Interactions of clinical and environmental Aeromonas isolates with Caco-2 and HT29 intestinal epithelial cells

Letters in Applied Microbiology

Volumn 45, Issue 4, 2007, Pages 405-410

  Couto, C R A ^a   Oliveira, S S ^b   Queiroz, M L P ^a   Freitas Almeida, A C ^a  

^a UNIVERSIDADE DO ESTADO DO RIO DE JANEIRO   (Brazil)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Adherence; Aeromonas spp.; Cellular viability; Cytotoxins production; Invasion

Indexed keywords

CELL CULTURE; CELLS; MONOLAYERS;

ADHERENCE; AEROMONAS; AEROMONAS SPP; AEROMONAS SPP.; CACO-2 CELLS; CELLULAR VIABILITY; CYTOTOXIN PRODUCTION; CYTOTOXINS; HT-29 CELLS; INVASION;

VEGETABLES;

TETRAZOLIUM;

CELLS; CYTOTOXICITY; DIFFUSION; PATHOGENS; SENSITIVITY ANALYSIS; STRAIN; VEHICLES;

BACTERIUM; PATHOGEN; PUBLIC HEALTH; VIRULENCE;

AEROMONAS; AEROMONAS HYDROPHILA; AEROMONAS PUNCTATA; ANIMAL CELL; ARTICLE; BACTERIAL STRAIN; BACTERIUM ADHERENCE; BACTERIUM ISOLATION; CELL STRAIN CACO 2; CELL STRAIN HT29; CELL TYPE; CONTROLLED STUDY; CYTOPATHOGENIC EFFECT; CYTOTOXICITY; DIARRHEA; FOOD ANALYSIS; HUMAN; HUMAN CELL; INTESTINE EPITHELIUM CELL; MOLECULAR INTERACTION; NONHUMAN; SENSITIVITY ANALYSIS; VEGETABLE; VERO CELL; WATER SAMPLING;

AEROMONAS; AEROMONAS HYDROPHILA; AEROMONAS PUNCTATA;

EID: 34848875692     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2007.02199.x     Document Type: Article

References (25)

1. Ágarwal, R.K., Kapoor, K.N. Kumar, A. (1998) Virulence factors of aeromonads - an emerging food borne pathogen problem. J Commun Dis 30, 71 78.
2. Araújo, V.S., Castro, T.L.d'A., Luna, M.G. Freitas-Almeida, A.C. (2002) Distribuition of enterotoxin/hemolysin gene among Aeromonas isolated from human and food. Seventh International Symposium on Aeromonas and Plesiomonas, Orihuela (Alicante) Spain.
3. Benitz, J.A., Spelbrink, R.G., Silva, A., Phillips, T.E., Stanley, M., Boesman-Finkelstein, M. Finkelstein, R.A. (1997) Adherence of Vibrio cholerae to cultured differentiated human intestinal cells: an in vitro colonization model. Am Soc Microbiol 65, 3474 3477.
4. Castro-Escarpulli, G., Figueiras, M.J., Aguilera-Arreola, G., Soler, L., Fernández-Rendón, E., Aparicio, G.O., Guarro, J. Chacón, M.R. (2003) Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico. Int J Food Microbiol 84, 41 49.
5. Chopra, A.K. Houston, C.W. (1999) Enterotoxins in Aeromonas-associated gastroenteritis. Microb Infect 1, 1129 1137.
6. Denizot, F. Lang, R. (1986) Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immun Methods 89, 271 277.
7. Freitas, A.C., Souza, S.M.S., Macedo, L.C., Pinto, E.C. Pereira, S.S. (1998) Aeromonas species associated with gastroenteritis in children: prevalence, characteristics and virulence properties. Rev Microbiol 29, 152 157.
8. Gavín, R., Merino, S., Atarriba, M., Canals, R., Shaw, J.G. Tomás, J.M. (2003) Lateral flagella are required for increased cell adherence, invasion and biofilm formation by Aeromonas spp. FEMS Microbiol Lett 224, 77 83.
9. Granum, P.E., O'Sullivan, K., Tomás, J.M. Ørmen, Ø. (1998) Possible virulence factors of Aeromonas sp. from food and water. FEMS Immunol Med Microbiol 21, 131 137.
10. Guimarães, M.S., Andrade, J.R.C., Freitas-Almeida, A.C. Ferreira, M.C.S. (2002) Aeromonas hydrophila vacuolating activity in the Caco-2 human enterocyte cell line as a putative virulence factor. FEMS Microbiol Lett 207, 127 131.
11. Harf-Monteil, C., Prèvost, G. Monteil, H. (2004) Facteurs de virulence d'Aeromonas caviae isoles de cas cliniques. Pathologie Biologie 52, 21 25.
12. Janda, J.M. Abbott, S.L. (1998) Evolving concepts regarding the genus Aeromonas: an expanding panorama of species, disease presentations and unanswered questions. Clin Infect Dis 27, 332 344.
13. Joseph, S.W. Carnahan, A.M. (2000) Update on the genus Aeromonas. ASM News 66, 218 223.
14. Kirov, S.M. (1997) Aeromonas and Plesiomonas species. In Food Microbiology, Fundamentals and Frontiers ed. Doyle, M.P., Beuchat, L.R. Monteville, T.J. Washington DC : ASM Press.
15. Kirov, S.M., Hayward, L.J. Nerrie, M.A. (1995) Adhesion of Aeromonas sp. to cell lines used as model for intestinal adhesion. Epidemiol Infect 115, 465 473.
16. Kreft, B., Carstensen, O., Straube, E., Bohnet, S., Hacker, J. Marre, R. (1992) Adherence to and cytotoxicity of Escherichia coli for eucaryotic cell lines quantified by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide). Zbl Bakt 276, 231 242.
17. Krovaceck, K., Pasquale, V., Balada, S.B., Soprano, V., Conte, M. Dumontet, S. (1994) Comparison of putative virulence factors in Aeromonas hydrophila strains isolated from the marine environment and human diarrhoeal cases in southern Italy. Appl Environ Microbiol 60, 1379 1382.
18. Lawson, M.A., Burke, V. Change, B.J. (1985) Invasion of HEp-2 cells by fecal isolates of Aeromonas hydrophila. Infect Immun 47, 680 683.
19. Martins, L.M., Marquez, R.F. Yano, T. (2002) Incidence of toxic Aeromonas isolated from food and human infection. FEMS Immun Med Microbiol 32, 237 242.
20. Mekalanos, J.J. (1990) Environmental signals controlling expression of virulence determinants in bacteria. J Bacteriol 174, 1 7.
21. Neves, M.S., Nunes, M.P. Milhomem, A.M. (1994) Aeromonas species exhibit aggregative adherence to HEp-2 cells. J Clin Microbiol 32, 1130 1131.
22. Palú, A.P., Gomes, L.M., Miguel, M.A.L., Balassiano, I.T., Queiroz, M.L.P., Freitas-Almeida, A.C. Oliveira, S.S. (2006) Antimicrobial resistance in food and clinical Aeromonas isolates. Food Microbiol 23, 504 509.
23. Rocha-de-Souza, C.M., Mattos-Guaraldi, A.L., Hirata, R.J., Moreira, L.O., Monteiro-leal, L.H., Freitas-Almeida, A.C., Previato, L.M., Previato, J.O., et al. (2003) Influence of polarisation and differentiation on interaction of 43-kDa outer-membrane protein of Aeromonas caviae with human enterocyte-like Caco-2 cell line. Int J Mol Med 11, 661 667.
24. Thorley, J.P., Shaw, J.G., Gryllos, I.A. Eley, A. (1996) Adherence of Aeromonas caviae to human cell lines HEp-2 and Caco-2. J Med Microbiol 45, 445 451.
25. Trajano, R.S., Pinto, E.C., Reis, L.G., Oliveira, S.S., Vanweerelt, M., Sanchez-Nunez, M.L., Taketani, R.G., Araújo, F.V., et al. (2003) Detecção de cepas multi-resistentes de Aeromonas spp. e análise do índice de poluição fecal de águas superficiais da lagoa Rodrigo de Freitas, Rio de Janeiro, Brasil. 22° Congresso Brasileiro de Microbiologia Florianópolis (Santa Catarina), Brasil.