Heterologous expression of the kanamycin biosynthetic gene cluster (pSKC2) in Streptomyces venezuelae YJ003

Applied Microbiology and Biotechnology

Volumn 76, Issue 6, 2007, Pages 1357-1364

  Thapa, Laxmi Prasad ^a   Oh, Tae Jin ^a   Lee, Hei Chan ^a   Liou, Kwangkyoung ^a   Park, Je Won ^b   Yoon, Yeo Joon ^b   Sohng, Jae Kyung ^a  

^a SunMoon University   (South Korea)

^b Ewha Womans University   (South Korea)

Author keywords

Heterologous expression; Kanamycin; Streptomyces kanamyceticus ATCC 12853; Streptomyces venezuelae YJ003

Indexed keywords

ANTIBIOTICS; BIOSYNTHESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IONIZATION; MASS SPECTROMETRY;

HETEROLOGOUS EXPRESSION; KANAMYCIN; STREPTOMYCES KANAMYCETICUS ATCC 12853; STREPTOMYCES VENEZUELAE YJ003;

GENETIC ENGINEERING;

AMINOGLYCOSIDE ANTIBIOTIC AGENT; KANAMYCIN; KANAMYCIN A;

ANTIBIOTICS; BACTERIUM; GENE; IONIZATION; MASS SPECTROMETRY;

ARTICLE; BACTERIAL GROWTH; BACTERIUM ISOLATE; BACTERIUM TRANSFORMATION; BIOSYNTHESIS; ELECTROSPRAY MASS SPECTROMETRY; GENE CLUSTER; GENE EXPRESSION; GENETIC RESISTANCE; HETEROLOGOUS EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; REGULATOR GENE; STREPTOMYCES VENEZUELAE; TANDEM MASS SPECTROMETRY;

BACTERIAL PROTEINS; CARBOHYDRATE SEQUENCE; GENE EXPRESSION REGULATION, BACTERIAL; KANAMYCIN; MULTIGENE FAMILY; STREPTOMYCES;

STREPTOMYCES KANAMYCETICUS; STREPTOMYCES VENEZUELAE;

EID: 34648813081     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-1096-4     Document Type: Article

References (15)

1. Baud H, Betencourt A, Peyre M, Penasse L (1977) Ribostamycin, as an intermediate in the biosynthesis of neomycin. J Antibiot 30:720-723
2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
3. Eustaquio AS, Gust B, Galm U, Li SM, Chater KF, Heide L (2005) Heterologous expression of novobiocin and clorobiocin biosynthetic gene clusters. Appl Environ Microbiol 71:2452-2459
4. Hong JS, Park SH, Choi CY, Sohng JK, Yoon YJ (2004) New olivosyl-derivatives of methymycin/pikromycin from an engineered strain of Streptomyces venezuelae. FEMS Microbiol Lett 238:391-399
5. Huang F, Haydock SF, Mironenko T, Spiteller D, Li Y, Spencer JB (2005) The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine. Org Biomol Chem 3:1410-1418
6. Kharel MK, Basnet BD, Lee HC, Liou K, Woo JS, Sohng JK (2003a) Isolation and characterization of the tobramycin biosynthetic gene cluster from Streptomyces tenebrarius. FEMS Microbiol Lett 230:185-190
7. Kharel MK, Subba B, Lee HC, Liou K, Woo JS, Sohng JK (2003b) Identification of 2-deoxy-scyllo-inosose synthase in aminoglycoside producer Streptomyces. J Microbiol Biotechnol 13:828-831
8. Kharel MK, Subba B, Basnet BD, Woo JS, Lee HC, Sohng JK (2004) A gene cluster for biosynthesis of kanamycin from Streptomyces kanamyceticus: comparison with gentamicin biosynthetic gene cluster. Arch Biochem Biophys 429:204-214
9. Kharel MK, Subba B, Lee HC, Liou K, Sohng JK (2005) Characterization of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase (tbmB) from Streptomyces tenebrarius. Bioorg Med Chem Lett 15:89-92
10. Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA (2000) Practical streptomyces genetics. John Innes Foundation, Norwich
11. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
12. Stead DA, Richards RM (1996) Sensitive fluorimetric determination of gentamicin sulfate in biological matrices using solid-phase extraction, pre-column derivatization with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography. J Chromatogr B Biomed Appl 675:295-302
13. Stead DA, Richards RM (1997) Sensitive high-performance liquid chromatographic assay for aminoglycosides in biological matrices enables the direct estimation of bacterial drug uptake. J Chromatogr, B, Biomed Sci Appl 693:415-421
14. Sztaricskai F (2001) The fight against bacteria: the antibiotics policy. Acta Pharm Hung 71:89-98
15. Xue Y, Zhao L, Liu HW, Sherman DH (1998) A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: Architecture of metabolic diversity. Proc Natl Acad Sci USA 95:12111-12116