A novel culture technique for human embryonic stem cells using porous membranes

Stem Cells

Volumn 25, Issue 10, 2007, Pages 2601-2609

  Kim, Sinae ^a   Seong, Eun Ahn ^a   Jae, Ho Lee ^a   Lim, Do Seon ^b   Kim, Kwang Soo ^a,c   Chung, Hyung Min ^a   Lee, Soo Hong ^a  

^a CHA University   (South Korea)

^b Eulji University   (South Korea)

^c MCLEAN HOSPITAL   (United States)

Author keywords

Cell culture technique; Effective isolation of cultured cells; Human embryonic stem cells; No enzyme treatment; Porous membrane

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL CULTURE; CELL MEMBRANE; CELL MIGRATION; CELL STRUCTURE; CONTROLLED STUDY; EMBRYONIC STEM CELL; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; KARYOTYPE; MOUSE; NONHUMAN; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TERATOMA;

ANIMALS; ANTIGENS, DIFFERENTIATION; BIOLOGICAL MARKERS; CELL ADHESION; CELL CULTURE TECHNIQUES; CELL LINE; COCULTURE TECHNIQUES; DIFFUSION; EMBRYONIC STEM CELLS; FIBROBLASTS; GENE EXPRESSION PROFILING; HUMANS; KARYOTYPING; MEMBRANES, ARTIFICIAL; MICE; MICE, INBRED NOD; MICE, SCID; TERATOMA;

EID: 35348880251     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1634/stemcells.2006-0814     Document Type: Article

References (20)

1. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al. Embryonic stem cell lines derived from human blastocysts. Science 1998;282:1145-1147.
2. Reubinoff BE, Pera MF, Fong CY et al. Embryonic stem cell lines from human blastocysts: Somatic differentiation in vitro. Nat Biotechnol 2000; 18:399-404.
3. Xu C, Inokuma MS, Denham J et al. Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol 2001;19:971-974.
4. Richards M, Fong CY, Chan WK et al. Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol 2002;20:933-936.
5. Hovatta O, Mikkola M, Gertow K et al. A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum Reprod 2003;18:1404-1409.
6. Buzzard JJ, Gough NM, Crook JM et al. Karyotype of human ES cells during extended culture. Nat Biotechnol 2004;22:381-382.
7. Mitalipova MM, Rao RR, Hoyer DM et al. Preserving the genetic integrity of human embryonic stem cells. Nat Biotechnol 2005;23:19-20.
8. Heins N, Englund MC, Sjoblom C et al. Derivation, characterization, and differentiation of human embryonic stem cells. STEM CELLS 2004;22:367-376.
9. Oh SK, Kim HS, Park YB et al. Methods for expansion of human embryonic stem cells. STEM CELLS 2005;23:605-609.
10. Joannides A, Fiore-Heriche C, Westmore K et al. Automated mechanical passaging: a novel and efficient method for human embryonic stem cell expansion. STEM CELLS 2006;24:230-235.
11. Stojkovic M, Lako M, Strachan T et al. Derivation, growth and applications of human embryonic stem cells. Reproduction 2004;128:259-267.
12. Amit M, Itskovitz-Eldor J. Maintenance of human embryonic stem cells in animal serum- and feeder layer-free culture conditions. Methods Mol Biol 2006;331:105-113.
13. Cheng L, Hammond H, Ye Z et al. Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture. STEM CELLS 2003;21:131-142.
14. Richards M, Tan S, Fong CY et al. Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells. STEM CELLS 2003;21:546-556.
15. Amit M, Margulets V, Segev H et al. Human feeder layers for human embryonic stem cells. Biol Reprod 2003;68:2150-2156.
16. Klimanskaya I, Chung Y, Meisner L et al. Human embryonic stem cells derived without feeder cells. Lancet 2005;365:1636-1641.
17. Xu C, Rosler E, Jiang J et al. Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium. STEM CELLS 2005;23:315-323.
18. Ahn SE, Kim S, Park KH et al. Primary bone-derived cells induce osteogenic differentiation without exogenous factors in human embryonic stem cells. Biochem Biophys Res Commun 2006;340:403-408.
19. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001;25:402- 408.
20. Dutrillaux B, Viegas-Pequignot E. High resolution R- and G-banding on the same preparation. Hum Genet 1981;57:93-95.