Improved methods for the preparation of [3H]folate polyglutamates: biosynthesis with Lactobacillus casei and enzymatic synthesis with Escherichia coli folylpolyglutamate synthetase

Analytical Biochemistry

Volumn 371, Issue 2, 2007, Pages 127-134

  Naponelli, Valeria ^a   Hanson, Andrew D ^b   Gregory III, Jesse F ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

Author keywords

Biosynthesis; Enzymatic synthesis; Folate; Folylpolyglutamate synthetase; Polyglutamates; Tritium

Indexed keywords

BIOCHEMISTRY; CHEMICAL DETECTION; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ION EXCHANGE; ORGANIC ACIDS; TRITIUM;

BIOLOGICAL SYNTHESIS; ENZYMATIC SYNTHESIS; FOLATE; FOLATE-BINDING PROTEIN; MULTI-CHANNEL ELECTROCHEMICAL DETECTIONS; POLYGLUTAMATES; RECOMBINANT ESCHERICHIA COLI; SYNTHETASES;

BIOSYNTHESIS;

5 METHYLTETRAHYDROFOLIC ACID; ASCORBIC ACID; BACTERIAL ENZYME; ESCHERICHIA COLI PROTEIN; FOLATE BINDING PROTEIN; FOLIC ACID; FOLINIC ACID; FOLYLPOLYGLUTAMATE SYNTHASE; MERCAPTOETHANOL; PTEROPTIN; RECOMBINANT ENZYME; SEPHADEX;

ARTICLE; BACTERIAL GROWTH; BINDING AFFINITY; BIOSYNTHESIS; CATALYSIS; CELL SUSPENSION; ELECTROCHEMICAL DETECTION; ELUTION; ENZYME CHEMISTRY; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; HEATING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LACTOBACILLUS CASEI; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LACTOBACILLUS CASEI; TETRA;

EID: 35549004021     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.08.026     Document Type: Article

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