Self-assembling peptide hydrogel fosters chondrocyte extracellular matrix production and cell division: Implications for cartilage tissue repair. Proc Natl Acad Sci U S A 99, 9996, 2002.
8. Bryant, S.J., and Anseth, K.S. Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels. J Biomed Mater Res 59, 63, 2002.
9. Bryant, S.J., Durand, K.L., and Anseth, K.S. Manipulations in hydrogel chemistry control photoencapsulated chondrocyte behavior and their extracellular matrix production. J Biomed Mater Res A 67A, 1430, 2003.
10. Bryant, S.J., Anseth, K.S., Lee, D.A., and Bader, D.L. Crosslinking density influences the morphology of chondrocytes photoencapsulated in PEG hydrogels during the application of compressive strain. J Orthop Res 22, 1143, 2004.
11. Zehbe, R., Libera, J., Gross, U., and Schubert, H. Short-term human chondrocyte culturing on oriented collagen coated gelatine scaffolds for cartilage replacement. Bio Med Mater Eng 15, 445, 2005.
12. Sontjens, S.H.M., Nettles, D.L., Carnahan, M.A., Setton, L.A., and Grinstaff, M.W. Biodendrimer-based hydrogel scaffolds for cartilage tissue repair. Biomacromolecules 7, 310, 2006.
13. Bryant, S.J., Davis-Arehart, K.A., Luo, N., Shoemaker, R.K., Arthur, J.A., and Anseth, K.S. Synthesis and characterization of photopolymerized multifunctional hydrogels: water-soluble poly(vinyl alcohol) and chondroitin sulfate macromers for chondrocyte encapsulation. Macromolecules 37, 6726, 2004.
14. Bryant, S.J., Bender, R.J., Durand, K.L., and Anseth, K.S. Encapsulating chondrocytes in degrading PEG hydrogels with high modulus: engineering gel structural changes to facilitate cartilaginous tissue production. Biotechnol Bioeng 86, 747, 2004.
15. Martens, P.J., Bryant, S.J., and Anseth, K.S. Tailoring the degradation of hydrogels formed from multivinyl poly(ethylene glycol) and poly(vinyl alcohol) macromers for cartilage tissue engineering. Biomacromolecules 4, 283, 2003.
16. Rice, M.A., and Anseth, K.S. Encapsulating chondrocytes in copolymer gels: Bimodal degradation kinetics influence cell phenotype and extracellular matrix development. J Biomed Mater Res A 70A, 560, 2004.
17. Collett, J., Crawford, A., Hatton, P.V., Geoghegan, M., and Rimmer, S. Thermally responsive polymeric hydrogel brushes: synthesis, physical properties and use for the culture of chondrocytes. J R Soc Interface 4, 117, 2007.
18. Cho, J.H., Kim, S.H., Park, K.D., Jung, M.C., Yang, W.I., Han, S.W., Noh, J.Y., and Lee, J.W. Chondrogenic differentiation of human mesenchymal stem cells using a thermosensitive poly(N-isopropylacrylamide) and water-soluble chitosan copolymer. Biomaterials 25, 5743, 2004.
19. An, Y.H., Webb, D., Gutowska, A., Mironov, V.A., and Friedman, R.J. Regaining chondrocyte phenotype in thermosensitive gel culture. Anat Rec 263, 336, 2001.
20. Au, A., Polotsky, A., Krzyminski, K., Gutowska, A., Hungerford, D.S., and Frondoza, C.G. Evaluation of thermoreversible polymers containing fibroblast growth factor 9 (FGF-9) for chondrocyte culture. J Biomed Mater Res A 69A, 367, 2004.
21. Chen, J.P., and Cheng, T.H. Thermo-responsive chitosangraft-poly(N- isopropylacrylamide) injectable hydrogel for cultivation of chondrocytes and meniscus cells. Macromol Biosci 6, 1026, 2006.
22. Na, K., Park, J.H., Kim, S.W., Sun, B.K., Woo, D.G., Chung, H.M., and Park, K.H. Delivery of dexamethasone, ascorbate, and growth factor (TGF beta-3) in thermo-reversible hydrogel constructs embedded with rabbit chondrocytes. Biomaterials 27, 5951, 2006.
23. Stile, R.A., Burghardt, W.R., and Healy, K.E. Synthesis and characterization of injectable poly(N-isopropylacrylamide)-based hydrogels that support tissue formation in vitro. Macromolecules 32, 7370, 1999.
24. Lee, B.H., West, B., McLemore, R., Pauken, C., and Vernon, B.L. In-situ injectable physically and chemically gelling NIPAAm-based copolymer system for embolization. Biomacromolecules 7, 2059, 2006.
25. Cellesi, F., Tirelli, N., and Hubbell, J.A. Materials for cell encapsulation via a new tandem approach combining reverse thermal gelation and covalent crosslinking. Macromol Chem Phys 203, 1466, 2002.
26. Vernon, B., and Martinez, A. Gel strength and solution viscosity of temperature-sensitive, in-situ-gelling polymers for endovascular embolization. J Biomat Sci Polym E 16, 1153, 2005.
27. Cellesi, F., and Tirelli, N. A new process for cell microencapsulation and other biomaterial applications: thermal gelation and chemical cross-linking in 'tandem'. J Mater Sci Mater Med 16, 559, 2005.
28. Karageorgiou, V., and Kaplan, D. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 26, 5474, 2005.
29. Spiteri, C.G., Pilliar, R.M., and Kandel, R.A. Substrate porosity enhances chondrocyte attachment, spreading, and cartilage tissue formation in vitro. J Biomed Mater Res A 78A, 676, 2006.
30. Huang, X., Nayak, B.R., and Lowe, T.L. Synthesis and characterization of novel thermoresponsive-co-biodegradable hydrogels composed of N-isopropylacrylamide, poly(L-lactic acid), and dextran. J Polym Sci Pol Chem 42, 5054, 2004.
31. Wu, Q.Q., Zhang, Y., and Chen, Q. Indian hedgehog is an essential component of mechanotransduction complex to stimulate chondrocyte proliferation. J Biol Chem 276, 35290, 2001.
32. Zhang, Y., and Chen, Q. Changes of matrilin forms during endochondral ossification - molecular basis of oligomeric assembly. J Biol Chem 275, 32628, 2000.
33. Asoh, T., Kaneko, T., Matsusaki, M., and Akashi, M. Rapid deswelling of semi-IPNs with nanosized tracts in response to pH and temperature. J Control Release 110, 387, 2006.
34. Zhang, X.Z., and Chu, C.C. Fabrication and characterization of microgel-impregnated, thermosensitive PNIPAAm hydrogels. Polymer 46, 9664, 2005.
35. Lee, W.F., and Yeh, Y.C. Effect of porosigen and hydrophobic monomer on the fast swelling-deswelling behaviors for the porous thermoreversible copolymeric hydrogels. J Appl Polym Sci 100, 3152, 2006.
36. Zhang, X.Z., Wang, F.J., and Chu, C.C. Thermoresponsive hydrogel with rapid response dynamics. J Mater Sci Mater Med 14, 451, 2003.
37. Zhang, X.Z., Wu, D.Q., and Chu, C.C. Synthesis and characterization of partially biodegradable, temperature and pH sensitive Dex-MA/PNIPAAm hydrogels. Biomaterials 25, 4719, 2004.
38. Serizawa, T., Wakita, K., and Akashi, M. Rapid deswelling of porous poly(N-isopropylacrylamide) hydrogels prepared by incorporation of silica particles. Macromolecules 35, 10, 2002.
39. Zhang, X.Z., Wu, D.Q., and Chu, C.C. Effect of the cross-linking level on the properties of temperature-sensitive poly(N-isopropylacrylamide) hydrogels. J Polym Sci [B] 41, 582, 2003.
40. Zhang, X.Z., and Chu, C.C. Dynamic studies on thermoresponsive poly(N-isopropylacrylamide) hydrogel in tetrahydrofuran/water mixtures. Colloid Polym Sci 282, 589, 2004.
41. Huang, X., and Lowe, T.L. Biodegradable thermoresponsive hydrogels for aqueous encapsulation and controlled release of hydrophilic model drugs. Biomacromolecules 6, 2131, 2005.
42. Yoon, J.J., and Park, T.G. Degradation behaviors of biodegradable macroporous scaffolds prepared by gas foaming of effervescent salts. J Biomed Mater Res 55, 401, 2001.
43. Makino, K., Hiyoshi, J., and Ohshima, H. Kinetics of swelling and shrinking of poly (N-isopropylacrylamide) hydrogels at different temperatures. Colloid Surf B Biointerfaces 19, 197, 2000.
44. Okay, O. Macroporous copolymer networks. Prog Polym Sci 25, 711, 2000.
45. Sayil, C., and Okay, O. Macroporous poly(N-isopropylacrylamide) networks. Polym Bull 48, 499, 2002.
46. Ng, L.J., Wheatley, S., Muscat, G.E.O., Conway-Campbell, J., Bowles, J., Wright, E., Bell, D.M., Tam, P.P.L., Cheah, K.S.E., and Koopman, P. SOX9 binds DNA, activates transcription, and coexpresses with type II collagen during chondrogenesis in the mouse. Dev Biol 183, 108, 1997.