Direct nested RT-PCR for the detection of Plum pox virus

Acta Horticulturae

Volumn 657, Issue , 2004, Pages 155-158

  Varveri, C ^a  

^a BENAKI PHYTOPATHOLOGICAL INSTITUTE   (Greece)

Author keywords

Crude extracts; Diagnosis; PPV

Indexed keywords

EID: 34748814655     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/ActaHortic.2004.657.20     Document Type: Conference Paper

References (9)

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3. Olmos, A., Cambra, M., Dasi, M.A., Candresse, T., Esteban, O., Gorris, M.T. and Asensio, M. 1997. Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by Heminested-PCR and PCR-ELISA. J. Virol. Methods 68: 127-137.
4. Olmos, A., Cambra, M., Esteban, O., Gorris, M.T. and Terrada, E. 1999. New device and method for capture, reverse transcription and nested PCR in a single closed-tube. Nucleic Acids Res. 27: 1564-1565.
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7. Varveri, C. and Boutsika, K. 1998. Application of the immunocapture-PCR technique for plum pox potyvirus detection under field conditions in Greece and assays to simplify standard techniques. Acta Hort 472: 475-481.
8. Varveri, C. and Boutsika, K. 1999. Application of a simple IC/PC-PCR-ELISA technique for plum pox potyvirus detection. Petria 9: 137-140.
9. Wetzel, T., Candresse, T., Macquaire, G., Ravelonandro, M. and Dunez, J. 1992. A highly sensitive immunocapture polymerase reaction method for plum pox potyvirus detection. J. Virol. Methods 39: 27-37.