Plasmon-waveguide resonance (PWR) spectroscopy for directly viewing rates of GPCR/G-protein interactions and quantifying affinities

Current Opinion in Pharmacology

Volumn 7, Issue 5, 2007, Pages 507-514

  Hruby, Victor J ^a,b   Tollin, Gordon ^a,b  

^a University of Arizona   (United States)

^b UNIVERSITY OF ARIZONA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPRENOLOL; BETA 2 ADRENERGIC RECEPTOR; CANNABINOID 1 RECEPTOR; DELTA OPIATE RECEPTOR; G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; ISOPRENALINE; NALTRINDOLE; SILICON DIOXIDE;

BINDING AFFINITY; DRUG TARGETING; HUMAN; LIGAND BINDING; LIPID BILAYER; PLASMON WAVEGUIDE RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN IMMOBILIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; QUANTITATIVE ANALYSIS; REVIEW; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; SPECTROSCOPY; SURFACE PLASMON RESONANCE;

GTP-BINDING PROTEINS; HUMANS; LIGANDS; PROTEIN BINDING; RECEPTORS, G-PROTEIN-COUPLED; SPECTRUM ANALYSIS; SURFACE PLASMON RESONANCE;

EID: 35248835516     PISSN: 14714892     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.coph.2007.08.001     Document Type: Review

References (18)

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14. Alves I.D., Cowell S.M., Salamon Z., Devanathan S., Tollin G., and Hruby V.J. Different structural states of the proteolipid membrane are produced by ligand binding to the human δ-opioid receptor as shown by plasmon-waveguide resonance spectroscopy. Mol Pharmacol 65 (2004) 1248-1257. The first direct demonstration of the exceptionally high degree of structural plasticity of a GPCR.
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16. Georgieva T, Devanathan S, Stropova D, Park CK, Salamon Z, Tollin G, Hruby VJ, Roeske WR, Yamamura HI, Varga E: Unique agonist-bound CB1 receptor conformations indicate agonist specificity in signaling. Manuscript submitted for publication.
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