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34648836247
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note
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4OS.
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4O.
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34648833167
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note
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7b.
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34648830761
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note
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Open-field test. A total of 84 adult CPU mice males (30-40 g) were divided into 14 experimental groups (each consisting of 6 animals). Six groups were used to evaluate the effects of test compounds on spontaneous locomotor activity. Compounds 8a and 9a were originally dissolved in DMSO (final conc. up to 2%), diluted with saline, and ip injected (1.0 mg, or 10.0 mg in 2.0 ml per kg bw) 20 min before another injection of saline. The controls received first saline/DMSO and the comparison drug's group was primary treated with clozapine (5 mg/kg). Remaining eight groups of mice were employed to assess the effects of the first treatment on amphetamine-induced hyperactivity. They received (by the same schedule as above) either 1.0 mg, 4.0 mg, or 10.0 mg of 8a or 9a, saline/DMSO or clozapine (5 mg/kg), 20 min prior to d-AMPH sulphate (3.0 mg/kg). Each animal was positioned in the center of the plastic open-box (40 × 40 × 30 cm) immediately after the second injection and for the next 15 min they were allowed to adapt to the environment. The test room with the acoustic isolation was dimly illuminated (indirect 2 × 40 W light). During the following 60 min interval, locomotion was registered by a web-camera connected to PC and controlled by ANY-maze software (ANY-maze Video Tracking System 4.30, Stoelting Co., USA). The same program was also used to calculate two parameters chosen to illustrate total animal locomotion. (1) Time of activity (in seconds) was the sum of periods when animals moved in space. (2) Total head distance (in meters) demonstrated both the total locomotion in space and the head movements made in place, also representing a measure of some stereotype behaviors. Crude data was analyzed by GraphPad Prysm (4.00) using one-way ANOVA followed by Bonferroni's multiple group comparison test.
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0033777513
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Svensson, T.H.J.6
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0042887023
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Shapiro D.A., Renock S., Arrington E., Chiodo L.A., Liu L.X., Sibley D.R., Roth B.L., and Mailman R. Neuropsychopharmacology 28 (2003) 1400
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Sibley, D.R.6
Roth, B.L.7
Mailman, R.8
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