Digital fluorescence microscopy of cell cytoplasts with and without the centrosome

Methods in Cell Biology

Volumn 67, Issue 67, 2001, Pages 43-51

  Rodionov, Vladimir ^a   Nadezhdina, Elena ^b   Peloquin, John ^c   Borisy, Gary ^c  

^a UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

^b INSTITUTE OF PROTEIN RESEARCH   (Russian Federation)

^c NORTHWESTERN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTINEOPLASTIC AGENT; COMPLEMENTARY DNA; CYTOCHALASIN; NOCODAZOLE;

ANIMAL; ARTICLE; CELL FRACTIONATION; CENTRIFUGATION; CENTROSOME; CHEMISTRY; CYTOPLASM; FLUORESCENCE MICROSCOPY; IMAGE PROCESSING; METABOLISM; METHODOLOGY; SWINE; ULTRASTRUCTURE;

ANIMALS; ANTINEOPLASTIC AGENTS; CELL FRACTIONATION; CENTRIFUGATION; CENTROSOME; CYTOCHALASINS; CYTOPLASM; DNA, COMPLEMENTARY; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, FLUORESCENCE; NOCODAZOLE; SWINE;

EID: 35448941572     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s0091-679x(01)67004-3     Document Type: Article

References (14)

1. Borisy, G. G., Marcum, J. M., Olmsted, J. B., Murphy, D. B., and Johnson, K. A. (1975). Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro. Ann. N.Y.Acad. Sci. 253, 107-132.
2. Goldman, R. D., Pollack, R., and Hopkins, N. (1973). Preservation of normal behavior by enucleated cells in culture. Proc. Natl. Acad. Sci. USA 70, 750-754.
3. Graessmann, M., and Graessmann, A. (1976). Early simian virus-40 RNA contains information for tumor antigen formation and chromatin replication. Proc. Natl. Acad. Sci USA 73, 366-370.
4. Karsenti, E., Kobayashi, S., Mitchison, T., and Kirschner, M. (1984). Role of the centrosome in organizing the interphase microtubule array: Properties of cytoplasts containing or lacking centrosomes. J. Cell Biol. 98, 1763-1766.
5. Khodjakov, A., and Rieder, C. L. (1999). The sudden recruitment of gamma-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules. J. Cell Biol. 146, 585-596.
6. Piel, M., Meyer, P., Khodjakov, A., Rieder, C. L., and Bornens, M. (2000). The respective contributions of the mother and daughter centrioles to centrosome activity and behavior in vertebrate cells. J. Cell Biol. 149, 317-330.
7. Pollack, R., Goldman, R. D., Conlon, S., and Chang, C. (1974). Properties of enucleated cells. II. Characteristic overlapping of transformed cells is reestablished by enucleates. Cell 3, 51-54.
8. Prescott, D. M., Myerson, D., and Wallace, J. (1972). Enucleation of mammalian cells with cytochalasin B. Exp. Cell Res. 71, 480-485.
9. Rodionov, V. I., Nadezhdina, E., and Borisy, G. G. (1999). Centrosomal control of microtubule dynamics. Proc. Natl. Acad. Sci. USA 96, 115-120.
10. Shay, J. W., Porter, K. R., and Prescott, D. M. (1974). The surface morphology and fine structure of CHO (Chinese hamster ovary) cells following enucleation. Proc. Natl. Acad. Sci. USA 71, 3059-3063.
11. Weingarten, M. D., Lockwood, A. H., Hwo, S. Y., and Kirschner, M. W. (1975). A protein factor essential for microtubule assembly. Proc. Natl. Acad. Sci. USA 72, 1858-1862.
12. Wessendorf, M. W, and Brelje, T. C. (1992). Which fluorophore is the brightest? A comparison of the staining obtained using fluorescein, tetramethylrhodamine, lissamine rhodamine, texas red, and cyanine 3.18. Histochemistry 98, 81-85.
13. Wigler, M. H., Neugut, A. I., and Weinstein, I. B. (1976). Enucleation of mammalian cells in suspension. Methods Cell Biol. 14, 87-93.
14. Zhai, Y., and Borisy, G. G. (1994). Quantitative determination of the proportion of microtubule polymer present during the mitosis-interphase transition. J. Cell Sci. 107, 881-890.