The use of ASB-14 in combination with CHAPS is the best for solubilization of human brain proteins for two-dimensional gel electrophoresis

Briefings in Functional Genomics and Proteomics

Volumn 6, Issue 1, 2007, Pages 70-75

  Martins, Daniel ^a   de Oliveira, Bruno Menezes ^a   Farias, Alessandro dos Santos ^a   Horiuchi, Ricardo Shiniti Oka ^a   Domingues, Cleyton Crepaldi ^a   de Paula, Eneida ^a   Marangoni, Sérgio ^a   Gattaz, Wagner Farid ^b   Dias Neto, Emmanuel ^b,c   Novello, José Camillo ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c UNIVERSITY OF TEXAS   (United States)

Author keywords

ASB; Brain; CHAPS; Human brain; Protein extraction; Two dimensional gel electrophoresis

Indexed keywords

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; AMIDOSULFOBETAIN 14; AMIDOSULFOBETAIN 16; BRAIN PROTEIN; BUFFER; DITHIOTHREITOL; MEMBRANE PROTEIN; REAGENT; THIOUREA; UREA; 3 ((3 CHOLAMIDOPROPYL)DIMETHYLAMMONIUM) 1 PROPANESULFONATE; 3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIUM)-1-PROPANESULFONATE; AMIDOSULFOBETAINE 14; AMIDOSULFOBETAINE-14; BETAINE; CHOLIC ACID DERIVATIVE; DETERGENT; DRUG DERIVATIVE; PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CYTOSOL; FRONTAL CORTEX; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN ISOLATION; TWO DIMENSIONAL GEL ELECTROPHORESIS; BRAIN LEVEL; CHEMISTRY; COMPARATIVE STUDY; EVALUATION; HUMAN; METHODOLOGY; SOLUBILITY;

BETAINE; BRAIN CHEMISTRY; CHOLIC ACIDS; DETERGENTS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; PROTEINS; SOLUBILITY;

EID: 35748964743     PISSN: 14739550     EISSN: 14774062     Source Type: Journal    
DOI: 10.1093/bfgp/elm009     Document Type: Article

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