Bacteria metabolically engineered for enhanced phytochelatin production and cadmium accumulation

Applied and Environmental Microbiology

Volumn 73, Issue 19, 2007, Pages 6317-6320

  Seung, Hyun Kang ^a   Singh, Shailendra ^a,b   Kim, Jae Young ^a,c   Lee, Wonkyu ^a,d   Mulchandani, Ashok ^a   Chen, Wilfred ^a  

^a University of California Riverside   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c Cornell University   (United States)

^d Ajou University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

FEEDBACK INHIBITION; MICROBIAL SORBENTS; PHYTOCHELATINS (PC); SCHIZOSACCHAROMYCES POMBE (SPPCS);

CADMIUM COMPOUNDS; ESCHERICHIA COLI; GAMMA RAYS; HEAVY METALS; POLYPEPTIDES;

BACTERIA;

CADMIUM; CARRIER PROTEIN; ENZYME VARIANT; GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE; PHYTOCHELATIN; PHYTOCHELATIN SYNTHASE; PROTEIN MNTA; UNCLASSIFIED DRUG;

BIOACCUMULATION; CADMIUM; COLIFORM BACTERIUM; PEPTIDE;

ARTICLE; BACTERIAL STRAIN; BINDING AFFINITY; BIOACCUMULATION; CONTROLLED STUDY; ESCHERICHIA COLI; GENETIC ENGINEERING; HEAVY METAL REMOVAL; NEGATIVE FEEDBACK; NONHUMAN; PROTEIN EXPRESSION; SCHIZOSACCHAROMYCES POMBE; SYNTHESIS;

BACTERIA; CADMIUM; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; PHYTOCHELATINS;

ESCHERICHIA COLI; SCHIZOSACCHAROMYCES POMBE;

EID: 35148883279     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01237-07     Document Type: Article

References (21)

1. Bae, W., R. Mehra, A. Mulchandani, and W. Chen. 2001. Genetic engineering of Escherichia coli for enhanced bioaccumulation of mercury. Appl. Environ. Microbiol. 67:5335-5338.
2. Bae, W., W. Chen, A. Mulchandani, and R. Mehra. 2000. Enhanced bioaccumulation of heavy metal by bacterial cells displaying synthetic phytochelatins. Biotechnol. Bioeng. 70:518-524.
3. Clemens, S., E. J. Kim, D. Neumann, and J. I. Schroeder. 1999. Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast. EMBO J. 18:3325-3333.
4. Hao, Z., S. Chen, and D. B. Wilson. 1999. Cloning, expression, and characterization of cadmium and manganese uptake genes from Lactobacillus plantarum. Appl. Environ. Microbiol. 65:4746-4752.
5. Kelly, B. S., W. E. Antholine, and W. Griffith. 2002. Escherichia coli γ-glutamylcysteine synthetase. Two active site metal ions affect substrate and inhibitor binding. J. Biol. Chem. 277:50-58.
6. Kim, S. K., B. S. Lee, D. B. Wilson, and E. K. Kim. 2005. Selective cadmium accumulation using recombinant Escherichia coli. J. Biosci. Bioeng. 99:109-114.
7. Maitani, T., H. Kubota, K. Sato, and T. Yamada. 1996. The composition of metals bound to class III metallothionein (phytochelatin and its desglycyl peptide) induced by various metals in root cultures of Rubia tinctorum. Plant Physiol. 110:1145-1150.
8. Malik, A. 2004. Metal bioremediation through growing cells. Environ. Int. 30:261-278.
9. Meister, A. 1995. Glutathione biosynthesis and its inhibition. Methods Enzymol. 252:26-30.
10. Mejare, M., and L. Bulow. 2001. Metal-binding proteins and peptides in bioremediation and phytoremediation of heavy metals. Trends Biotechnol. 19:67-73.
11. Murata, K., and A. Kimura. 1982. Cloning of a gene responsible for the biosynthesis of glutathione in Escherichia coli B. Appl. Environ. Microbiol. 44:1444-1448.
12. Nriagu, J. O., and J. M. Pacyna. 1988. Quantitative assessment of worldwide contamination of air, water and soils by trace metals. Nature 333:134-139.
13. Rui, L., Y. M. Kwon, K. F. Reardon, and T. K. Wood. 2004. Metabolic pathway engineering to enhance aerobic degradation of chlorinated ethenes and to reduce their toxicity by cloning a novel glutathione S-transferase, an evolved toluene o-monooxygenase, and γ-glutamylcysteine synthetase. Environ. Microbiol. 6:491-500.
14. Sambrook, J., E. F. Fritsch, and T. Maniatis. 2001. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. Sauge-Merle, S., S. Cuine, P. Carrier, C. Lecomte-Pradines, D. T. Luu, and G. Peltier. 2003. Enhanced toxic metal accumulation in engineered bacterial cells expressing Arabidopsis thaliana phytochelatin synthase. Appl. Environ. Microbiol. 69:490-494.
16. Schmoger, M. E. V., M. Oven, and E. Grill. 2000. Detoxification of arsenic by phytochelatins in plants. Plant Physiol. 122:793-801.
17. Sneller, F. E. C., L. M. Heerwaarden, P. L. M. Koevoets, R. Vooijs, H. Schat, and J. A. C. Verkleij. 2000. Derivatization of phytochelatins from Silence vulgaris, induced upon exposure to arsenate and cadmium: comparison of derivatization with Ellman's reagent and monobromobimane. J. Agric. Food. Chem. 48:4014-4019.
18. Sousa, C., P. Kotrba, T. Ruml, A. Cebolla, and V. D. Lorenzo. 1998. Metalloadsorption by Escherichia coli cells displaying yeast and mammalian metallothioneins anchored to the outer membrane protein LamB. J. Bacteriol. 180:2280-2284.
19. + by a Mesorhizobium sp. transformed with a gene from Arabidopsis thaliana coding for phytochelatin synthase. Appl. Environ. Microbiol. 69:1791-1796.
20. Wawrzynska, A., D. Gaganidze, E. Kopera, K. Piatek, W. Bal, and A. Sirko. 2005. Overexpression of genes involved in phytochelatin biosynthesis in Escherichia coli: effects on growth, cadmium accumulation and thiol level. Acta Biochim. Pol. 52:109-116.
21. Zenk, M. H. 1996. Heavy metal detoxification in higher plants: a review. Gene 179:21-30.