Functional expression of hexahistidine-tagged β-subunit of yeast F1-ATPase and isolation of the enzyme by immobilized metal affinity chromatography

Protein Expression and Purification

Volumn 37, Issue 1, 2004, Pages 97-101

  Ichikawa, Naoki ^a   Mizuno, Miki ^a  

^a Osaka Prefecture University   (Japan)

Author keywords

ATP synthase

Indexed keywords

ATP2 PROTEIN, S CEREVISIAE; ATPASE INHIBITORY PROTEIN; ENZYME INHIBITOR; HISTIDINE; METAL; PROTEIN; PROTEIN SUBUNIT; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATASE; SACCHAROMYCES CEREVISIAE PROTEIN; SIGNAL PEPTIDE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CYTOLOGY; DRUG ANTAGONISM; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MITOCHONDRION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; ENZYME INHIBITORS; HISTIDINE; METALS; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; PROTEIN SORTING SIGNALS; PROTEIN SUBUNITS; PROTEINS; PROTON-TRANSLOCATING ATPASES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 3543125431     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.06.002     Document Type: Article

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