Structural insights into the recognition of substrates and activators by the OSR1 kinase

EMBO Reports

Volumn 8, Issue 9, 2007, Pages 839-845

  Villa, Fabrizio ^a   Goebel, Jürgen ^a   Rafiqi, Fatema H ^a   Deak, Maria ^a   Thastrup, Jacob ^a   Alessi, Dario R ^a   van Aalten, Daan M F ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; OXIDATIVE STRESS RESPONSIVE KINASE 1; PHENYLALANINE; PHOSPHOTRANSFERASE; PROTEIN KINASE; PROTEIN KINASE WNK1; PROTEIN KINASE WNK4; SERINE; SODIUM POTASSIUM CHLORIDE COTRANSPORTER; SPS1 RELATED PROLINE ALANINE RICH KINASE; THREONINE; UNCLASSIFIED DRUG; VALINE;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME SUBSTRATE; HUMAN; HUMAN CELL; MOUSE; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; RAT; SEQUENCE ALIGNMENT; STRUCTURAL PROTEOMICS; STRUCTURE ANALYSIS;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; CONSERVED SEQUENCE; CRYSTALLOGRAPHY, X-RAY; ENZYME ACTIVATORS; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; PEPTIDES; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

EID: 35548971082     PISSN: 1469221X     EISSN: 14693178     Source Type: Journal    
DOI: 10.1038/sj.embor.7401048     Document Type: Article

References (22)

1. +, 2Cl-cotransporter in HeLa cells. Proc Natl Acad Sci USA 103: 10883-10888
2. Cowtan K, Main P (1998) Miscellaneous algorithms for density modification. Acta Crystallogr D 54: 487-493
3. Delpire E, Gagnon KB (2006) Genome-wide analysis of SPAK/OSR1 binding motifs. Physiol Genomics 28: 223-231
4. Dowd BF, Forbush B (2003) PASK (proline-alanine-rich STE20-related kinase), a regulatory kinase of the Na-K-Cl cotransporter (NKCC1). J Biol Chem 278:27347-27353
5. Egloff MP, Johnson DF, Moorhead G, Cohen PT, Cohen P, Barford D (1997) Structural basis for the recognition of regulatory subunits by the catalytic subunit of protein phosphatase 1. EMBO J 16: 1876-1887
6. Emsley P, Cowtan K (2004) Coot: Model-building tools for molecular graphics. Acta Crystallogr D 60: 2126-2132
7. Gagnon KB, England R, Delpire E (2006) Volume sensitivity of cation-Cl-cotransporters is modulated by the interaction of two kinases: Ste20-related proline-alanine-rich kinase and WNK4. Am J Physiol Cell Physiol 290:C134-C142
8. Holm L, Sander C (1993) Protein structure comparison by alignment of distance matrices. J Mol Biol 233: 123-138
9. Hubbard MJ, Cohen P (1993) On target with a new mechanism for the regulation of protein phosphorylation. Trends Biochem Sci 18 172-177
10. Johnson DF, Moorhead G, Caudwell FB, Cohen P, Chen YH, Chen MX, Cohen PT (1996) Identification of protein-phosphatase-1-binding domains on the glycogen and myofibrillar targeting subunits. Eur J Biochem 239 317-325
11. Meiselbach H, Sticht H, Enz R (2006) Structural analysis of the protein phosphatase 1 docking motif: Molecular description of binding specifities identifies interacting proteins. Chem Biol 13: 49-59
12. Moriguchi T, Urushiyama S, Hisamoto N, Iemura S, Uchida S, Natsume T, Matsumoto K, Shibuya H (2005) WNK1 regulates phosphorylation of cation-chloride-coupled cotransporters via the STE20-related kinases, SPAK and OSR1. J Biol Chem 280: 42685-42693
13. Murshudov GN, Vagin AA, Dodson EJ (1997) Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D 53: 240-255
14. Pape T, Schneider TR (2004) HKL2MAP: A graphical user interface for macromolecular phasing with SHELX. J Appl Crystallogr 37: 843-844
15. Perrakis A, Morris R, Lamzin VS (1999) Automated protein model building combined with iterative structure refinement. Nat Struct Biol 6 458-463
16. +-2Cl-cotransporter in the nervous system: Evidence for a scaffolding role of the kinase. J Biol Chem 278: 52848-52856
17. Sheldrick GM, Schneider TR (1997) SHELXL: High resolution refinement. Methods Enzymol 277: 319-343
18. Vagin A, Teplyakov A (1997) MOLREP: An automated program for molecular replacement. J Appl Crystallogr 30: 1022-1025
19. Vitari AC, Deak M, Morrice NA, Alessi DR (2005) The WNK1 and WNK4 protein kinases that are mutated in Gordon's hypertension syndrome phosphorylate and activate SPAK and OSR1 protein kinases. Biochem J 391: 17-24
20. Vitari AC, Thastrup J, Rafiqi FH, Deak M, Morrice NA, Karlsson HK, Alessi DR (2006) Functional interactions of the SPAK/OSR1 kinases with their upstream activator WNK1 and downstream substrate NKCC1. Biochem J 397:223-231
21. Wilson FH et al (2001) Human hypertension caused by mutations in WNK kinases. Science 293: 1107-1112
22. Zagórska A et al (2007) Regulation of activity and localisation of the WNK1 protein kinase by hyperosmotic stress. J Cell Biol 176: 89-100