Development of the first Gateway firefly luciferase vector and use of reverse transcriptase in FLOE (Fluorescently Labeled Oligonucleotide Extension) reactions

Plasmid

Volumn 58, Issue 3, 2007, Pages 269-274

  Del Vecchio, Igor ^a   Zuccotti, Annalisa ^a   Canneva, Fabio ^a   Lenzken, Silvia Carolina ^a   Racchi, Marco ^a  

^a UNIVERSITY OF PAVIA   (Italy)

Author keywords

Firefly; FLOE; Gateway; GNB2L1; Luciferase vector; Primer extension; Promoter

Indexed keywords

DNA POLYMERASE; LUCIFERASE; OLIGONUCLEOTIDE; RNA DIRECTED DNA POLYMERASE;

ACCURACY; ARTICLE; CAPILLARY ELECTROPHORESIS; CELL LINE; CLONING VECTOR; DELETION MUTANT; FLUORESCENCE ANALYSIS; GATEWAY FIREFLY LUCIFERASE VECTOR; GENE CONTROL; GENE MAPPING; GENETIC MARKER; MONOCYTE; NERVE CELL CULTURE; NUCLEOTIDE SEQUENCE; REPORTER GENE; REVERSE TRANSCRIPTION; TRANSCRIPTION INITIATION SITE;

ANIMALS; CELL LINE; FIREFLIES; GENE EXPRESSION REGULATION; GENETIC VECTORS; LUCIFERASES, FIREFLY; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES; PROMOTER REGIONS (GENETICS); RNA-DIRECTED DNA POLYMERASE; TRANSFECTION;

EID: 35348992246     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2007.07.002     Document Type: Article

References (7)

1. de Wet J.R., Wood K.V., Helinski D.R., and DeLuca M. Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. Proc. Natl. Acad. Sci. USA 82 (1985) 7870-7873
2. Fekete R.A., Miller M.J., and Chattoraj D.K. Fluorescently labeled oligonucleotide extension: a rapid and quantitative protocol for primer extension. BioTechniques 35 (2003) 90-98
3. Landy A. Dynamic, structural, and regulatory aspects of lambda site-specific recombination. Annu. Rev. Biochem. 58 (1989) 913-949
4. Lorenz W.W., McCann R.O., Longiaru M., and Cormier M.J. Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proc. Natl. Acad. Sci. USA 88 (1991) 4438-4442
5. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual. second ed. (1989), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
6. Sanger F., Nicklen S., and Coulson A.R. DNA sequencing with chain-terminating inhibitors. 1977. Biotechnology 24 (1992) 104-108
7. Zhao H., Doyle T.C., Wong R.J., Cao Y., Stevenson D.K., Piwnica-Worms D., and Contag C.H. Characterization of coelenterazine analogs for measurements of Renilla luciferase activity in live cells and living animals. Mol. Imaging 3 (2004) 43-54