Purification and characterization of Enterobacter sakazakii enterotoxin

Canadian Journal of Microbiology

Volumn 53, Issue 6, 2007, Pages 750-755

  Raghav, Mamta ^a   Aggarwal, P K ^a  

^a NATIONAL DAIRY RESEARCH INSTITUTE   (India)

Author keywords

Enterobacter sakazakii; Enterotoxin; Gel filtration; Pathogen; Purification

Indexed keywords

AMMONIUM COMPOUNDS; CHARACTERIZATION; CHEMICAL STABILITY; MOLECULAR BIOLOGY; PATHOGENS; PRECIPITATION (CHEMICAL); PURIFICATION; TOXIC MATERIALS;

ENTEROBACTER SAKAZAKII; ENTEROTOXIN; GEL FILTRATION;

BACTERIA;

AMMONIUM SULFATE; DIETHYLAMINOETHYL CELLULOSE; ENTEROTOXIN; PRONASE; SEPHADEX; TRYPSIN;

BACTERIUM; MILK; NEONATE; PATHOGEN; TOXIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; ARTIFICIAL MILK; CONTROLLED STUDY; ENTEROBACTER INFECTION; ENTEROBACTER SAKAZAKII; FOOD CONTAMINATION; ION EXCHANGE; MOUSE; NEWBORN CARE; NONHUMAN; PH MEASUREMENT; PRECIPITATION; PRIORITY JOURNAL; PURIFICATION; RISK FACTOR;

AMMONIUM SULFATE; CHROMATOGRAPHY, DEAE-CELLULOSE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENTEROBACTER SAKAZAKII; ENTEROTOXINS; HYDROGEN-ION CONCENTRATION; PRONASE; TEMPERATURE; TRYPSIN;

ENTEROBACTER SAKAZAKII;

EID: 34848928508     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W07-037     Document Type: Article

References (20)

1. Aggarwal, P.K., and Srinivasan, R.A. 1987. Toxic activities of Bacillus cereus in milk and khoa. NDRI. Dia. Jubl. Comm. Vol., Part III: IDA Sci. Symp., 595-604. NDRI, Karnal, India.
2. Arseni, A., Malamou-Ladas, E., Koutsia, C., Xanthou, M., and Trikka, E. 1987. Outbreak of colonization of neonates with Enterobacter sakazakii. J. Hosp. Infect. 9: 143-150. doi:10.1016/0195-6701(87)90052-1.
3. Bar-Oz, B., Preminger, A., Peleg, O., Block, C., and Arad, I. 2001. Enterobacter sakazakii infection in the newborn. Acta Paediatr. 90: 356-358.
4. Biering, G., Karlsson, S., Clark, N.V.C., Jonsdottir, K.E., Ludvigsson, P., and Steingrimsson, O. 1989. Three cases of neonatal meningitis caused by Enterobacter sakazakii in powdered milk. J. Clin. Microbiol. 27: 2054-2056.
5. Challahan, L.T. III. 1974. Purification and characterization of Pseudomonas aeruginosa exotoxin. Infect. Immun. 9: 113-118.
6. Dean, A.G., Ching, Y.C., Williams, R.G., and Harden, L.B. 1972. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honululu. J. Infect. Dis. 125: 407-411.
7. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356. doi:10.1021/ac60111a017.
8. Dubreuil, J.D., Fairbrother, J.M., Lallier, R., and Lariviere, S. 1991. Production and purification of heat stable enterotoxin b from a porcine Escherichia coli strain. Infect. Immun. 59: 198-203.
9. Evans, D.J., Evans, D.G., and Gorbach, S.L. 1973. Production of vascular permeability factor by enterotoxigenic Eschericia coli isolated from man. Infect. Immun. 8: 725-730.
10. Farmer, J.J., Asbury, M.A., Hickman, F.W., and Brenner, D.J., and the Enterobacteriaceae Study Group. 1980. Enterobacter sakazakii, new species of Enterobacteriaceae isolated from clinical specimens. Int. J. Syst. Bacteriol. 30: 569-584.
11. Gurtler, J.B., Kornacki, J.L., and Beuchat, L.R. 2005. Enterobacter sakazakii: a coliform of increased concern to infant health. Int. J. Food Microbiol. 104: 1-34. doi:10.1016/j.ijfoodmicro.2005.02.013.
12. Jacks, T.M., and Wu, B.J. 1974. Biochemical properties of Escherichia coli low molecular weight, heat-stable enterotoxin. Infect. Immun. 9: 342-347.
13. Keusch, G.T., Grady, G.F., Mata, L.J., and McIver, J. 1972. The pathogenesis of Shigella diarrhea.I. Enterotoxin production by Shigella dysenteriae 1. J. Clin. Invest. 51: 1212-1218.
14. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London), 227: 680-685. doi:10.1038/227680a0.
15. Lai, K.K. 2001. Enterobacter sakazakii infections among neonates, infants, children, and adults: case reports and a review of the literature. Medicine, 80: 113-122. doi:10.1097/00005792-200103000-00004.
16. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275.
17. Pagotto, F.J., Nazarowec-White, M., Bidawid, S., and Farber, J.M. 2003. Enterobacter sakazakii: infectivity and enterotoxin production in vitro and in vivo. J. Food Prot. 66: 370-377.
18. Van Acker, J., De Smet, F., Muyldermans, G., Bougatef, A., Naessens, A., and Lauwers, S. 2001. Outbreak of necrotizing enterocolitis associated with Enterobacter sakazakii in powdered milk formula. J. Clin. Microbiol. 39: 293-297. doi:10.1128/JCM.39.1.293-297.2001.
19. Whipp, S.C., Moon, H.W., and Lyon, N.C. 1975. Heat stable Escherichia coli enterotoxin produced in mice. Infect. Immun. 12: 240-244.
20. Yutsudo, T., Honda, T., Miwatani, T., and Takeda, Y. 1986. Characterization of purified Shiga toxin from Shigella dysenteriae 1. Microbiol. Immunol. 30: 1115-1127.