Long-term primary culture of secretory cells of Bothrops jararaca venom gland for venom production in vitro

Nature Protocols

Volumn 1, Issue 6, 2007, Pages 2763-2766

  Yamanouye, Norma ^a   Kerchove, Celine Marie ^a   Moura da Silva, Ana Maria ^a   Carneiro, Sylvia M ^a   Markus, Regina P ^b  

^a INSTITUTO BUTANTAN   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

SNAKE VENOM;

ANIMAL; ARTICLE; BIOSYNTHESIS; CULTURE TECHNIQUE; CYTOLOGY; EXOCRINE GLAND; MOUSE; SECRETION; SNAKE;

ANIMALS; BOTHROPS; CELL CULTURE TECHNIQUES; CROTALID VENOMS; EXOCRINE GLANDS; MICE;

EID: 34548862300     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.423     Document Type: Article

References (13)

1. Carneiro, S.M. et al. Venom production in long-term primary culture of secretory cells of the Bothrops jararaca venom gland. Toxicon 47, 87-94 (2006).
2. Laing, G.D. & Moura-da-Silva, A.M. Jararhagin and its multiple effects on hemostasis. Toxicon 45, 987-996 (2005).
3. Quissell, D.O., Redman, R.S. & Mark, M.R. Short-term primary culture of acinar-intercalated duct complexes from rat submandibular glands. In Vitro Cell. Dev. Biol. 22, 469-480 (1986).
4. Redman, R.S., Quissell, D.O. & Barzen, K.A. Effects of dexamethasone, epidermal growth factor, and retinoic acid on rat submandibular acinarintercalated duct complexs in primary culture. In Vitro Cell. Dev. Biol. 24, 734-742 (1988).
5. Kochva, E. The origin of snakes and evolution of the venom gland apparatus. Toxicon 25, 65-106 (1987).
6. Belluomini, H.E. In Venomous Animals and Their Venoms Vol. 1 (eds. Bucherl, W., Buckley, V. & Deulofeu, V.) 97-117 (Academic Press, New York, 1968).
7. Devi, A. The protein and nonprotein constituents of snake venoms. In Venomous Animals and Their Venoms Vol. 1 (eds. Bucherl, W., Buckley, V. & Deulofeu, V.) 119-165 (Academic Press, New York, USA, 1968).
8. Carneiro, S.M., Fernandes, W., Sant'anna, S.S. & Yamanouye, N. Microvesicles in the venom of Crotalus durissus terrificus (Serpente, Viperidae). Toxicon 47, 106-110 (2007).
9. Duarte, M.M., De Oca, H.M., Diniz, C.R. & Fortes-Dias, C.L. Primary culture of venom gland cells from the South American rattlesnake (Crotalus durissus terrificus). Toxicon 37, 1673-1682 (1999).
10. Golubkov, V.S., Lezhnerv, E.L., Bezgina, E.N. & Moshkov, D.A. Primary culture of epithelial secretory cells from venom gland of the common adder Vipera berus. Tsitologiia 43, 453-461 (2001).
11. Sells, P.G., Hommel, M. & Theakston, R.D.G. Venom production in snake venom gland cells cultured in vitro. Toxicon 27, 1245-1249 (1989).
12. Theakston, R.D.G. & Reid, H.A. Development of simple assay procedures for the characterisation of snake venoms. Bull. World Health Organ. 61, 949-956 (1983).
13. Laing, G.D., Clissa, P.B., Theakston, R.D.G., Moura-da-Silva, A.M. & Taylor, M.J. Inflammatory pathogenesis of snake venom metalloproteinase-induced skin necrosis. Eur. J. Immunol. 33 3458-3463 (2003).