Evaluation of an indigenous ELISA for diagnosis of Johne's disease and its comparison with commercial kits

Indian Journal of Microbiology

Volumn 47, Issue 3, 2007, Pages 251-258

  Singh, S V ^a   Singh, A V ^a   Singh, P K ^a   Sohal, J S ^a   Singh, N P ^a  

^a CENTRAL INSTITUTE FOR RESEARCH ON GOATS   (India)

Author keywords

Diagnosis; ELISA kit; Fecal culture; Johne's disease; Mycobacterium avium subsp. paratuberculosis; Protoplasmic antigen

Indexed keywords

ANIMALIA; BACILLI (CLASS); BISON; CAPRA HIRCUS; MYCOBACTERIUM AVIUM; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; OVIS ARIES;

EID: 35549002809     PISSN: 00468991     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12088-007-0046-2     Document Type: Article

References (28)

1. Manning EJB & Collins MT (2001) Mycobacterium avium subsp. paratuberculosis: pathogen, pathogenesis and diagnosis. Rev Sci Tech Off Int Epiz 20:133-150
2. Singh SV & Singh N (2000) Effect of climate on Johne's disease incidence in goatherds. Ind J Anim Sci 70: 492-493
3. Goswami TK, Tewari V, Gupta A, Mall R & Ram GC (2000) Sero-prevalence of Mycobacterium paratuberculosis in an organized Pashmina goat farm. Ind J Comp Microbiol Immunol Infect Dis 21:132-135
4. Kumar P, Singh SV, Bhatiya AK, Sevilla I, Singh AV, Whittington RJ, Juste RA, Gupta VK, Singh PK, Sohal JS & Vihan VS (2007) Juvenile Capri-Paratuberculosis (JCP) in India: Incidence and characterization by six diagnostic tests. Small Rumin. Res. (2006) doi:10.1016/j.smallrumres.2006. 10.023, In press.
5. Vihan VS, Singh N & Singh SV (1989) Preliminary observations on fecal culture test for the detection of Mycobacterium paratuberculosis in goats. Ind J Anim Sci 59: 1522-1524
6. Singh N, Singh SV, Gupta VK, Sharma VD, Sharma RK & Katoch VM (1996) Isolation and identification of Mycobacterium paratuberculosis from naturally infected goatherds in India. Ind J Vet Pathol 20:104-108
7. Kumar V (2002) Identification of Mycobacterium avium subsp paratuberculosis in sheep and goats and prospective evaluation of an ELISA and AGID test for the diagnosis of Johne's Disease. Veterinary Microbiology & Virology, College of Veterinary Sciences, Mathura (UP) submitted to CSA University, Kanpur.
8. Collins MT, Wells SJ, Petrini KR, Collins JE, Schultz RD & Whitlock RH (2005) Evaluation of five-antibody detection tests for diagnosis of bovine paratuberculosis. Clin Diagn Lab Immunol 12:685-692
9. Whitlock RH & Rosenberger AE (1990) Fecal culture protocol for Mycobacterium paratuberculosis a recommended procedure. In: Proc. Annual Meeting U.S. Animal Health Association 94:280-285
10. Merkal RS (1984) Paratuberculosis: advances in cultural, serologic and vaccination methods. J Am Vet Med Assoc 184:939-943
11. Merkal RS & Curran BJ (1974). Growth and metabolic characteristics of mycobacterium paratuberculosis. Appl. Microbiol 28:276-279
12. Whittington RJ (2002) Personal communication
13. Sevilla I, Singh SV, Garrido JM, Aduriz, G, Rodriguez S, Geijo MV, Whittington RJ, Saunders V, Whitlock RH & Juste RA (2005) Molecular typing of Paratuberculosis strains from different hosts and regions. Rev Sci Tech Off Int Epiz 24:1061-1066
14. Lowry OH, Rosebrough NJ, Farr AL & Randall RJ (1951) Protein measurement with Folin-phenol reagent. J Biol Chem 193:265-276
15. Milner AR, Lepper AWD, Symonds WN & Gruner E (1987) Analysis by ELISA and Western blotting of antibody reactivities in cattle infected with Mycobacterium paratuberculosis after absorption of serum with Mxxx.phlei. Res Vet Sci 42: 140-144
16. Singh SV, Singh AV, Singh PK, Gupta VK, Kumar S & Vohra J (2007) Sero-prevalence of paratuberculosis in young kids using "Bison type', Mycobacterium avium subsp. paratuberculosis antigen in plate ELISA. Small Rumin Res. 70: 89-92
17. Collins MT (2002) Interpretation of a commercial bovine paratuberculosis Enzyme linked immunosorbent assay by using likelihood ratio. Clin Diagn Lab Immunol 9: 1367-1371
18. Arizmendi F & Grimes JE (1995) Comparison of Gimenez staining method and antigen detection ELISA with culture for detecting Chlamydia in bird. J Vet Diagn Invest 7: 400-401
19. Landis RJ & Koch GG (1977) The measurement of observer agreement for categorical data. Biometrics 33: 159-174
20. Corpa JM, Perez V & Marin JFG (2000) Differences in the immune responses in lambs and kids vaccinated against paratuberculosis, according to the age of vaccination. Vet Microbiol 77:475-485
21. Singh SV, Singh AV, Singh R, Sandhu KS, Gupta VK & Misra S (2005) Survey of ruminant population of Northern India for the incidence of Mycobacterium avium subspecies paratuberculosis infection. In: Neilsen, S.S. (eds) 8th ICP. Copenhagen, Denmark. p 60
22. Salgado M, Manning EJB & Collins MT (2005) Performance of a Johne's disease enzyme linked immunosorbent assay adapted for milk samples from goats. J Vet Diagn Invest 17:350-354
23. Tripathi BN & Parihar NS (1999) Status of paratuberculosis in goats: A 5 years study based on post-mortem cases. Indian J Vet Pathol 23:79-80
24. Singh SV & Vihan VS (2004) Detection of Mycobacterium avium subsp. paratuberculosis in goat milk. Small Rumin Res 54:231-235
25. Singh N, Vihan VS, Singh SV & Gupta VK (1998) Prevalence of Johne's disease in organized goatherds. Indian J Anim Sci 68:41-42
26. Gupta VK, Singh N, Singh SV & Shankar H (1996) Faecal microscopic examination - A tool for diagnosis of Johne's disease in small ruminants. Indian Vet Med J 20:213-214
27. Singh SV & Vihan VS. (2004). Incidence of Mycobacterium avium subsp. paratuberculosis in clinically suspected small ruminants. The Indian J. Small Rumin 9: 56-59.
28. Singh SV, Singh PP, Singh N & Gupta VK (2000) Characterization of lipid pattern of M. paratuberculosis isolated from goats and sheep. Indian J. Ani Sci 70:899-903