Establishment of evaluation method for siRNA delivery using stable cell line carrying the luciferase reporter gene

Biological and Pharmaceutical Bulletin

Volumn 30, Issue 10, 2007, Pages 1844-1850

  Iijima, Ayako ^a   Hachisu, Rei ^b   Kobayashi, Hideo ^a   Hashimoto, Kouichi ^a   Asano, Daigo ^a   Kikuchi, Hiroshi ^a  

^a DAIICHI SANKYO CO   (Japan)

^b Custom Biotechnology Service Group   (Japan)

Author keywords

Cotransfection; Luciferase; RNA interference; Short interfering RNA; Transfection reagent

Indexed keywords

BETA GALACTOSIDASE; LIPOFECTAMINE; LUCIFERASE; PLASMID DNA; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR NFAT;

ARTICLE; CELL COUNT; CELL LINE; CHO CELL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; GENE DELIVERY SYSTEM; GENE EXPRESSION; GENETIC CODE; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; INCUBATION TIME; MISMATCH REPAIR; PLASMID; REPORTER GENE; RNA INTERFERENCE;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; DRUG CARRIERS; DRUG DELIVERY SYSTEMS; GENES, REPORTER; HELA CELLS; HUMANS; LIPOSOMES; LUCIFERASES; MICROSCOPY, CONFOCAL; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 35349027744     PISSN: 09186158     EISSN: 13475215     Source Type: Journal    
DOI: 10.1248/bpb.30.1844     Document Type: Article

References (8)

1. Fire A., Xu S., Montgomery M. K., Kostas S. A., Driver S. E., Mello C. C., Nature (London), 391, 806-811 (1998).
2. Kasim V., Taira K., Miyagishi M., J. Gene Med., 8, 782-791 (2006).
3. Ohki E. C., Tilkins M. L., Ciccarone V. C., Price P. J., J. Neurosci. Methods, 112, 95-99 (2001).
4. Rha S. J., Wang Y. P., J. Korean Med. Sci., 16, 567-572 (2001).
5. Kaul G., Amiji M., Pharm. Res., 22, 951-961 (2005).
6. Kikuchi A., Aoki Y., Sugiyama S., Serikawa T., Takakuwa K., Tanaka K., Suziki N., Kikuchi H., Hum. Gene Ther., 10, 947-955 (1999).
7. Akita H., Ito R., Khalil I. A., Futaki S., Harashima H., Mol. Ther., 9, 443-451 (2004).
8. Tinsley R. B., Vesey M. J., Barati S., Rush R. A., Ferguson I. A., J. Gene Med., 6, 1023-1032 (2004).