Quantitative (real-time) PCR

Methods in Gut Microbial Ecology for Ruminants

Volumn , Issue , 2005, Pages 105-115

  Denman, Stuart E ^a   McSweeney, Christopher S ^a  

^a CSIRO   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 34948858813     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/1-4020-3791-0_8     Document Type: Chapter

References (15)

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3. Freeman, W. M., S. J. Walker, and K. E. Vrana. 1999. Quantitative RT-PCR: pitfalls and potential. Biotechniques 26:112-125.
4. Klieve, A. V., D. Hennessy, D. Ouwerkerk, R. J. Forster, R. I. Mackie, and G. T. Attwood. 2003. Establishing populations of Megasphaera elsdenii YE 34 and Butyrivibrio fibrisolvens YE 44 in the rumen of cattle fed high grain diets. J. Appl. Microbiol. 95:621-630.
5. Koike, S., and Y. Kobayashi. 2001. Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FEMS Microbiol. Lett. 204:361-366.
6. Krause, D., B. Dalrymple, W. Smith, R. Mackie, and C. McSweeney. 1999. 16S rDNA sequencing of Ruminococcus albus and Ruminococcus flavefaciens: design of a signature probe and its application in adult sheep. Microbiology 145:1797-1807.
7. Lane, D. J. 1991. 16S/23S rRNA sequencing, pp. 115-175. In E. Stackebrandt and M. D. Goodfellow (eds.), Nucleic Acid Techniques in Bacterial Systematics. John Wiley and Sons, New York, NY.
8. Lin, C., and D. A. Stahl. 1995. Taxon-specific probes for the cellulolytic genus fibrobacter reveal abundant and novel equine-associated populations. Appl. Environ. Microbiol. 61:1348-1351.
9. McSweeney, C. S., R. I. Mackie, A. A. Odenyo, and D. A. Stahl. 1993. Development of an oligonucleotide probe targeting 16S rRNA and its application for detection and quantitation of the ruminal bacterium Synergistes jonesii in a mixed-population chemostat. Appl. Environ. Microbiol. 59:1607-1612.
10. Odenyo, A. A., R. I. Mackie, D. A. Stahl, and B. A. White. 1994. The use of 16S rRNA-targeted oligonucleotide probes to study competition between ruminal fibrolytic bacteria: pure-culture studies with cellulose and alkaline peroxide-treated wheat straw. Appl. Environ. Microbiol. 60:3697-3703.
11. Ouwerkerk, D., A. V. Klieve, and R. J. Forster. 2002. Enumeration of Megasphaera elsdenii in rumen contents by real-time Taq nuclease assay. J. Appl. Microbiol. 92:753-758.
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13. Rasmussen, R. 2001. Quantification on the LightCycler, pp. 21-34. In S. Meuer, C. Wittwer, and K. Nakagawara (eds.), Rapid Cycle Real-Time PCR. Methods and Applications Springer Press, Heidelberg.
14. Reilly, K., and G. T. Attwood. 1998. Detection of Clostridium proteoclasticum and closely related strains in the rumen by competitive PCR. Appl. Environ. Microbiol. 64:907-913.
15. Tajima, K., R. I. Aminov, T. Nagamine, H. Matsui, M. Nakamura, and Y. Benno. 2001. Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Appl. Environ. Microbiol. 67:2766-2774.