A simplified method of determining synovial fluid chondroitin sulfate chain length

Osteoarthritis and Cartilage

Volumn 15, Issue 12, 2007, Pages 1443-1445

  Brown, M P ^a   Trumble, T N ^a   Sandy, J D ^b   Merritt, K A ^a  

^a University of Florida College of Veterinary Medicine   (United States)

^b RUSH UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Biomarkers; Chondroitin sulfate chain length; Dimethylmethylene blue; Fluorophore assisted carbohydrate electrophoresis; Horse; Joint injury; Synovial fluid

Indexed keywords

CHONDROITIN SULFATE; DIMETHYLMETHYLENE BLUE; GLYCOSAMINOGLYCAN; METHYLENE BLUE; UNCLASSIFIED DRUG;

AREA UNDER THE CURVE; ARTICLE; BONE INJURY; CARBOHYDRATE ANALYSIS; CARTILAGE INJURY; CONTROLLED STUDY; ELECTROPHORESIS; FLUOROPHORE ASSISTED CARBOHYDRATE ELECTROPHORESIS; GEL FILTRATION CHROMATOGRAPHY; HORSE DISEASE; INTERMETHOD COMPARISON; LIMB INJURY; NONHUMAN; PREDICTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SYNOVIAL FLUID; SYNOVIAL FLUID LEVEL; TREADMILL EXERCISE;

ANIMALS; CHONDROITIN SULFATES; CHROMATOGRAPHY, AGAROSE; ELECTROPHORESIS; HORSE DISEASES; HORSES; JOINT DISEASES; METHYLENE BLUE; SYNOVIAL FLUID;

EID: 35748944104     PISSN: 10634584     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.joca.2007.05.018     Document Type: Article

References (10)

1. Brown M.P., Trumble T.N., Plaas A.H.K., Sandy J.D., Romano M., Hernandez J., et al. Exercise and injury increase chondroitin sulfate chain length and decrease hyaluronan chain length in synovial fluid. Osteoarthritis Cartilage 15 (2007)
2. Brown M.P., West L.A., Merritt K.A., and Plaas A.H. Changes in sulfation patterns of chondroitin sulfate in equine articular cartilage and synovial fluid in response to aging and osteoarthritis. Am J Vet Res 59 (1998) 786-791
3. Bayliss M.T., Osborne D., Woodhouse S., and Davidson C. Sulfation of chondroitin sulfate in human articular cartilage. The effect of age, topographical position, and zone of cartilage on tissue composition. J Biol Chem 274 (1999) 15892-15900
4. Sharif M., Osborne D.J., Meadows K., Woodhouse S.M., Colvin E.M., Shepstone L., et al. The relevance of chondroitin and keratan sulphate markers in normal and arthritic synovial fluid. Br J Rheumatol 35 (1996) 951-957
5. Shinmei M., Miyauchi S., Machida A., and Miyazaki K. Quantitation of chondroitin 4-sulfate and chondroitin 6-sulfate in pathologic joint fluid. Arthritis Rheum 35 (1992) 1304-1308
6. Uesaka S., Nakayama Y., Shirai Y., and Yoshihara K. Serum and synovial fluid levels of chondroitin sulfate in patients with osteoarthritis of the knee joint. J Nippon Med Sch 68 (2001) 165-170
7. Belcher C., Yaqub R., Fawthrop F., Bayliss M., and Doherty M. Synovial fluid chondroitin and keratan sulphate epitopes, glycosaminoglycans, and hyaluronan in arthritic and normal knees. Ann Rheum Dis 56 (1997) 299-307
8. Deutsch A.J., Midura R.J., and Plaas A.H.K. Structure of chondroitin sulfate on aggrecan isolated from bovine tibial and costochondral growth plates. J Orthop Res 13 (1995) 230-239
9. le Maire M., Ghazi A., Moller J.V., and Aggerbeck L.P. The use of gel chromatography for the determination of sizes and relative molecular masses of proteins. Interpretation of calibration curves in terms of gel-pore-size distribution. Biochem J 243 (1987) 399-404
10. Farndale R.W., Sayers C.A., and Barrett A.J. A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures. Connect Tissue Res 9 (1982) 247-248