Ionic currents and ion fluxes in Neurospora crassa hyphae

Journal of Experimental Botany

Volumn 58, Issue 12, 2007, Pages 3475-3481

  Lew, Roger R ^a  

^a YORK UNIVERSITY   (Canada)

Author keywords

Current clamp; Filamentous fungi; Ion selective microelectrodes; K+, Cl , H +, and Ca2+ fluxes; Voltage clamp; Walled turgid cells

Indexed keywords

CURRENT DENSITY; FUNGI; MICROELECTRODES; MORPHOLOGY; VOLTAGE CONTROL;

CURRENT CLAMP; FILAMENTOUS FUNGI; ION-SELECTIVE MICROELECTRODES; VOLTAGE CLAMP; WALLED TURGID CELLS;

CYTOLOGY;

ARTICLE; ION SELECTIVE ELECTRODE; ION TRANSPORT; METABOLISM; MICROELECTRODE; NEUROSPORA CRASSA;

ION TRANSPORT; ION-SELECTIVE ELECTRODES; MICROELECTRODES; NEUROSPORA CRASSA;

FUNGI; NEUROSPORA; NEUROSPORA CRASSA;

EID: 35848940506     PISSN: 00220957     EISSN: 14602431     Source Type: Journal    
DOI: 10.1093/jxb/erm204     Document Type: Article

References (25)

1. Arif I, Newman IA, Keenlyside N. 1995. Proton flux measurements from tissues in buffered solutions. Plant, Cell and Environment 18, 1319-1324.
2. + transport by Arabidopsis root hairs at low pH. Australian Journal of Plant Physiology 28, 635-641.
3. Fuster D, Moe OW, Hilgemann DW. 2004. Lipid- and mechano-sensitivities of sodium/hydrogen exchangers analyzed by electrical methods. Proceedings of the National Academy of Sciences, USA 101, 10482-10487.
4. 2+ fluxes and current. The Plant Journal 46, 134-144.
5. Gradmann D, Hansen U-P, Long WS, Slayman CL, Warncke J. 1978. Current-voltage relationships for the plasma membrane and its principal electrogenic pump in Neurospora crassa: I. Steady-state conditions. Journal of Membrane Biology 39, 333-367.
6. Henriksen GH, Raj Raman D, Walker LP, Spanswick RM. 1992. Measurement of net fluxes of ammonium and nitrate at the surface of barley roots using ion-selective microelectrodes. II. Patterns of uptake along the root axis and evaluation of the microelectrode flux estimation technique. Plant Physiology 99, 734-747.
7. Kang TM, Markin VS, Hilgemann DW. 2003. Ion fluxes in giant excised cardiac membrane patches detected and quantified with ion-selective microelectrodes. Journal of General Physiology 121, 325-347.
8. 2+ exchanger. Nature 427, 544-548.
9. Lew RR. 1991. Electrogenic transport properties of growing Arabidopsis root hairs: the plasma membrane proton pump and potassium channels. Plant Physiology 97, 1527-1534.
10. Lew RR. 1994. Regulation of electrical coupling between Arabidopsis root hairs. Planta 193, 67-73.
11. Lew RR. 1996. Pressure regulation of the electrical properties of growing Arabidopsis thaliana L. root hairs. Plant Physiology 112, 1089-1100.
12. + flux magnitude and location along growing hyphae of Saprolegnia ferax and Neurospora crassa. European Journal of Cell Biology 78, 892-902.
13. Lew RR. 2005. Mass flow and pressure-driven hyphal extension in Neurospora crassa. Microbiology 151, 2685-2692.
14. Lew RR. 2006. Use of double barrel micropipettes to voltage-clamp plant and fungal cells. In: Volkov AG, ed. Plant electrophysiology - theory and methods. Heidelberg: Springer, 139-154.
15. Lew RR, Levina NN, Shabala L, Anderca MI, Shabala SN. 2006. Role of a mitogen-activated protein kinase cascade in ion flux-mediated turgor regulation in fungi. Eukaryotic Cell 5, 480-487.
16. Lew RR, Levina NN, Walker SK, Garrill A. 2004. Turgor regulation of hyphal organisms. Fungal Genetics and Biology 41, 1007-1015.
17. McCluskey K. 2003. The Fungal Genetics Stock Center: from molds to molecules. Advances in Applied Microbiology 52, 245-262.
18. Messerli MA, Robinson KR, Smith PJS. 2006. Electrochemical sensor applications to the study of molecular physiology and analyte flux in plants. In: Volkov AG, ed. Plant electrophysiology - theory and methods. Heidelberg: Springer, 73-107.
19. Newman IA. 2001. Ion transport in roots: measurement of fluxes using ion-selective microelectrodes to characterize transporter function. Plant, Cell and Environment 24, 1-14.
20. Rall W. 1977. Core conductor theory and cable properties of neurons. In: Kandel ER, ed. Handbook of physiology, Vol. 1. Bethesda, MD: American Physiological Society, 39-97.
21. Roberts SK. 2003. TOK homologue in Neurospora crassa: first cloning and functional characterization of an ion channel in a filamentous fungus. Eukaryotic Cell 2, 181-190.
22. Shabala S, Lew RR. 2002. Turgor regulation in osmotically stressed Arabidopsis thaliana epidermal root cells: direct support for the role of inorganic ion uptake as revealed by concurrent flux and cell turgor measurements. Plant Physiology 129, 290-299.
23. Smith PJS, Hammar K, Porterfield DM, Sanger RH, Trimarchi JR. 1999. Self-referencing, non-invasive, ion selective electrode for single cell detection of trans-plasma membrane calcium flux. Microscopy Research and Technique 46, 398-417.
24. Tyerman SD, Beilby M, Whittington J, Juswona U, Newman I, Shabala S. 2001. Oscillations in proton transport revealed from simultaneous measurements of net current and net proton fluxes from isolated protoplasts: MIFE meets patch clamp. Australian Journal of Plant Physiology 28, 591-606.
25. Vogel HJ. 1956. A convenient growth medium for Neurospora. Microbial Genetics Bulletin 13, 42-46.