Modulation of multidrug resistance-associated protein 2 (Mrp2) and Mrp3 expression and function with small interfering RNA in sandwich-cultured rat hepatocytes

Molecular Pharmacology

Volumn 66, Issue 4, 2004, Pages 1004-1010

  Tian, Xianbin ^a   Zamek Gliszczynski, Maciej J ^a   Zhang, Peijin ^a,b   Brouwer, Kim L R ^a,c  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b Qualyst Inc   (United States)

^c University of North Carolina   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; FLUORESCENT DYE; LUCIFERASE; MULTIDRUG RESISTANCE PROTEIN 2; MULTIDRUG RESISTANCE PROTEIN 3; RADIXIN; SMALL INTERFERING RNA;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BILIARY EXCRETION; CONTROLLED STUDY; DRUG DELIVERY SYSTEM; DRUG EFFICACY; ENZYME ACTIVITY; ENZYME IMMUNOASSAY; HUMAN; HUMAN CELL; INTRAHEPATIC BILE DUCT; LIVER CELL CULTURE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; RAT; RNA INTERFERENCE;

ANIMALS; GENE EXPRESSION; HEPATOCYTES; MEMBRANE TRANSPORT PROTEINS; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; RATS; RATS, WISTAR; RNA, SMALL INTERFERING;

EID: 3543003840     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.66.4.     Document Type: Article

References (39)

1. Akita H, Suzuki H, and Sugiyama Y (2001) Sinusoidal efflux of taurocholate is enhanced in Mrp2-deficient rat liver. Pharm Res (NY) 18:1119-1125.
2. Allen JD, Brinkhuis RF, Wijnholds J, and Schinkel AH (1999) The mouse Bcrp1/Mxr/Abcp gene: amplification and overexpression in cell lines selected for resistance to topotecan, mitoxantrone, or doxorubicin. Cancer Res 59:4237-4241.
3. Annaert PP, Turncliff RZ, Booth CL, Thakker DR, and Brouwer KL (2001) P-glycoprotein-mediated in vitro biliary excretion in sandwich-cultured rat hepatocytes. Drug Metab Dispos 29:1277-1283.
4. Buchler M, Konig J, Brom M, Kartenbeck J, Spring H, Horie T, and Keppler D (1996) cDNA cloning of the hepatocyte canalicular isoform of the multidrug resistance protein, cMrp, reveals a novel conjugate export pump deficient in hyperbilirubinemic mutant rats. J Biol Chem 271:15091-15098.
5. Cabaud PG and Wroblewski F (1958) Colorimetric measurement of lactic dehydrogenase activity of body fluids. Am J Clin Pathol 30:234-236.
6. Cattori V, van Montfoort JE, Stieger B, Landmann L, Meijer DK, Winterhalter KH, Meier PJ, and Hagenbuch B (2001) Localization of organic anion transporting polypeptide 4 (Oatp4) in rat liver and comparison of its substrate specificity with Oatpl, Oatp2 and Oatp3. Pflueg Arch Eur J Physiol 443:188-195.
7. Cioca DP, Aoki Y, and Kiyosawa K (2003) RNA interference is a functional pathway with therapeutic potential in human myeloid leukemia cell lines. Cancer Gene Ther 10:125-133.
8. Cui Y, Konig J, and Keppler D (2001) Vectorial transport by double-transfected cells expressing the human uptake transporter SLC21A8 and the apical export pump ABCC2. Mol Pharmacol 60:934-943.
9. Donze O and Picard D (2002) RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase. Nucleic Acids Res 30:e46.
10. Dykxhoorn DM, Novina CD, and Sharp PA (2003) Killing the messenger: short RNAs that silence gene expression. Nat Rev Mol Cell Biol 4:457-467.
11. Germann UA, Willingham MC, Pastan I, and Gottesman MM (1990) Expression of the human multidrug transporter in insect cells by a recombinant baculovirus. Biochemistry 29:2295-2303.
12. Hannon GJ (2002) RNA interference. Nature (Lond) 418:244-251.
13. Hirohashi T, Suzuki H, and Sugiyama Y (1999) Characterization of the transport properties of cloned rat multidrug resistance-associated protein 3 (MRP3). J Biol Chem 274:15181-15185.
14. Keitel V, Kartenbeck J, Nies AT, Spring H, Brom M, and Keppler D (2000) Impaired protein maturation of the conjugate export pump multidrug resistance protein 2 as a consequence of a deletion mutation in Dubin-Johnson syndrome. Hepatology 32:1317-1328.
15. Kikuchi S, Hata M, Fukumoto K, Yamane Y, Matsui T, Tamura A, Yonemura S, Yamagishi H, Keppler D, and Tsukita S (2002) Radixin deficiency causes conjugated hyperbilirubinemia with loss of Mrp2 from bile canalicular membranes. Nat Genet 31:320-325.
16. Kocher O, Comella N, Gilchrist A, Pal R, Tognazzi K, Brown LF, and Knoll JH (1999) PDZK1, a novel PDZ domain-containing protein up-regulated in carcinomas and mapped to chromosome 1q21, interacts with cMOAT (MRP2), the multidrug resistance-associated protein. Lab Investig 79:1161-1170.
17. Kojima H, Nies AT, Konig J, Hagmann W, Spring H, Uemura M, Fukui H, and Keppler D (2003) Changes in the expression and localization of hepatocellular transporters and radixin in primary biliary cirrhosis. J Hepatol 39:693-702.
18. Konishi M, Wu CH, and Wu GY (2003) Inhibition of HBV replication by siRNA in a stable HBV-producing cell line. Hepatology 38:842-850.
19. Kool M, van der Linden M, de Haas M, Scheffer GL, de Vree JM, Smith AJ, Jansen G, Peters GJ, Ponne N, Scheper RJ, et al. (1999) MRP3, an organic anion transporter able to transport anti-cancer drugs. Proc Natl Acad Sci USA 96:6914-6919.
20. Krichevsky AM and Kosik KS (2002) RNAi functions in cultured mammalian neurons. Proc Natl Acad Sci USA 99:11926-11929.
21. Kruh GD and Belinsky MG (2003) The MRP family of drug efflux pumps. Oncogene 22:7537-7552.
22. LeCluyse EL, Audus KL, and Hochman JH (1994) Formation of extensive canalicular networks by rat hepatocytes cultured in collagen-sandwich configuration. Am J Physiol 266:C1764-C1774.
23. Liu X, Chism JP, LeCluyse EL, Brouwer KE, and Brouwer KL (1999a) Correlation of biliary excretion in sandwich-cultured rat hepatocytes and in vivo in rats. Drug Metab Dispos 27:637-644.
24. Liu X, LeCluyse EL, Brouwer KR, Gan LS, Lemasters JJ, Stieger B, Meier PJ, and Brouwer KL (1999b) Biliary excretion in primary rat hepatocytes cultured in a collagen-sandwich configuration. Am J Physiol 277:G12-G21.
25. 2+ modulation to evaluate biliary excretion in sandwich-cultured rat hepatocytes. J Pharmacol Exp Ther 289:1592-1599.
26. Luttringer O, Theil FP, Lave T, Wernli-Kuratli K, Guentert TW, and de Saizieu A (2002) Influence of isolation procedure, extracellular matrix and dexamethasone on the regulation of membrane transporters gene expression in rat hepatocytes. Biochem Pharmacol 64:1637-1650.
27. Miyagishi M and Taira K (2002) U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat Biotechnol 20:497-500.
28. Mottino AD, Hoffman T, Jennes L, and Vore M (2000) Expression and localization of multidrug resistant protein mrp2 in rat small intestine. J Pharmacol Exp Ther 293:717-723.
29. Nies AT, Konig J, Pfannschmidt M, Klar E, Hofmann WJ, and Keppler D (2001) Expression of the multidrug resistance proteins MRP2 and MRP3 in human hepatocellular carcinoma. Int J Cancer 94:492-499.
30. Nieth C, Priebsch A, Stege A, and Lage H (2003) Modulation of the classical multidrug resistance (MDR) phenotype by RNA interference (RNAi). FEBS Lett 545:144-150.
31. Norris MD, Bordow SB, Marshall GM, Haber PS, Cohn SL, and Haber M (1996) Expression of the gene for multidrug-resistance-associated protein and outcome in patients with neuroblastoma. N Engl J Med 334:231-238.
32. Reid G, Wielinga P, Zelcer N, van der Heijden I, Kuil A, de Haas M, Wijnholds J, and Borst P (2003) The human multidrug resistance protein MRP4 functions as a prostaglandin efflux transporter and is inhibited by nonsteroidal antiinflammatory drugs. Proc Natl Acad Sci USA 100:9244-9249.
33. Schinkel AH and Jonker JW (2003) Mammalian drug efflux transporters of the ATP binding cassette (ABC) family: an overview. Adv Drug Deliv Rev 55:3-29.
34. Takikawa H, Yamazaki R, Sano N, and Yamanaka M (1996) Biliary excretion of estradiol-17 beta-glucuronide in the rat. Hepatology 23:607-613.
35. Wang H and LeCluyse EL (2003) Role of orphan nuclear receptors in the regulation of drug-metabolising enzymes. Clin Pharmacokinet 42:1331-1357.
36. Wu H, Hait WN, and Yang JM (2003) Small interfering RNA-induced suppression of MDR1 (P-glycoprotein) restores sensitivity to multidrug-resistant cancer cells. Cancer Res 63:1515-1519.
37. Young LC, Campling BG, Cole SP, Deeley RG, and Gerlach JH (2001) Multidrug resistance proteins MRP3, MRP1 and MRP2 in lung cancer: correlation of protein levels with drug response and messenger RNA levels. Clin Cancer Res 7:1798-1804.
38. Zamek-Gliszczynski MJ, Xiong H, Patel NJ, Turncliff RZ, Pollack GM, and Brouwer KL (2003) Pharmacokinetics of 5 (and 6)-carboxy-2′,7′- dichlorofluorescein and its diacetate promoiety in the liver. J Pharmacol Exp Ther 304:801-809.
39. Zhang P, Chandra P, Kramarcy N, and Brouwer KR (2001) Time course of multidrug resistance-associated protein (Mrp) 2 expression, localization and function in sandwich-cultured rat hepatocytes (SCRH) (Abstract). AAPS Pharm Sci 3:1522-1059.