Unraveling multistate unfolding of pig kidney fructose-1,6-bisphosphatase using single tryptophan mutants

FEBS Journal

Volumn 274, Issue 20, 2007, Pages 5337-5349

  Ludwig, Heide C ^a   Pardo, Fabian N ^a   Asenjo, Joel L ^a   Maureira, Marco A ^a   Yañez, Alejandro J ^a   Slebe, Juan C ^a  

^a UNIVERSIDAD AUSTRAL DE CHILE   (Chile)

Author keywords

Fructose 1,6 bisphosphatase; Phase diagram; Protein unfolding; Single tryptophan mutants; Tetrameric intermediate

Indexed keywords

8 ANILINO 1 NAPHTHALENESULFONIC ACID; ACRYLAMIDE; ADENOSINE PHOSPHATE; FRUCTOSE BISPHOSPHATASE; GUANIDINE; MUTANT PROTEIN; TRYPTOPHAN;

ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ELECTROPHORETIC MOBILITY; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME KINETICS; FLUORESCENCE SPECTROSCOPY; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; KIDNEY; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE; SWINE; THERMODYNAMICS;

ANILINO NAPHTHALENESULFONATES; ANIMALS; CATALYSIS; CHROMATOGRAPHY, GEL; FRUCTOSE-BISPHOSPHATASE; GUANIDINE; KIDNEY; KINETICS; MAGNESIUM; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN RENATURATION; SPECTROMETRY, FLUORESCENCE; SULFHYDRYL COMPOUNDS; SULFHYDRYL REAGENTS; SWINE; TRYPTOPHAN;

SUIDAE;

EID: 34848907216     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2007.06059.x     Document Type: Article

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