Measurements with fluorescent probes in primary neural cultures; improved multiwell techniques

Journal of Pharmacological and Toxicological Methods

Volumn 56, Issue 3, 2007, Pages 300-307

  Ring, Avi ^a   Tanso, Rita ^a  

^a NORWEGIAN DEFENCE RESEARCH ESTABLISHMENT FFI   (Norway)

Author keywords

Calcium; Cerebellar granule cells; Drug discovery; Fluorescence; Fura 2; Ion channel block; Methods; MK 801; NMDA; Rat

Indexed keywords

CALCIUM; DIZOCILPINE; FURA 2; GLYCINE; ION CHANNEL; MAGNESIUM; N METHYL DEXTRO ASPARTIC ACID; N METHYL DEXTRO ASPARTIC ACID RECEPTOR BLOCKING AGENT;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CALCIUM CELL LEVEL; CELL ADHESION; CELL DIFFERENTIATION; CELL STRUCTURE; CONTROLLED STUDY; DENDRITIC CELL; EXCITOTOXICITY; FLUORESCENCE; IC 50; NERVE CELL CULTURE; NONHUMAN; RADIOMETRY; RAT; SYNAPSE; ULTRAVIOLET RADIATION;

ANIMALS; CALCIUM; CALCIUM SIGNALING; CARBACHOL; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CEREBELLUM; DIZOCILPINE MALEATE; DOSE-RESPONSE RELATIONSHIP, DRUG; EXCITATORY AMINO ACID AGONISTS; EXCITATORY AMINO ACID ANTAGONISTS; FLUORESCENCE; FLUORESCENT DYES; FURA-2; GLYCINE; INHIBITORY CONCENTRATION 50; N-METHYLASPARTATE; NEURONS; PROCYCLIDINE; RATS; RECEPTORS, N-METHYL-D-ASPARTATE; REPRODUCIBILITY OF RESULTS;

RATTUS;

EID: 35148817465     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vascn.2007.05.004     Document Type: Article

References (24)

1. Ascher P., and Nowak L. The role of divalent cations in the N-methyl-d-aspartate responses of mouse central neurones in culture. Journal of Physiology 399 (1988) 247-266
2. 2+ mobilization through inositol(1,4,5)trisphosphate or ryanodine receptors in cerebellar granule cells in primary culture. European Journal of Neuroscience 11 (1999) 3015-3022
3. 2+ uptake in cultured cerebral cortex neurons. In: Wray D.W., Norman R.I., and Hess P. (Eds). Calcium channels: structure and function. Annals of the New York Academy of Sciences Vol. 560 (1989) 454-455
4. Gallo V., Ciotti M.T., Coletti A., Aloisi F., and Levi G. Selective release of glutamate from cerebellar granule cells differentiating in culture. Proceedings of the National Academy of Sciences of the United States of America 79 (1982) 7919-7923
5. Goslin K., Asmussen A., and Banker A. Rat hippocampal neurons in low-density culture. In: Banker G., and Goslin K. (Eds). Culturing nerve cells. 2nd ed. (1998), The MIT Press, Cambridge Massachusets 344-346
6. 2+ indicators with greatly improved fluorescence properties. Journal of Biological Chemistry 260 (1985) 3440-3450
7. Jahr C.E., and Stevens C.F. A quantitative description of NMDA receptor-channel kinetic behavior. Journal of Neuroscience 10 (1990) 1830-1837
8. 2+] with fluorescent indicators. Methods in Cell Biology 40 (1994) 155-181
9. Kleckner N.W., and Dingledine R. Regulation of hippocampal NMDA receptors by magnesium and glycine during development. Brain Research, Molecular Brain Research 11 (1991) 151-159
10. Kleinfeld D., Kahler K.H., and Hockberger P.E. Controlled outgrowth of dissociated neurons on patterned substrates. Journal of Neuroscience 8 (1988) 4098-4120
11. Kupper J., Ascher P., and Neyton J. Probing the pore region of recombinant N-methyl-d-aspartate channels using external and internal magnesium block. Proceedings of the National Academy of Sciences of the United States of America 93 (1996) 8648-8653
12. 2+-block site in NR1-1a/NR2A NMDA receptor channels expressed in Xenopus oocytes. Life Science 68 (2001) 1817-1826
13. Mason W.T. Fluorescent and luminescent probes for biological activity; A practical guide to technology for quantitative real-time analysis. 2nd ed. (1999), Academic Press, London, UK
14. McDonough Jr. J.H., and Shih T.M. A study of the N-methyl-d-aspartate antagonistic properties of anticholinergic drugs. Pharmacology, Biochemistry and Behavior 51 (1995) 249-253
15. Miyazaki J., Nakanishi S., and Jingami H. Expression and characterization of a glycine-binding fragment of the N-methyl-d-aspartate receptor subunit NR1. Biochemical Journal 340 (1999) 687-692
16. Motulsky H., and Christopoulos A. How does a treatment change a curve?. Fitting models to biological data using linear and nonlinear regression. A practical guide to curve fitting (2004), Oxford University Press, New York, USA 160-162
17. Neher E. Combined fura-2 and patch clamp measurements in rat peritonial mast cells. In: Sellin L.C., Libelius R., and Thesleff S. (Eds). Neuromuscular junction (1989), Elsevier, Amsterdam, Holland 65-76
18. Parks T.N., Artman L.D., Alasti N., and Nemeth E.F. Modulation of N-methyl-d-aspartate receptor-mediated increases in cytosolic calcium in cultured rat cerebellar granule cells. Brain Research 552 (1991) 13-22
19. Schousboe A., Meier E., Drejer J., and Hertz L. Preparation of cultures of mouse (rat) cerebellar granule cells. In: Shaha A., de Vellis J., Vernadakis A., and Haber B. (Eds). A dissection and tissue culture manual for the nervous system (1989), Alan R. Liss, New York, USA 203-206
20. Smith A. Screening for drug discovery: the leading question. Nature 418 (2002) 453-459
21. Tsien R.Y., and Harootunian A.T. Practical design criteria for a dynamic ratio imaging system. Cell Calcium 11 (1990) 93-109
22. 2+. Molecular Pharmacology 53 (1998) 933-941
23. Young K.W., Garro M.A., Challiss R.A., and Nahorski S.R. NMDA-receptor regulation of muscarinic-receptor stimulated inositol 1,4,5-trisphosphate production and protein kinase C activation in single cerebellar granule neurons. Journal of Neurochemistry 89 (2004) 1537-1546
24. Yuste R., Lanni F., and Konnerth A. Imaging neurons: a laboratory manual (2000), Cold Spring Harbor Laboratory Press, New York, USA