Design and evaluation of analogues of the bacterial cell-wall peptidoglycan motif l-Lys-d-Ala-d-Ala for use in a vancomycin biosensor

Bioorganic and Medicinal Chemistry Letters

Volumn 17, Issue 21, 2007, Pages 5758-5762

  Hernout, Olivier ^a   Berthoin, Karine ^a   Delattre, Isabelle ^a   Tulkens, Paul M ^a   Carryn, Stéphane ^a,b   Marchand Brynaert, Jacqueline ^a  

^a UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

^b Eumedica SA   (Belgium)

Author keywords

Biosensor; d Ala d Ala derivatives; Microbiological assay; Vancomycin

Indexed keywords

AMINOCAPROIC ACID DERIVATIVE; LYSYLALANYLALANINE; N ACETYL 6 AMINOCAPROYL DEXTRO ALANINE DEXTRO ALANINE; N ACETYL 6 AMINOCAPROYL DEXTRO ALANINE DEXTRO SERINE; N BUTYLOXYCARBONYL 6 AMINOCAPROYL DEXTRO ALANINE DEXTRO ALANINE; PEPTIDOGLYCAN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL WALL; BIOSENSOR; CONCENTRATION RESPONSE; DEVICE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INFRARED SPECTROSCOPY; NONHUMAN;

BACTERIA; BIOSENSING TECHNIQUES; CELL WALL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DRUG DESIGN; DRUG EVALUATION, PRECLINICAL; PEPTIDOGLYCAN; SPECTROPHOTOMETRY, ULTRAVIOLET; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; VANCOMYCIN;

BACTERIA (MICROORGANISMS);

EID: 34648826035     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2007.08.055     Document Type: Article

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21. The ordinate shows the variation in the number of viable bacteria after 5 h of incubation compared to the original inoculum (horizontal dotted line). Negative values indicate bacterial killing due to the action of vancomycin (the values of the negative plateaus are similar to what was observed with vancomycin alone [not shown]). Positive values correspond to bacterial growth due to the decreased efficacy of vancomycin in the presence of the tested compounds (the values of the positive plateaus are similar to what was observed in the absence of vancomycin [not shown]. Curves were generated by fitting a variable slope sigmoidal dose-response equation using GrapPad Prism (version 4.03, GraphPad Software Inc., San Diego, CA) to the experimental points. EC50 values (shown on Table 1) and slope factors (spanning from 2.83 to 3.92) were calculated with the same software for each compound. Each point represents the arithmetic mean of triplicates ±SD and the experiment was repeated twice with similar results.
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