Establishment and characterization of a primary canine duodenal epithelial cell culture

In Vitro Cellular and Developmental Biology - Animal

Volumn 43, Issue 5-6, 2007, Pages 176-185

  Golaz, Julia L ^a   Vonlaufen, Nathalie ^a   Hemphill, Andrew ^a   Burgener, Iwan A ^a  

^a UNIVERSITY OF BERN   (Switzerland)

Author keywords

Chronic enteropathies; Dog; Host pathogen interaction; Intestine; Neonatal

Indexed keywords

ALKALINE PHOSPHATASE; ANTIBODY; COLLAGENASE; CYTOKERATIN; CYTOKERATIN ANTIBODY; DISPASE; EDETIC ACID; EPIDERMAL GROWTH FACTOR; HYDROCORTISONE; INSULIN; KI 67 ANTIGEN; MONOCLONAL ANTIBODY KI 67; SORBITOL; SUCRASE; BIOLOGICAL MARKER;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CELL GROWTH; CELL SUBPOPULATION; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; DESMOSOME; DOG; DUODENUM; ELECTRON MICROSCOPY; ENZYMATIC DEGRADATION; ENZYME ACTIVITY; FETAL CALF SERUM; IMMUNOFLUORESCENCE TEST; INCUBATION TEMPERATURE; INTESTINE CRYPT; INTESTINE EPITHELIUM CELL; MONOLAYER CULTURE; NEWBORN; NONHUMAN; ANIMAL; CELL CULTURE; CELL LINE; CULTURE TECHNIQUE; CYTOLOGY; EPITHELIUM CELL; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOBLOTTING; INTERMEDIATE FILAMENT; METABOLISM; METHODOLOGY; ULTRASTRUCTURE;

CANIS FAMILIARIS;

ANIMALS; ANIMALS, NEWBORN; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; CELL LINE; CELLS, CULTURED; DOGS; DUODENUM; EPITHELIAL CELLS; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOBLOTTING; INTERMEDIATE FILAMENTS;

EID: 34848928983     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11626-007-9034-4     Document Type: Article

References (29)

1. Aldhous, M. C.; Shmakov, A. N.; Bode, J., et al. Characterization of conditions for the primary culture of human small intestinal epithelial cells. Clin. Exp. Immunol. 125:32-40.2001.
2. Allenspach, K.; Gaschen, F. Chronic intestinal diseases in the dog: a review. Schweiz. Arch. Tierheilkd. 145(5):209-219, 221-2.2003.
3. Calnek, D.; Quaroni, A. Differential localization by in situ hybridization of distinct keratin mRNA species during intestinal epithelial cell development and differentiation. Differentiation 53:95-104.1993.
4. Duchmann, R. I.; Kaiser, E.; Hermann, W., et al. Tolerance exists towards resident intestinal flora but is broken in active inflammatory bowel disease (IBD). Clin. Exp. Immunol. 102:448-455.1995.
5. Engelhardt, P.; Wyder, M.; Zurbriggen, A., et al. Canine disempter virus associated proliferation of canine footpad keratinocytes in vitro. Vet. Microbiol. 107:1-12.2005.
6. Evans, G. S.; Flint, N.; Somers, A. S., et al. The development of a method for the preparation of rat intestinal epithelial cell primary cultures. J. Cell Sci. 101:219-231.1992.
7. Föllmann, W.; Weber, S.; Birkner, S. Primary cell cultures of bovine colon epithelium: isolation and cell culture of colonocyte. Toxicol. In Vitro 14:435-445.2000.
8. Fre, S.; Huyghe, M.; Mourikis, P., et al. Notch signals control the fate of immature progenitor cells in the intestine. Nature 435:964-968.2005.
9. German, A. J.; Hall, E. J.; Day, M. J. Chronic intestinal inflammation and intestinal disease in dogs. J. Vet. Intern. Med. 17:8-20.2003.
10. Grossmann, J.; Walther, K.; Artinger, M., et al. Progress on isolation and short-term ex-vivo culture of highly purified non-apoptotic human intestinal cells (IEC). Eur. J. Cell. Biol. 82:262-270.2003.
11. Hauck, A. L.; Swanson, K. S.; Kenis, P. J. A., et al. Twists and turns in the development and maintenance of the mammalian small intestine epithelium. Birth Defects Res C Embryo Today 75:58-71.2005.
12. Hemphill, A.; Croft, S. L. Electron microscopy in parasitology. In: Rogan, M. ed. Analytical parasitology. Springer Verlag, Heidelberg, Germany; 1997:227-268.
13. Hershberg, R. M.; Mayer, L. F. Antigen processing and presentation by intestinal epithelial cells-polarity and complexity. Immunol. Today 21:123-128.2000.
14. Jung, H. C.; Eckmann, L.; Yang, S. K., et al. A distinct array of proinflammatory cytokines is expressed in human colon epithelial cells in response to bacterial invasion. J. Clin. Invest. 95:55-65.1995.
15. Kaeffer, B. Mammalian intestinal epithelial cells in primary culture: a mini-review. In Vitro Cell Dev. Biol. Anim. 38:123-134.2002.
16. Kedinger, M.; Duluc, I.; Fritsch, C., et al. Intestinal epithelial-mesenchymal cell interactions. Ann. N Y Acad. Sci. 859:1-17.1998.
17. Pageot, L. P.; Perreault, N.; Basora, N., et al. Human cell models to study small intestinal functions: recapitulation of the crypt-villus axis. Microsc. Res. Tech. 49:394-406.2000.
18. Panja, A. A novel method for the establishment of a pure population of nontransformed human intestinal primary epithelial cell (HIPEC) lines in long term culture. Lab. Invest. 80:1473-1475.2000.
19. Perreault, N.; Beaulieu, J. F. Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures. Exp. Cell Res. 224:354-364.1996.
20. Perreault, N.; Beaulieu, J. F. Primary cultures of fully differentiated and pure human intestinal epithelial cells. Exp. Cell. Res. 245:34-42.1998.
21. Plotkin, G. R.; Isselbacher, K. J. Secondary disaccharidase deficiency in adult celiac disease (nontropical sprue) and other malabsorption states. New Engl. J. Med. 271:1033-1037.1964.
22. Quaroni, A.; Hochman, J. Development of intestinal cell culture models for drug transport and metabolism studies. Adv. Drug Deliv. Res. 22:3-52.1996.
23. Quaroni, A.; Tian, J. Q.; Göke, M., et al. Glucocorticoids have pleiotropic effects on small intestinal crypt cells. Am. J. Physiol. Gastrointest. Liver Physiol. 277:G1027-G1040.1999.
24. Saiwaki, T.; Kotera, I.; Sasaki, M., et al. In vivo dynamics and kinetics of pKi-67: transition from a mobile to an immobile form at the onset of anaphase. Exp. Cell Res. 308:123-134.2005.
25. Savkovic, S. D.; Koutsouris, A.; Hecht, G. Activation of NF-kappaB in intestinal epithelial cells by enteropathogenic Escherichia coli. Am. J. Physiol. 273:C1160-C1167.1997.
26. Stettler, M.; Siles-Lucas, M.; Sarciron, E., et al. Echinococcus multilocularis alkaline phosphatase as a marker for metacestode damage induced by in vitro drug treatment with albendazole sulfoxide and albendazole sulfone. Antimicrob. Agents Chemother. 45:2256-2262.2001.
27. Swerdlow, M.; Kennedy, D.; Clayton, D., et al. Cultured colonic epithelial cells express pattern recognition receptors. In Proceedings 22nd ACVIM Forum 853.2004.
28. Weng, X. H.; Beyenbach, K. W.; Quaroni, A. Cultured monolayers of the dog jejunum with the structural and functional properties resembling the normal epithelium. Am. J. Physiol. Gastrointest. Liver Physiol. 288:G705-G717.2005.
29. Wong, M. H. Regulation of intestinal stem cells. J. Investig. Dermatol. Symp. Proc. 9:224-228.2004.