Low-load compression testing: A novel way of measuring biofilm thickness

Applied and Environmental Microbiology

Volumn 73, Issue 21, 2007, Pages 7023-7028

  Paramonova, Ekaterina ^a   De Jong, Ed D ^a   Krom, Bastiaan P ^a   Van Der Mei, Henny C ^a   Busscher, Henk J ^a   Sharma, Prashant K ^a  

^a UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

COMPRESSION TESTING; CONFOCAL MICROSCOPY; DYES; MICROORGANISMS; THICKNESS MEASUREMENT;

CONFOCAL LASER SCANNING MICROSCOPY; FLUORESCENT DYES; LOW-LOAD COMPRESSION TESTING;

BIOFILMS;

FLUORESCENT DYE;

ABUNDANCE; BIOFILM; COMMUNITY DYNAMICS; COMPRESSION; MEASUREMENT METHOD; MICROBIAL COMMUNITY; MICROORGANISM; YEAST;

ANALYTIC METHOD; ARTICLE; BIOFILM; CANDIDA ALBICANS; COMPARATIVE STUDY; CONFOCAL LASER MICROSCOPY; ENTEROCOCCUS FAECALIS; LOW LOAD COMPRESSION TESTING; MEASUREMENT; MICROFLORA; NONHUMAN; PERMEABILITY; PSEUDOMONAS AERUGINOSA; STREPTOCOCCUS ORALIS; THICKNESS; YEAST;

BACTERIA; BIOFILMS; CELL CULTURE TECHNIQUES; FLUORESCENT DYES; MICROBIOLOGICAL TECHNIQUES; MICROSCOPY, CONFOCAL; YEASTS;

BACTERIA (MICROORGANISMS);

EID: 35948933488     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00935-07     Document Type: Article

References (18)

1. Bakke, R., R. Kommedal, and S. Kalvenes, 2001. Quantification of biofilm accumulation by optical approach. J. Microbiol. Methods 44:13-26.
2. Battin, T. J., L. A. Kaplan, J. D. Newbold, X. Cheng, and C. Hansen, 2003. Effects of current velocity on the nascent architecture of stream microbial biofilms. Appl. Environ. Microbiol. 69:5443-5452.
3. Busscher, H. J., and H. C. van der Mei. 2006. Microbial adhesion in flow displacement systems. Clin. Microbiol. Rev. 19:127-141.
4. Costerton, J. W., P. S. Stewart, and E. P. Greenberg. 1999. Bacterial biofilms: a common cause of persistent infections. Science 284:1318-1322.
5. Donlan, R. M., and J. W. Costerton. 2002. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin. Microbiol. Rev. 15:167-193.
6. Henry-Stanley, M. J., R. M. Garni, and C. L. Wells. 2004. Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast cells to adhere to and to be internalized by cultured mammalian cells. J. Microbiol. Methods 59:289-292.
7. Heydorn, A., A. T. Nielsen, M. Hentzer, C. Sternberg, M. Giskov, B. K. Ersboll, and S. Molin. 2000. Quantification of biofilm structures by the novel computer program COMSTAT. Microbiology 146:2395-2407.
8. Hu, Z., G. Hidalgo, P. L. Houston, A. G. Hay, M. L. Shuler, H. D. Abruna, W. C. Ghiorse, and L. W. Lion. 2005. Determination of spatial distributions of zinc and active biomass in microbial biofilms by two-photon laser scanning microscopy. Appl. Environ. Microbiol. 71:4014-4021.
9. Korstgens, V., H. C. Flemming, J. Wingender, and W. Borchard. 2001. Influence of calcium ions on the mechanical properties of a model biofilm of mucoid Pseudomonas aeruginosa. Water Sci. Technol. 13:49-57.
10. Korstgens, V., H. C. Flemming, J. Wingender, and W. Borchard. 2001. Uniaxial compression measurement device for investigation of the mechanical stability of biofilms. J. Microbiol. Methods 46:9-17.
11. Krom, B. P., J. B. Cohen, G. E. McEIhaney Feser, and R. L. Cihlar. 2007. Optimized candidal biofilm microtiter assay. J. Microbiol. Methods 68:421-423.
12. Manz, B., F. Volke, D. Goll, and H. Horn. 2003. Measuring local flow velocities and biofilm structure in biofilm systems with magnetic resonance imaging (MRI). Biotechnol. Bioeng. 84:424-432.
13. Milferstedt, K., M. N. Pons, and E. Morgenroth. 2006. Optical method for long-term and large-scale monitoring of spatial biofilm development. Biotechnol. Bioeng. 94:773-782.
14. Okkerse, W. J., S. P. Ottengraf, and B. Osinga-Kuipers. 2000. Biofilm thickness variability investigated with a laser triangulation sensor. Biotechnol. Bioeng. 70:619-629.
15. Stewart, P. S. 2003. Diffusion in biofilms. J. Bacteriol. 185:1485-1491.
16. Stoodley, P., J. D. Boyle, D. DeBeer, and H. M. Lappin-Scott. 1999. Evolving perspectives of biofilm structure. Biofouling 14:75-90.
17. Vroom, J. M., K. J. De Grauw, H. C. Gerritsen, D. J. Bradshaw, P. D. Marsh, G. K. Watson, J. J. Birmingham, and C. Allison. 1999. Depth penetration and detection of pH gradients in biofilms by two-photon laser scanning microscopy. Appl. Environ. Microbiol. 65:3502-3511.
18. Waar, K., A. B. Muscholl-Siberhorn, R. J. L. Willems, M. J. H. Slooff, H. J. M. Harmsen, and J. E. Degener. 2002. Genogrouping and incidence of virulence factors of Enterococcus faecalis in liver transplant patients differ from blood culture and fecal isolates. J. Infect. Dis. 185:1121-1127.