Addition of exogenous reporter peptides to serum samples before mass spectrometry-based protease profiling provides advantages over profiling of endogenous peptides [1]

Clinical Chemistry

Volumn 53, Issue 10, 2007, Pages 1864-1866

  Findeisen, Peter ^a   Post, Stefan ^a   Wenz, Frederik ^a   Neumaier, Michael ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

APC PROTEIN; BIOLOGICAL MARKER; ORGANIC SOLVENT; PLASMA PROTEIN; PROTEINASE; RECOMBINANT PROTEIN; TRYPSIN;

CHROMATOGRAPHY; HUMAN; HUMAN TISSUE; LETTER; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PROTEIN DEGRADATION; PROTEIN PURIFICATION; PROTEOMICS; REPRODUCIBILITY; SERUM; STANDARDIZATION; THROMBOTIC THROMBOCYTOPENIC PURPURA;

ADENOMATOUS POLYPOSIS COLI PROTEIN; HUMANS; PEPTIDE FRAGMENTS; PEPTIDE HYDROLASES; PROTEOMICS; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SERUM; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 34848866288     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2006.083030     Document Type: Letter

References (5)

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2. Karsan A, Eigl BJ, Flibotte S, Gelmon K, Switzer P, Hassell P, et al. Analytical and preanalytical biases in serum proteomic pattern analysis for breast cancer diagnosis. Clin Chem 2005;51:1525-8.
3. Hortin GL. The MALDI-TOF mass spectrometry view of the plasma proteome and peptidome. Clin Chem 2006;52:1223-37.
4. Villanueva J, Shaffer DR, Philip J, Chaparro CA, Erdjument-Bromage H, Olshen AB, et al. Differential exoprotease activities confer tumor-specific serum peptidome patterns. J Clin Invest 2006;116:271-84.
5. Jin M, Cataland S, Bissell M, Wu HM. A rapid test for the diagnosis of thrombotic thrombocytopenic purpura using surface enhanced laser desorption/ionization time-of-flight (SELDI-TOF)-mass spectrometry. J Thromb Haemost 2006;4:333-8.