Expression of the melittin gene of Apis cerana cerana in Escherichia coli

Protein Expression and Purification

Volumn 37, Issue 1, 2004, Pages 213-219

  Shi, Wan Jun ^a   Xu, Hai Jun ^a   Cheng, Jia An ^a   Zhang, Chuan Xi ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Apis cerana cerana; Melittin; Protein expression

Indexed keywords

HYBRID PROTEIN; MELITTIN;

ANIMAL; ARTICLE; BEE; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; RABBIT;

ANIMALS; BEES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; HUMANS; MELITTEN; RABBITS; RECOMBINANT FUSION PROTEINS;

APIS; APIS CERANA CERANA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS; VECTORS;

EID: 3543099821     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.05.010     Document Type: Article

References (13)

1. Akashi S., Takio K. Structure of melittin bound to phospholipid micelles studied using hydrogen-deuterium exchange and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. American Society for Mass Spectrometry. 12:2001;1247-1253
2. Habermann E. Bee and wasp venoms. Science. 177:1972;314-322
3. Lazarev V.N., Govorun V.M., Parfenova T.M., Akopian T.A., Lopukhin Y.M. Effect of controlled expression of the melittin gene on infection caused by Mycoplasma hominis in cell culture. Doklady Biochemistry and Biophysics. 378:2001;186-187
4. Lazarev V.N., Parfenova T.M., Gularyan S.K., Misyurina O.Y., Akopian T.A., Govorun V.M. Induced expression of melittin, an antimicrobial peptide, inhibits infection by Chlamydia trachomatis and Mycoplasma hominis in a HeLa cell line. International Journal of Antimicrobial Agents. 19:2002;133-137
5. Liu P., Davis P., Liu H., Krishnan T.R. Evaluation of cytotoxicity and absorption enhancing effects of melittin - a novel absorption enhancer. European Journal of Pharmaceutics and Biopharmaceutics. 48:1999;85-87
6. Saini S.S., Chopra A.K., Peterson J.W. Melittin activates endogenous phospholipase D during cytolysis of human monocytic leukemia cells. Toxicon. 37:1999;1605-1619
7. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. second ed.:1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
8. Shi W.J., Zhang S.F., Zhang C.X., Cheng J.A. Cloning and sequencing of cDNA encoding prepromelittin in Apis cerana cerana and Apis mellifera. Acta Entomologica Sinica. 46:2003;254-258
9. Vlask R., Unger-ullmann C., Kreil G., Frischauf A.-M. Nucleotide sequence of cloned cDNA coding for honeybee prepromelittin. European Journal of Biochemistry. 135:1983;123-126
10. Wang G.L., Li D.L., Fang H.J. Site-directed mutagenesis of melittin gene and its expression in Escherichia coli. Acta Genetica Sinica. 27(2):2000;176-182
11. Wang G.L., Li D.L., Fang H.J. Cloning of promelittin cDNA and its expression in Escherichia coli. Acta Microbiologica Sinica. 41:2001;181-185
12. Winder D., Gunzburg W.H., Erfle V., Salmons B. Expression of antimicrobial peptides has an antitumour effect in human cells. Biochem. Biophys. Res. Commun. 242:1998;608-612
13. Yu X.D., Xiong Y.L. The activation of melittin from Apis mellifera venom to rabbit platelets. Journal of Chongqing Teachers College (Natural Science Edition). 13(1):1996;15-20