Variability in anticardiolipin antibody detection: Role of nonspecific IgG binding and different microtiter plates

Clinical and Applied Thrombosis/Hemostasis

Volumn 13, Issue 4, 2007, Pages 404-409

  Pellegrino, Nelly M ^a   Caccavo, Domenico ^a,b  

^a UNIVERSITY OF BARI   (Italy)

^b UNIVERSITY OF BARI MEDICAL SCHOOL   (Italy)

Author keywords

Anticardiolipin antibodies; ELISA; Microtiter plates; Sample blank

Indexed keywords

CARDIOLIPIN; CARDIOLIPIN ANTIBODY; IMMUNOGLOBULIN G; IMMUNOGLOBULIN G ANTIBODY;

ADULT; ANTIBODY DETECTION; ANTIPHOSPHOLIPID SYNDROME; ARTICLE; BINDING AFFINITY; BLOOD SAMPLING; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; LABORATORY APPARATUS, EQUIPMENT AND SUPPLIES; MALE; MICROTITER PLATE ASSAY; OPTICAL DENSITY; PRIORITY JOURNAL; THROMBOCYTOPENIA; THROMBOSIS; BLOOD; CASE CONTROL STUDY; COMPARATIVE STUDY; EVALUATION; IMMUNOLOGY; INSTRUMENTATION; METHODOLOGY; MIDDLE AGED; STANDARD;

ADULT; ANTIBODIES, ANTICARDIOLIPIN; ANTIPHOSPHOLIPID SYNDROME; CASE-CONTROL STUDIES; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMANS; IMMUNOGLOBULIN G; MALE; MIDDLE AGED; REFERENCE STANDARDS;

EID: 35748981806     PISSN: 10760296     EISSN: None     Source Type: Journal    
DOI: 10.1177/1076029607303540     Document Type: Article

References (18)

1. Harris EN Antiphospholipid antibodies. Br J Haematol. 1990 ; 74: 1-9.
2. Wilson WA, Gharavi AE, Koike T., et al. International consensus statement on preliminary classification criteria for definite antiphospholipid syndrome. Arthritis Rheum. 1999 ; 42: 1309-1311.
3. Loizou S., McCrea JD, Rudge AC, et al. Measurement of anti-cardiolipin antibodies by an enzyme-linked immunosorbent assay (ELISA): standardization and quantitation of results. Clin Exp Immunol. 1985 ; 62: 738-745.
4. Reber G., Arvieux J., Comby E., et al. Multicenter evaluation of nine commercial kits for the quantitation of anticardiolipin antibodies. Thromb Haemost. 1995 ; 73: 444-452.
5. Tincani A., Balestrieri G., Allegri F., et al. Overview on anticardiolipin ELISA standardization. J Autoimmun. 2000 ; 15: 195-197.
6. Favaloro EJ, Silvestrini R. Assessing the usefulness of anticardiolipin antibody assays. Am J Clin Pathol. 2002 ; 118: 548-557.
7. Wong Rcw, Favaloro EJ, Pollock W., et al. A multi-centre evaluation of the intra-assay and inter-assay variation of commercial and in-house anti-cardiolipin antibody assays. Pathology. 2004 ; 36: 182-192.
8. Kutteh WH, Franklin RD Assessing the variation in antiphospholipid antibody (APA) assays: comparison of results from 10 centers. Am J Obstet Gynecol. 2004 ; 191: 440-448.
9. Favaloro EJ, Wong Rcw, Silvestrini R., McEvoy R., Jovanovich S., Roberts-Thomson P. A multilaboratory peer assessment quality assurance program-based evaluation of anticardiolipin antibody, and beta2-glycoprotein I antibody testing. Semin Thromb Hemost. 2005 ; 31: 73-84.
10. Tincani A., Allegri F., Sanmarco M., et al. Anticardiolipin antibody assay: a methodological analysis for a better consensus in routine determinations-a cooperative project of the European Antiphospholipid Forum. Thromb Haemost. 2001 ; 86: 575-583.
11. Greaves M., Cohen H., MacHin SJ, Mackie I. Guidelines on the investigation and management of the antiphospholipid syndrome. Br J Haematol. 2000 ; 109: 704-715.
12. Triplett DA Antiphospholipid antibodies. Arch Pathol Lab Med. 2002 ; 126: 1424-1429.
13. Wong Rcw, Adelstein S., Gillis D., Favaloro EJ Development of consensus guidelines for anticardiolipin and lupus anticoagulant testing. Semin Thromb Hemost. 2005 ; 31: 39-48.
14. Tincani A., Allegri F., Balestrieri G., et al. Minimal requirements for antiphospholipid antibodies ELISAs proposed by the European Forum on antiphospholipid antibodies. Thromb Res. 2004 ; 114: 553-558.
15. Cohen J. A coefficient of agreement for nominal scales. Educ Psych Meas. 1960 ; 20: 37-46.
16. Cabral AR, Cabiedes J., Alarcon-Segovia D. Tween 20 detaches cardiolipin from ELISA plates and makes anticardiolipin undetectable regardless of the presence of beta 2-glycoprotein-I. J Immunol Methods. 1994 ; 175: 107-114.
17. Hughes JR, Davies JA, Prentice Crm. The effect of different microtiter plates on the enzyme-linked immunosorbent assay (ELISA) for anticardiolipin antibodies (ACAs). Thromb Res. 1996 ; 84: 217-222.
18. Drouvalakis KA, Buchanan Rrc. Microtiter plate and assay buffer alter detection of antiphosphatidylethanolamine antibodies in lupus anticoagulant positive plasma. Thromb Res. 1999 ; 94: 205-212.