Specific detection of Peronospora tabacina by PCR-amplified rDNA sequences

Plant Pathology Journal

Volumn 5, Issue 3, 2006, Pages 378-382

  Tsay, Jwu Guh ^a   Chu, Chishih ^a   Chuang, Ya Han ^a   Chen, Ruey Shyang ^a  

^a NATIONAL CHIAYI UNIVERSITY   (Taiwan)

Author keywords

Downy mildew; Internal transcribed spacer; Nested PCR; Tobacco

Indexed keywords

FUNGI; NICOTIANA TABACUM; PERONOSPORA; PERONOSPORA TABACINA; PERONOSPORACEAE;

EID: 34848868122     PISSN: 18125387     EISSN: 18125425     Source Type: Journal    
DOI: 10.3923/ppj.2006.378.382     Document Type: Article

References (17)

1. Agrios, G.N., 1997. Plant Pathology. 4th Edn., Academic Press, New York, pp: 635.
2. Chen, R.S. and D.D. Tzeng, 1999. PCR-mediated detection of Ustilago esculenta in wateroat (Zizania latifolia) by ribosomal internal transcribed spacer sequences. Plant Pathol. Bull., 8: 149-151.
3. Edreva, A., R. Delon and J. Coussirat, 1998. Variability of P. tabacina A. An isoenzyme study. Beitr. Tabakforsch. Intl., 18: 3-13.
4. Lai, P.S., T.J. Lien, R.S. Chen and J.G. Tsay, 2004. The occurrence of downy mildew of vegetable soybean and its detection by PCR. Plant Prot. Bull., 46: 155-162.
5. Lee, S.B. and J.W. Taylor, 1992. Phylogeny of five fungus-like protoctistan Phytophthora species, inferred from the internal transcribed spacers of ribosomal DNA. Mol. Biol. Evol., 9: 636-653.
6. Lucas, G.B., 1980. The war against blue mold. Science, 210: v147-153.
7. Matsumoto, C., K. Kageyama, H. Suga and M. Hyakumachi, 1999. Phylogenetic relationships of Pythium species based on ITS and 5.8S sequences of the ribosomal DNA. Mycoscience, 40: 321-331.
8. McKeen, W.E., 1989. Blue Mold of Tobacco. APS press. St. Paul, Minn, USA., pp: 288.
9. Tsai, Y.P., 1991. List of Plant Diseases in Taiwan 3rd Edn. The Plant Protection Society, Taiwan, R.O.C. pp: 604 (in Chinese)
10. Wang, P.H. and J.G. White, 1997. Molecular characterization of Pythium species based on RFLP analysis of the internal transcribed spacer region of ribosomal DNA. Physiol. Mol. Plant Pathol., 51: 129-143.
11. Wang, P.H. and C.W. Chang, 2003. Detection of the low-germination-rate resting oospores of Pythium myriotylum from soil by PCR. Lett. Applied., Microbiol., 36: 157-161.
12. Wang, P.H., Y.T. Wang and J.G. White, 2003. Species-specific PCR primers for Pythium developed from ribosomal ITS1 region. Lett. Applied Microbiol., 37: 127-132.
13. White, T.J., T. Bruns, S. Lee and J. Taylor, 1990. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In: PCR Protocols: A Guide to Methods and Applications. Innis M.A., D.V. Gelfand, J.J. Sninsky and T.J. White (Eds.), Academic Press, New York pp: 315-322.
14. Wiglesworth, M.D., C.L. Schardl, W.C. Nesmith and M.R. Siegel, 1991. Differentiation of peronosporales and isolates of Peronospora tabacina by direct sequencing of an internal transcribed spacer (ITS2). Phytopathology, 81: 1237.
15. Wiglesworth, M.D., 1994. Differentiation and detection of Peronospora tabacina Adam. using the polymerase chain reaction. PhD Thesis, University of Kentucky, Lexington.
16. Sukno, S.A., A.M. Taylor and M.L. Farman, 2002a. Development of contamination-free restriction fragment length polymorphism probes for the obligate biotroph Peronospora tabacina, an oomycete causing blue mold of tobacco. Phytopathology, 92: 1227-1235.
17. Sukno, S.A., A.M. Taylor and M.L. Farman. 2002b. Genetic uniformity among isolates of Peronospora tabacina, the tobacco blue mold pathogen. Phytopathology, 92:1236-1244.