In vitro approaches for the study of molecular motors in aster formation

Methods in Cell Biology

Volumn 67, Issue 67, 2001, Pages 225-239

  Compton, Duane A ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DYNEIN ADENOSINE TRIPHOSPHATASE;

ANIMAL; ARTICLE; CELL CULTURE; CELL FREE SYSTEM; CELL LINE; CHEMISTRY; CYTOLOGY; CYTOPLASM; HELA CELL; HUMAN; METABOLISM; MICROTUBULE; PHYSIOLOGY; ULTRASTRUCTURE;

ANIMALS; CELL LINE; CELL-FREE SYSTEM; CELLS, CULTURED; CYTOLOGICAL TECHNIQUES; CYTOPLASM; DYNEIN ATPASE; HELA CELLS; HUMANS; MICROTUBULES;

EID: 35848967399     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s0091-679x(01)67016-x     Document Type: Article

References (64)

1. Bastmeyer, M., Steffen, W., and Fuge, H. (1986). Immunostaining of spindle components in tipulid spematocytes using a serum against pericentriolar material. Eur. J. Cell Biol. 42, 305-310.
2. Blangy, A., Lane, H. A., d'Herin, P., Harper, M., Kress, M., and Nigg, E. A. (1995). Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell 83, 1159-1169.
3. Brenner, S., Branch, A., Meredith, S., and Berns, M. W. (1977). The absence of centrioles from spindle poles of rat kangaroo (PtK2) cells undergoing meiotic-like reduction division in vitro. J. Cell Biol. 72, 368-379.
4. Compton, D. A. (1998). Focusing on spindle poles. J. Cell Sci. 111, 1477-1481.
5. Compton, D. A. (2000). Spindle assembly in animal cells. Anna. Rev. Biochem. 69, 95-114.
6. Compton, D. A., and Cleveland, D. W. (1994). NuMA, a nuclear protein involved in mitosis and nuclear reformation. Curr. Opin. Cell Biol. 6, 343-346.
7. Debec, A., Détraves, C., Montmory, C., Géraud, G., and Wright, M. (1995). Polar organization of gamma-tubulin in acentriolar mitotic spindles of Drosophila melanogaster cells. J. Cell Sci. 108, 2645-2653.
8. de Saint Phalle, B., and Sullivan, W. (1998). Spindle assembly and mitosis without centrosomes in partheno-genetic Scaria embryos. J. Cell Biol. 141, 1383-1391.
9. Dionne, M. A., Howard, L., and Compton, D. A. (1999). NuMA is a component of an insoluble matrix at mitotic spindle poles. Cell Motil. Cytoskel. 42, 189-203.
10. Echeverri, C. J., Paschal, B. M., Vaughan, K. T., and Vallee, R. B. (1996). Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis. J. Cell Biol. 132, 617-633.
11. Endow, S. A, Chandra, R., Komma, D. J., Yamamoto, A. H., and Salmon, E. D. (1994). Mutants of the Drosophila ncd microtubule motor protein cause centrosomal and spindle defects in mitosis. J. Cell Sci. 107, 859-867.
12. Endow, S. A., and Komma, D. J. (1996). Centrosome and spindle function of the Drosophila Ncd microtubule motor visualized in live embryos using Ncd-GFP fusion proteins. J. Cell Sci. 109, 2429-2442.
13. Gaglio, T., Dionne, M. A., and Compton, D. A. (1997). Mitotic spindle poles are organized by structural and motor proteins in addition to centrosomes. J. Cell Biol. 138, 1055-1066.
14. Gaglio, T., Saredi, A., Bingham, J. B., Hasbani, M. J., Gill, S. R., Schroer, T. A., and Compton, D. A. (1996). Opposing motor activities are required for the organization of the mammalian mitotic spindle pole. J. Cell Biol. 135, 399-414.
15. Gaglio, T., Saredi, A., and Compton, D. A. (1995). NuMA is required for the organization of microtubules into aster-like mitotic arrays. J. Cell Biol. 131, 693-708.
16. Heald, R., Tournebize, R., Blank, T., Sandaltzopoulos, R., Beker, P., Hyman, A., and Karsenti, E. (1996). Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382, 420-125.
17. Heald, R., Tournebize, R., Habermann, A., Karsenti, E., and Hyman, A. (1997). Spindle assembly in Xenopus egg extracts: Respective roles of centrosomes and microtubule self-organization. J. Cell Biol. 138, 615-628.
18. Heck, M. M. S., Pereira, A., Pesavento, P., Yannoni, Y., Spradling, A. C., and Goldstein, L. S. B. (1993). The kinesin-like protein KLP61F is essential for mitosis in Drosophila. J. Cell Biol. 123, 665-679.
19. Keyer, G., Ris, H., and Borisy, G. G. (1984). Centriole distribution during tripolar mitosis in Chinese hamster ovary cells. J. Cell Biol. 98, 2222-2229.
20. Khodjakev, A., Cole, R. W., Oakley, B. R., and Rieder, C. L. (2000). Centrosome-independent mitotic spindle formation in vertebrates. Curr. Biol. 10, 59-67.
21. Kimble, M., and Church, K. (1983). Meiosis and early cleavage in drosophila melanogaster eggs: Effects of the claret-non-disjunctional mutation. J. Cell Sci. 62, 301-318.
22. Lohka, M. J., and Maller, J. L. (1985). Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts. J. Cell Biol. 101, 518-523.
23. Maniotis, A., and Schliwa, M. (1991). Microsurgical removal of centrosomes blocks cell reproduction and centriole generation in BSC-1 cells. Cell 67, 495-504.
24. Mastronarde, D. N., McDonald, K. L., Ding, R., and McIntosh, J. R. (1993), Interpolar spindle microtubules in PTK cells. J. Cell Biol. 123, 1475-1489.
25. Matthies, H. J. G., McDonald, H. B., Goldstein, L. S. B., and Theurkauf, W. E. (1996). Anastral meiotic spindle morphogenesis: Role of the non-claret disjunctional kinesin-like protein. J. Cell Biol. 134, 455-464.
26. Mazia, D. (1984). Centrosomes and mitotic poles. Exp. Cell Res. 153, 1-15.
27. McDonald, K. L., O'Toole, E. T., Mastronarde, D. N., and McIntosh, J. R. (1992). Kinetochore microtubules in PTK cells. J. Cell Biol 118, 369-383.
28. McIntosh, J. R. (1983). The centrosome as organizer of the cytoskeleton. Modern Cell Biol. 2, 115-142.
29. McIntosh, J. R., and Koonce, M. P. (1989). The mitotic spindle. Science 246, 622-628.
30. McKim, K. S., and Hawley, R. S. (1995). Chromosomal control of meiotic cell division. Science 270, 1595-1601.
31. Meluh, P. B., and Rose, M. D. (1990). KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell 60, 1023-1041.
32. Merdes, A., and Cleveland, D. W. (1997). Pathways of spindle pole formation: Different mechanisms; conserved components. J. Cell Biol. 138, 953-956.
33. Merdes, A., Ramyar, K., Vechio, J. D., and Cleveland, D. W. (1996). A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly. Cell 87, 447-458.
34. Mitchison, T. J. (1989). Mitosis: Basic concepts. Curr. Opin. Cell Biol. 1, 67-74.
35. Mitchison, T. J., and Salmon, E. D. (1992). Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis. J. Cell Biol. 119, 569-582.
36. Mountain, V., Simerly, C., Howard, L., Ando, A., Schatten, G., and Compton, D. A. (1999). The kinesin-related protein, HSET, opposes the activity of Eg5 and cross-links microtubules in the mammalian mitotic spindle. J. Cell Biol. 147, 351-365.
37. Murray, A. W. (1991). Cell cycle extracts. Methods Cell Biol. 36, 581-605.
38. Murray, A. W., Desai, A. B., and Salmon, E. D. (1996). Real time observation of anaphase in vitro. Proc. Natl. Acad. Sci. USA 93, 12327-12332.
39. Nedelec, F. J., Surrey, T., Maggs, A. C., and Leibler, S. (1997). Self organization of microtubules and motors. Nature 389, 305-308.
40. Nicklas, R. B. (1989). The motor for poleward chromosome movement in anaphase is in or near the kinetochore. J. Cell Biol. 109, 2245-2255.
41. Nicklas, R. B., Kubai, D. F., and Hays, T. S. (1982). Spindle microtubules and their mechanical associations after micromanipulation in anaphase. J. Cell Biol. 95, 91-104.
42. Nicklas, R. B., Lee, G. M., Rieder, C. L., and Rupp, G. (1989). Mechanically cut mitotic spindles: Clean cuts and stable microtubules. J. Cell Sci. 94, 415-423.
43. Palazzo, R. E., Vaisberg, E. A., Weiss, D. G., Kuznetsov, S. A., and Steffen, W. (1999). Dynein is required for spindle assembly in cytoplasmic exracts of Spisula solidissima oocytes. J. Cell Sci. 112, 1291-1302.
44. Rieder, C. L. (1981). The structure of the cold-stable kinetochore fiber in metaphase PtK1 cells. Chromosoma (Berlin) 84, 145-158.
45. Rieder, C. L. (1991). Mitosis: Towards a molecular understanding of chromosome behavior. Curr. Opin. Cell Biol. 3, 59-66.
46. Rieder, C. L., Ault, J. G., Eicenlaub-Ritter, U., and Sluder, G. (1993). Morphogenesis of the mitotic and meiotic spindle: Conclusions obtained from one system are not necessarily applicable to the other. In "Chromosome Segregation and Aneuploidy" (B. K. Vig and A. Kappas, eds.), pp. 183-197. Springer-Verlag, New York.
47. Rieder, C. L., and Hard, R. (1990). Newt lung epithelial cells: Cultivation, use, and advantages for biomedical research. Int. Rev. Cell Biol. 122, 153-220.
48. Sawin, K. E., LeFuellec, K., Philippe, M., and Mitchison, T. J. (1992). Mitotic spindle organization by a plus-end-directed microlubule motor. Nature (Lond.) 359, 540-543.
49. Schatten, G. (1994). The centrosome and its mode of inheritance: The reduction of the centrosome during gametogenesis and ils restoration during fertilization. Dev. Biol. 165, 299-335.
50. Sharp, D. J., McDondald, K. L., Brown, H. M., Matthies, H. J., Walczak, C., Vale, R. D., Mitchison, T. J., and Scholey, J. M. (1999a). The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles. J. Cell Biol. 144, 125-138.
51. Sharp, D. J., Yu, K. R., Sisson, J. C., Sullivan, W., and Scholey, J. M. (1999b). Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosophila early embryos. Nature Cell Biol. 1, 51-54.
52. Sluder, G., and Rieder, C. L. (1985). Experimental separation of pronuclei in fertilized sea urchin eggs: Chromosomes do not organize a spindle in the absence of centrosomes. J. Cell Biol. 100, 897-903.
53. Smirnova, E. A., and Beyer, A. S. (1992). Spindle poles in higher plant mitosis. Cell Motil. Cytoskel 23, 1-7.
54. Steffen, W., Fuge, H., Dietz, R., Bastmeyer, M., and Müller, G (1986). Aster-free spindle poles in insect spermatocytes: Evidence for chromosome-induced spindle formation. J. Cell Biol. 102, 1679-1687.
55. Szollosi, D., Calarco, P., and Donahue, R. P. (1972). Absence of centrioles in the first and second meiotic spindles of mouse oocytes. J. Cell Sci. 11, 521-541.
56. Theurkauf, W. E., and Hawley, R. S. (1992). Meiotic spindle assembly in Drosophila females: Behavior of nonexchange chromosomes and the effects of mutations in the nod kinesin-related protein. J. Cell Biol. 116, 1167-1180.
57. Vaisberg, E. A., Koonce, M. P., and McIntosh, J. R. (1993). Cytoplasmic dynein plays a role in mammalian mitotic spindle formation. J. Cell Biol. 123, 849-858.
58. Verde, F., Berrez, J.-M., Antony, C., and Karsenti, E. (1991). Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: Requirement for phosphorylated factors and cytoplasmic dynein. J. Cell Biol. 112, 1177-1187.
59. Vernos, I., and Karsenti, E. (1995). Chromosomes take the lead in spindle assembly. Trends Cell Biol. 5, 297-301.
60. Walczak, C. E., Verma, S., and Mitchison, T. J. (1997). XCTK2: A kinesin-related protein that promotes mitotic spindle assembly in Xenopus laevis egg extracts. J. Cell Biol. 136, 859-870.
61. Walczak, C. E., Vernos, I., Mitchison, T. J., Karsenti, E., and Heald, R. (1998). A model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity. Curr. Biol. 8, 903-913.
62. Wilson, P. G., Fuller, M. T., and Borisy, G. G. (1997). Monastral bipolar spindles: Implications for dynamic centrosome organization. J. Cell Sci. 110, 451-464.
63. Wolf, K. W., and Bastmeyer, M. (1991). Cytology of Lepidoptera. V. The microtubule cytoskeleton in eupyrene spermatocytes of Ephistia kuehniella (pyralidae), Inachis io (Nymphlidae) and Orgyia antiqua (Lymantriidae). Eur. J. Cell Biol. 55, 225-237.
64. Zhang, D., and Nicklas, R. B. (1995). The impact of chromosomes and centrosomes on spindle assembly as observed in living cells. J. Cell Biol. 129, 1287-1300.