Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis

Brazilian Journal of Medical and Biological Research

Volumn 37, Issue 7, 2004, Pages 949-955

  Sato, N S ^a   Suzuki, T ^b   Ueda, T ^b   Watanabe, K ^b   Hirata, R D C ^c   Hirata, M H ^c  

^a INSTITUTO ADOLFO LUTZ   (Brazil)

^b UNIVERSITY OF TOKYO   (Japan)

^c UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Cloning; GST; Recombinant antigen; Syphilis; Treponema pallidum

Indexed keywords

ANTIGENICITY; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST; DNA SEQUENCE; ESCHERICHIA COLI; GENE OVEREXPRESSION; HUMAN; IMMUNOBLOTTING; IMMUNOREACTIVITY; LABORATORY DIAGNOSIS; LEPTOSPIROSIS; LIQUID CHROMATOGRAPHY; LYME DISEASE; MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PEPTIDE MAPPING; POLYACRYLAMIDE GEL ELECTROPHORESIS; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS; SYPHILIS; SYPHILIS SEROLOGY; TREPONEMA PALLIDUM;

ESCHERICHIA COLI; TREPONEMA; TREPONEMA PALLIDUM; TREPONEMA PALLIDUM SUBSP. PALLIDUM STR. NICHOLS;

EID: 3543049608     PISSN: 0100879X     EISSN: 1414431X     Source Type: Journal    
DOI: 10.1590/S0100-879X2004000700002     Document Type: Article

References (20)

1. Fieldsteel AH, Cox DL & Moeckli RA (1981). Cultivation of virulent Treponema pallidum in tissue culture. Infection and Immunity, 32: 908-915.
2. Norris SJ & Stell S (1994). Antigenic complexity of Treponemas pallidum: antigenicity and surface localization of major polypeptides. Journal of Immunology, 133: 2686-2692.
3. Hanff PA, Bishop NH, Miller JN & Lovett MA (1983). Humoral response in experimental syphilis to polypeptides of Treponema pallidum. Journal of Immunology, 131: 1973-1977.
4. Byrne RE, Laska S, Bell M, Larson D, Philips J & Todd J (1992). Evaluation of a Treponema pallidum Western immunoblot assay as a confirmatory test for syphilis. Journal of Clinical Microbiology, 30: 115-122.
5. Hanff PA, Fehninger TE, Miller JN & Lovett MA (1982). Humoral immune response in human syphilis to polypeptides of Treponema pallidum. Journal of Immunology, 129: 1287-1291.
6. Akins DR, Purcell BK, Mitra MM, Norgard MV & Radolf JD (1993). Lipid modification of the 17-kilodalton membrane immunogen of Treponema pallidum determines macrophage activation as well as amphiphilicity. Infection and Immunity, 61: 1202-1210.
7. Chamberlain NR, Brandt ME, Erwuin AL, Radolf JD & Norgard MV (1989). Major integral membrane protein immunogens of Treponema pallidum are proteolipids. Infection and Immunity, 57: 2872-2877.
8. Norgard MV, Chamberlain NR, Swancutt MA & Goldberg MS (1986). Cloning and expression of the major 47-kilodalton surface immunogen of Treponema pallidum in Escherichia coli. Infection and Immunity, 54: 500-506.
9. Weigel LM, Brandt ME & Norgard MV (1992). Analysis of the N-terminal region of the 47-kilodalton integral membrane lipoprotein of Treponema pallidum. Infection and Immunity, 60: 1568-1576.
10. Centurion-Lara A, Arrool T, Castilho R, Shaffer JM, Castro C, van Voorhis WC & Lukehart SA (1997). Conservation of the 15-kilodalton lipoprotein among Treponema pallidum subspecies and strains and other pathogenic treponemes: genetic and antigenic analyses. Infection and Immunity, 65: 1440-1444.
11. Purcell BK, Chamberlain NR, Goldberg MS, Andrews LP, Robinson EJ, Norgard MV & Radolf JD (1989). Molecular cloning and characterization of the 15-kilodalton major immunogen of Treponema pallidum. Infection and Immunity, 57: 3708-3714.
12. Sato NS (1999). Clonagem, expressão e caracterização de antígenos recombinantes do Treponema pallidum com potencial imunodiagnóstico. Doctoral thesis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.
13. Sato NS, Hirata MH, Hirata RDC, Zerbini LCMS, Silveira EPR, Melo CS & Ueda M (1999). Analysis of Treponema pallidum recombinant antigens for diagnosis of syphilis by Western blotting technique. Revista do Instituto de Medicina Tropical de São Paulo, 41: 115-118.
14. Brinkmann U, Mattes RE & Buckel P (1989). High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product. Gene, 85: 91-114.
15. Grieco F, Hay JM & Hull RI (1992). An improved procedure for the purification of protein fused with glutathione S-transferase. BioTechniques, 13: 856-858.
16. Fujimura K, Ise N, Ueno E, Hori T, Fujii N & Okada M (1997). Reactivity of recombinant Treponema pallidum (r-Tp) antigens with anti-Tp antibodies in human syphilitic sera evaluated by ELISA. Journal of Clinical and Laboratory Analysis, 11: 315-322.
17. Lefevre JC, Bertrand MA, Bauriaud R & Lareng MB (1992). False positive reactions occurring with Captia Syphilis G EIA in sera from patients with Lyme disease. Genitourinary Medicine, 68: 142 (letter).
18. Radolf JD, Chamberlain NL, Clausell A & Norgard MV (1988). Identification and localization of integral membrane proteins of virulent Treponema pallidum subsp. pallidum by phase partitioning with nonionic detergent Triton X-114. Infection and Immunity, 56: 490-498.
19. Belisle JT, Brandt ME, Radolf JD & Norgard MV (1994). Fatty acids of Treponema pallidum and Borrelia burgdorferi lipoproteins. Journal of Bacteriology, 176: 2151-2157.
20. Brandt ME, Riley BS, Radolf JD & Norgard MV (1990). Immunogenic integral membrane proteins of Borrelia burgdorferi are lipoproteins. Infection and Immunity, 58: 983-991.