Substitution of synthetic chimpanzee androgen receptor for human androgen receptor in competitive binding and transcriptional activation assays for EDC screening

Toxicology Letters

Volumn 174, Issue 1-3, 2007, Pages 89-97

  Hartig, P C ^a   Cardon, M C ^a   Lambright, C R ^a   Bobseine, K L ^a   Gray Jr , L E ^a   Wilson, V S ^a  

^a U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

Author keywords

Adenovirus; Androgen; Baculovirus; Chimpanzee; Human; Receptor

Indexed keywords

ANDROGEN RECEPTOR; BACULOVIRUS VECTOR; ENDOCRINE DISRUPTOR; ESTROGEN RECEPTOR; HORMONE RECEPTOR; RECOMBINANT RECEPTOR;

ADENOVIRUS; ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; BINDING ASSAY; CHIMPANZEE; CHROMOSOME SUBSTITUTION; CHROMOSOME TRANSLOCATION; CONTROLLED STUDY; EUKARYOTE; GENE EXPRESSION; GENE SYNTHESIS; HUMAN; LIGAND BINDING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; RECEPTOR BINDING; SCREENING TEST; TRANSCRIPTION INITIATION; VIRUS REPLICATION;

ADENOVIRIDAE; ANDROGENS; ANIMALS; BACULOVIRIDAE; BINDING, COMPETITIVE; BIOLOGICAL ASSAY; CELL LINE; CELL NUCLEUS; CERCOPITHECUS AETHIOPS; COS CELLS; ENDOCRINE DISRUPTORS; GENETIC VECTORS; HUMANS; METRIBOLONE; PAN TROGLODYTES; PLASMIDS; RECEPTORS, ANDROGEN; RECOMBINANT PROTEINS; TRANS-ACTIVATION (GENETICS); TRANSDUCTION, GENETIC;

ADENOVIRIDAE; EUKARYOTA; MAMMALIA; PAN; VERTEBRATA;

EID: 35349018585     PISSN: 03784274     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxlet.2007.08.013     Document Type: Article

References (27)

1. Chang C., Wang C., DeLuca H.F., Ross T.K., and Shih C.C. Characterization of human androgen receptor overexpressed in the baculovirus system. Proc. Natl. Acad. Sci. U.S.A. 89 (1992) 5946-5950
2. Chang C.S., Kokontis J., and Liao S.T. Structural analysis of complementary DNA and amino acid sequences of human and rat androgen receptors. Proc. Natl. Acad. Sci. U.S.A. 85 (1988) 7211-7215
3. Choong C.S., Kemppainen J.A., and Wilson E.M. Evolution of the primate androgen receptor: a structural basis for disease. J. Mol. Evol. 47 (1998) 334-342
4. Colborn T. Pesticides-how research has succeeded and failed to translate science into policy: endocrinological effects on wildlife. Environ. Health Perspect. 103 Suppl. 6 (1995) 81-85
5. Freyberger A., and Ahr H.J. Development and standardization of a simple binding assay for the detection of compounds with affinity for the androgen receptor. Toxicology 195 (2004) 113-126
6. Graham F.L. Adenovirus vectors for high-efficiency gene transfer into mammalian cells. Immunol. Today 21 (2000) 426-428
7. Graham F.L., and Prevec L. Manipulation of adenovirus vectors. In: Murray E.J. (Ed). Methods in Molecular Biology vol. 7 (1991), The Humana Press Inc., Clifton, NJ 109-128
8. Guillette Jr. L.J., and Gunderson M.P. Alterations in development of reproductive and endocrine systems of wildlife populations exposed to endocrine-disrupting contaminants. Reproduction 122 (2001) 857-864
9. Hartig P.C., Bobseine K.L., Britt B.H., Cardon M.C., Lambright C.R., Wilson V.S., and Gray Jr. L.E. Development of two androgen receptor assays using adenoviral transduction of MMTV-luc reporter and/or hAR for endocrine screening. Toxicol. Sci. 66 (2002) 82-90
10. Hartig P.C., and Cardon M.C. Rapid efficient production of baculovirus expression vectors. J. Virol. Methods 38 (1992) 61-70
11. Hitt M., Bett A.J., Prevec L., and Graham F.L. Construction and propagation of human adenovirus vectors. In: Celis J.E. (Ed). Cell Biology: A Laboratory Handbook vol. 1 (1994), Academci Press, San Diego, CA 479-490
12. Janne O.A., Palvimo J.J., Kallio P., Mehto M., Xie Y.B., and Sui Y.P. Production of recombinant androgen receptor in a heterologous expression system. Clin. Chem. 39 (1993) 346-352
13. Lambright C., Ostby J., Bobseine K., Wilson V., Hotchkiss A.K., Mann P.C., and Gray Jr. L.E. Cellular and molecular mechanisms of action of linuron: an antiandrogenic herbicide that produces reproductive malformations in male rats. Toxicol. Sci. 56 (2000) 389-399
14. Lubahn D.B., Joseph D.R., Sar M., Tan J., Higgs H.N., Larson R.E., French F.S., and Wilson E.M. The human androgen receptor: complementary deoxyribonucleic acid cloning, sequence analysis and gene expression in prostate. Mol. Endocrinol. 2 (1988) 1265-1275
15. O'Reilly D.R., Miller L.K., and Luckow V.A. Baculovirus Expression Vectors: A Laboratory Manual (1992), W. H. Freeman and Company, New York, NY p. 347
16. Satta Y. Comparison of DNA and protein polymorphisms between humans and chimpanzees. Genes Genet. Syst. 76 (2001) 159-168
17. Shih W., Mears T., Bradley D.J., Parandoosh Z., and Weinberger C. An adenoviral vector system for functional identification of nuclear receptor ligands. Mol. Endocrinol. 5 (1991) 300-309
18. Simental J.A., Sar M., Lane M.V., French F.S., and Wilson E.M. Transcriptional activation and nuclear targeting signals of the human androgen receptor. J. Biol. Chem. 266 (1991) 510-518
19. Smith G.E., Summers M.D., and Fraser M.J. Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol. Cell. Biol. 3 (1983) 2156-2165
20. Summers M.D., and Smith G.E. A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures. Texas Agriculture Experiment Station Bulletin No. 1555 (1987), Texas A&M University, College Station, TX pp. 1-55
21. Tillmann M., Schulte-Oehlmann U., Duft M., Markert B., and Oehlmann J. Effects of endocrine disruptors on prosobranch snails (Mollusca: Gastropoda) in the laboratory. Part III: Cyproterone acetate and vinclozolin as antiandrogens. Ecotoxicology 10 (2001) 373-388
22. Wilson V.S., Bobseine K., Lambright C.R., and Gray Jr. L.E. A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists. Toxicol. Sci. 66 (2002) 69-81
23. Wilson V.S., Cardon M.C., Gray Jr. L.E., and Hartig P.C. Competitive binding comparison of endocrine-disrupting compounds to recombinant androgen receptor from fathead minnow, rainbow trout and human. Environ. Toxicol. Chem. 26 (2007) 1793-1802
24. Wilson V.S., Lambright C., Ostby J., and Gray Jr. L.E. In vitro and in vivo effects of 17beta-trenbolone: a feedlot effluent contaminant. Toxicol. Sci. 70 (2002) 202-211
25. Wong C.I., Zhou Z.X., Sar M., and Wilson E.M. Steroid requirement for androgen receptor dimerization and DNA binding. Modulation by intramolecular interactions between the NH2-terminal and steroid-binding domains. J. Biol. Chem. 268 (1993) 19004-19012
26. Xie Y.B., Sui Y.P., Shan L.X., Palvimo J.J., Phillips D.M., and Janne O.A. Expression of androgen receptor in insect cells. Purification of the receptor and renaturation of its steroid- and DNA-binding functions. J. Biol. Chem. 267 (1992) 4939-4948
27. Zhu Z., Bulgakov O.V., Scott S.S., and Dalton J.T. Recombinant expression and purification of human androgen receptor in a baculovirus system. Biochem. Biophys. Res. Commun. 284 (2001) 828-835