Microfluidic device for rapid detection of cytomegalovirus (CMV) by sequence-specific hybridization of PCR-amplified CMV-DNA

Proceedings of 2006 International Conference on Microtechnologies in Medicine and Biology

Volumn , Issue , 2006, Pages 209-212

  Briones, Maria Portia ^a   Yamashita, Kenichi ^a   Numata, Sanae ^b   Miyazaki, Masaya ^a,c   Nakamura, Yasuhiro ^b   Maeda, Hideaki ^a,c  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b ST MARY S HOSPITAL   (Japan)

^c KYUSHU UNIVERSITY   (Japan)

Author keywords

Cytomegalovirus; DNA hybridization; Laminar flow; Microfluidic device; PCR

Indexed keywords

DNA; FLUORESCENCE MICROSCOPY; LAMINAR FLOW; MICROCHANNELS; MICROFLUIDICS; MICROPROCESSOR CHIPS; PEPTIDES; VIRUSES;

ANTIGENEMIA TESTS; CYTOMEGALOVIRUS; LOW LEVEL VIREMIA; MICROFLUIDIC DEVICES; PEPTIDE NUCLEIC ACID (PNA);

FLUIDIC DEVICES;

EID: 35548952824     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1109/MMB.2006.251530     Document Type: Conference Paper

References (8)

1. W.L. Drew. "Cytomegalovirus infection in patients with AIDS." Clin Infect Dis. 14:608-615, 1992.
2. D. Tarrago, M.L. Mateos, A. Avellon, M.D. Perez-Vazquez, A. Tenorio. "Quantitation of cytomegalovirus DNA in cerebrospinal fluid and serum specimens from AIDS patients using a novel highly sensitive nested competitive PCR and the cobas amplicor CMV monitor." J Med Virol. 72: 249-56, 2004.
3. P. Grossi, L. Minoli, E. Percivalle, W. Irish, M. Vigano, G. Gema."Clinical and virological monitoring of human cytomegalovirus infection in 294 heart transplant recipients." Transplantation 59: 847-851, 1995.
4. C. Muheim, G. Vogel, C. Seydoux, M. Gillet, F. Mosimann, L. Von Segesser, et al. "Determinants of protracted cytomegalovirus infection in solid-organ transplant patients". Transplantation. 74: 226-36, 2002.
5. A. Y. Yamamoto, M.M. Mussi-Pinhata, P. Cristina, G. Pinto, L.T. Moraes Figueredo, S.M. Jorge. "Congenital cytomegalovirus infection in preterm and full-term newborn infants from a population with a high seroprevalence rate". Pediatr Infect Dis J. 20:188-192, 2001.
6. R. Delgado, C. Lumbreras, C. Alba,M.A. Pedraza, J. R. Otero, R. Gomez, E. Moreno,A. R. Noriega, and C.V. Paya. "Low predictive value of polymerase chanin reaction for diagnosis of cytomegalovirus disease in liver transplants recipients."J. Clin. Microbiol. 30:1876-1878., 1992.
7. H. Piiparinen, K. Hockerstedt, C. Gronhagen-Riska, I. Lautenschlager. "Comparison of two quantitative CMV Monitor and Taqman Assay, and pp65-antigenemia assay in the determination of viral loads from peripheral blood of organ transplant patients". J Clin Vir 30:258-266, 2004.
8. K. Yamashita, Y. Yamaguchi, M. Miyazaki, H. Nakamura, H. Shimizu, H.. Maeda. "Sequence-selective DNA detection using multiple laminar streams: A novel microfluidic analysis method". Lab. Chip 4:1-3, 2004.