Cell suspension cultures of allogenic keratinocytes are efficient carriers for ex vivo gene transfer and accelerate the healing of full-thickness skin wounds by overexpression of human epidermal growth factor

Wound Repair and Regeneration

Volumn 15, Issue 5, 2007, Pages 657-664

  Vranckx, Jan Jeroen ^a,b   Hoeller, Daniela ^a,c   Velander, Patrik E M ^a   Theopold, Christoph F P ^a   Petrie, Nicola ^a   Takedo, Akira ^a   Eriksson, Elof ^a   Yao, Feng ^a  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

^b UNIVERSITY HOSPITALS LEUVEN   (Belgium)

^c UNIVERSITY HOSPITAL RWTH AACHEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GAMMA UROGASTRONE; SODIUM CHLORIDE;

ALLOGRAFT; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL BASED GENE THERAPY; CELL CULTURE; CELL SUSPENSION; CONTROLLED STUDY; EX VIVO STUDY; FEMALE; GENE DELIVERY SYSTEM; GENE OVEREXPRESSION; GENE TRANSFER; KERATINOCYTE; NONHUMAN; PLASMID; PRIORITY JOURNAL; SKIN INJURY; STATISTICAL SIGNIFICANCE; SWINE; WOUND HEALING;

ANIMALS; CELLS, CULTURED; EPIDERMAL GROWTH FACTOR; EPITHELIUM; FEMALE; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HUMANS; KERATINOCYTES; MODELS, ANIMAL; SUSPENSIONS; SWINE; TRANSPLANTATION, HOMOLOGOUS; WOUND HEALING;

EID: 34848919297     PISSN: 10671927     EISSN: 1524475X     Source Type: Journal    
DOI: 10.1111/j.1524-475X.2007.00272.x     Document Type: Article

References (28)

1. Bennett N, Schultz P. Growth factors and wound healing: biochemical properties of growth factors and their receptors. Am J Surg 1993 165 : 728 37.
2. Singer A, Clark RA. Mechanisms of disease: cutaneous wound healing. N Engl J Med 1999 341 : 738 46.
3. Robson MC, Mustoe TA, Hunt TK. The future of recombinant growth factors in wound healing. Am J Surg 1998 176 : 80 2S.
4. Steed DL. Modifying the wound healing response with exogenous growth factors. Clinics in Plast Surg 1998 25 : 397 405.
5. Fu X, Li X, Cheng B, Chen W, Sheng Z. Engineered growth factors and cutaneous wound healing: success and possible questions in the past ten years. Wound Repair Regen 2005 13 : 122 30.
6. Breuing K, Eriksson E, Liu OY, Miller DR. Healing of partial thickness porcine skin wounds in a liquid environment. J Surg Res 1992 52 : 50 8.
7. Eriksson E, Vranckx JJ. Wet wound healing: from laboratory to patient to Gene therapy. Am J Surg 2004 188 : 36 41.
8. Vranckx JJ, Yao F, Eriksson E. Gene transfer of growth factors for wound repair. In : Rovee D, Maibach H, editors. The epidermis in wound healing. Boca Raton, FL, USA : CRC Press, 2004 : 265 83.
9. Andree C, Swain WF, Page C, Macklin MD, Slama J, Eriksson E. In vivo transfer and expression of a human epidermal growth factor gene accelerates wound repair. Proc Natl Acad Sci USA 1994 91 : 1288 92.
10. Vranckx JJ, Yao F, Petrie N, Augustinova H, Hoeller D, Visovatti S, Slama J, Eriksson E. In vivo gene delivery of Ad-VEGF121 to full-thickness wounds in aged pigs results in high levels of VEGF expression but not in accelerated healing. Wound Repair Regen 2005 13 : 51 60.
11. Brown GL, Nanney LB, Griffen J, Cramer AB, Yancey JM, Curtsinger LJ, Holtzin L, Schultz GS, Jurkiewicz MJ, Lynch JB. Enhancement of wound healing by topical treatment with epidermal growth factor. N Engl J Med 1989 321 : 76 80.
12. Falanga V, Eaglestein WH, Bucalo B, Katz MH, Harris B, Carson P. Topical use of human recombinant epidermal growth factor in venous ulcers. J Dermatol Surg Oncol 1992 18 : 604 6.
13. Rumalla VK, Borah GL. Cytokines, growth factors and plastic surgery. Plast Reconstr Surg 2001 108 : 719 24.
14. Martin P. Wound healing - aiming for perfect skin regeneration. Science 1997 276 : 75 81.
15. Svensjo T, Yao F, Pomahac B, Eriksson E. Autogenic keratinocyte suspensions accelerate epidermal wound healing in pigs. J Surg Res 2001 99 : 211 21.
16. Hunt JL, Purdue GF, Pownell P, Rohrich R. Burns: acute burns, burn surgery and post-burn reconstruction. Select Read Plast Surg 1997 8 : 1 37.
17. Cuono C, Langdon R, Mc Guire J. Use of cultured epidermal autografts and dermal allografts as skin replacement after burn injury. Lancet 1986 1 : 1123 4.
18. De Luca M, Albanese E, Bondanza S, Megna M, Ugozzoli L, Molina F, Cancedda R, Santi PL, Bormioli M, Stella M, et al. Multicentre experience in the treatment of burns with autogenic and allogenic cultured epithelium, fresh or preserved in a frozen state. Burns 1989 15 : 303 9.
19. Hefton JM, Madden MR, Finkelstein JL, Shires GT. Grafting of burn patients with allografts of cultured epidermal cells. Lancet 1983 2 : 428 30.
20. Aubock J, Irschick E, Romani N, Kompatscher P, Hopfl R, Herold M, Schuler G, Fritsch P. Rejection, after a slightly prolonged survival time, of Langerhans cell-free allogenic cultured epidermis used for wound coverage in humans. Transplantation 1988 45 : 730 7.
21. Rosenberg AS. Cellular basis of skin allograft rejection: an in vivo model of immune-mediated tissue destruction. Ann Rev Immunol 1992 10 : 333 7.
22. Morhenn VB, Benike CJ, Cox AJ, Charron DJ, Engleman EG. Cultured human epidermal cells do not synthesize HLA-DR. J Invest Dermatol 1982 78 : 32 6.
23. Dierch A, Chan SH, Benoist C, Mathis D. Graft rejection by T cells not restricted by conventional major histocompatibility complex molecules. Eur J Immunol 1993 23 : 2725 8.
24. Hunt JP, Hunter CT, Brownstein M, Hultman C, deSerres S, Bracey L, Frelinger J, Meyer A. Host priming, not target antigen type, decides rejection rate in mice primed with MHC-II "knock-out" cultured keratinocytes. J Surg Research 1998 76 : 32 6.
25. Thivolet J, Faure M, Demidem A, Mauduit G. Long-term survival and immunological tolerance of human epidermal allografts produced in culture. Transplantation 1985 42 : 274 80.
26. Rouabhia M. Permanent skin replacement using chimeric cultured sheets comprising xenogeneic and syngeneic keratinocytes. Transplantation 1996 61 : 1290 300.
27. Eriksson E, Yao F, Svensjo T, Winkler T, Slama J, Macklin M, Andree C, mc Gregor M, Hinshaw V, Swain W. In vivo gene transfer to skin by microseeding. J Surg Res 1998 78 : 85 91.
28. Vogt PM, Thompson S, Andree C, Liu P, Breuing K, Hatzis D, Mulligan RC, Eriksson E. Genetically modified keratinocytes transplanted to wounds reconstitute the epidermis. Proc Natl Acad Sci USA 1994 91 : 9307 11.