Novel DNA staining method and processing technique for the quantification of undamaged double-stranded DNA in epidermal tissue sections by PicoGreen probe staining and microspectrophotometry

Journal of Histochemistry and Cytochemistry

Volumn 55, Issue 10, 2007, Pages 999-1014

  Gagna, Claude E ^a,b,c   Kuo, Hon Reen ^a   Chan, Norman J ^b   Mitacek, Eugene J ^b   Spivak, Alia ^b   Pasquariello, Tiffany D ^b   Balgobin, Chandrika ^b   Mukhi, Ruhayna ^b   Lambert, W Clark ^a  

^a RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

^b NEW YORK INSTITUTE OF TECHNOLOGY   (United States)

^c NEW YORK INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

B DNA; DNA quantification; DNA structure; Double stranded DNA; Fixatives; Histochemistry; Histotechnology; PicoGreen; Tissue processing; Z DNA

Indexed keywords

DOUBLE STRANDED DNA; FIXATIVE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DNA CONTENT; DNA DAMAGE; DNA DENATURATION; DNA DETERMINATION; DNA PROBE; DNA STRUCTURE; EPIDERMIS; HEATING; HIGH TEMPERATURE; HISTOCHEMISTRY; MICROSPECTROPHOTOMETRY; MICROWAVE RADIATION; NONHUMAN; PICOGREEN STAINING; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; STAINING; SWINE; TISSUE FIXATION; VACUUM;

ANIMALS; DNA; EPIDERMIS; FIXATIVES; FLUORESCENT DYES; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; IMMUNOHISTOCHEMISTRY; NUCLEIC ACID CONFORMATION; NUCLEIC ACID DENATURATION; ORGANIC CHEMICALS; PARAFFIN EMBEDDING; PLOIDIES; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; SWINE;

EID: 34548768827     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.7A7194.2007     Document Type: Article

References (46)

1. Ahn SJ, Costa J, Emanuel JR (1996) PicoGreen quantitation of DNA: effective evaluation of samples pre- or post PCR. Nucleic Acids Res 24:2623-2625
2. Al-Gubory KH (2005) Fibered confocal fluorescence microscopy for imaging apoptotic DNA fragmentation at the single-cell level in vivo. Exp Cell Res 310:474-481
3. Bowtell D, Sambrook J (2003) DNA Microarrays: A Molecular Cloning Manual. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press
4. Bramwell NH, Burns BF (1988) The effects of fixative type and fixation time on the quantity and quality of extractable DNA for hybridization studies on lymphoid tissue. Exp Hematol 16:730-732
5. Brutlag D, Schlehuber C, Bonner J (1969) Properties of formaldehyde-treated nucleohistone. Biochemistry 8:3214-3218
6. Carson FL (1997) Histotechnology: A Self-Instructional Text. Chicago, ASCP Press
7. Conn MP, ed. (1990) Methods in Neurosciences, vol. 3, Quantitative and qualitative microscopy. San Diego, Academic Press
8. Cross SS, Start RD, Smith JH (1990) Does delay in fixation affect the number of mitotic figures in processed tissue? J Clin Pathol 43: 597-599
9. Douglas MP, Rogers SO (1998) DNA damage caused by common cytological fixatives. Mutat Res 401:77-88
10. Gagna CE, Lambert WC, Kuo H-R, Farnsworth PN (1997) Localization of B-DNA and Z-DNA in terminally differentiating fiber cells in the adult lens. J Histochem Cytochem 45:1511-1521
11. Giberson RT, Demaree RS, eds. (2001) Microwave: Techniques and Protocols. Totowa, NJ, Humana Press
12. Gillett R, Gull K (1972) Glutaraldehyde - its purity and stability. Histochemie 30:162-167
13. Guillo C, Ferrance JP, Landers JP (2006) Use of capillary electrophoresis instrument with laser-induced fluorescence detection for DNA quantitation comparison of YO-PRO-1 and PicoGreen assays. J Chromatogr A 1113:239-243
14. Hames BD, Higgins SJ (1995) Gene Probes 1: A Practical Approach. Oxford, IRL Press
15. Haugland RP, ed. (2002) Nucleic acid detection and genomics technology. In Handbook of Fluorescent Probes and Research Products. Eugene, OR, Molecular Probes Press, 265-352
16. Henwood AF (2005) Effect of slide drying at 80°C on immunohistochemistry. J Histotechnol 28:45-46
17. Herbert A, Rich A (1999) Left-handed Z-DNA: structure and function. Genetica 106:37-47
18. Hobot JA, Newman GR (1991) Strategies for improving the cytochemical and immunocytochemical sensitivity of ultrastructurally well-preserved, resin embedded biological tissue for light and electron microscopy. In Roomans G, Edelmann L, eds. Scanning Microscopy Supplement 5. Scanning Microscopy International, Chicago, AMF O'Hare, S27-41
19. Hopwood D (1970) The reactions between formaldehyde, glutaraldehyde and osmium tetroxide and their fixation effects on bovine serum albumin and tissue blocks. Histochem J 24:56-64
20. Huang SN, Minassian H, More JD (1976) Application of immunofluorescent staining on paraffin sections improved by trypsin digestion. Lab Invest 35:383-390
21. Jamur MC, Faraco CD, Lunardi LO, Siraganian RP, Oliver C (1995) Microwave fixation improves antigenicity of glutaraldehyde-sensitive antigens while preserving ultrastructural detail. J Histochem Cytochem 43:307-311
22. Keller GH, Manak DM, eds. (1993) DNA Probes. New York, Stockton Press
23. Kiernan JA, Mason I (1996) Microscopy and Histology for Molecular Biologists: A User Guide. Oxford, Portland Press
24. Kingsbury AE, Foster OJ, Nisbet AP, Cairns N, Bray L, Eve DJ, Lees AJ, et al. (1995) Tissue pH as an indicator of mRNA preservation in human post-mortem brain. Brain Res Mol Brain Res 28:311-318
25. Kok LP, Boon ME (1992) Microwave Cookbook for Microscopists: Art and Science of Visualization. Leiden, The Netherlands, Coulomb Press Leyden
26. Kowalski D, Kroeker WD, Laskowski M (1976) Mung bean nuclease I. Physical, chemical, and catalytic properties. Biochemistry 15:4457-4463
27. Kral T, Widerak K, Langner M, Hof M (2005) Propidium iodide and PicoGreen as dyes for the DNA fluorescence correlation spectroscopy measurements. J Fluoresc 15:179-183
28. Kuo HR, Lapidus A, Lambert WC (1998) Identification of early apoptosis in Feulgen-stained cultured cells in situ by computerized image analysis. Cytometry 33:420-427
29. Labarca C, Paigen K (1980) A simple, rapid and sensitive DNA assay procedure. Anal Biochem 102:344-352
30. LePecq JB, Paloetti C (1967) A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. J Mol Biol 27:87-106
31. Marani E, Feirabend HK, Ploeger S (1996) The search for vacuo-microwave technique: the vacuum-microwave oven. Eur J Morphol 34:123-130
32. Marmur J, Doty P (1961) Thermal renaturation of deoxyribonucleic acids. J Mol Biol 3:585-594
33. Murakami P, McCaman MT (1999) Quantitation of adenovirus DNA and virus particles with the PicoGreen Fluorescent Dye. Anal Biochem 274:283-288
34. Pomppanen E-L, Savolainen K, Mononen I (2000) Optimal use of the fluorescent PicoGreen dye for quantitative analysis of amplified polymerase chain reaction products on microplate. Anal Biochem 279:111-114
35. Poulin N, Harrison A, Palcic B (1994) Quantitative precision of an automated image cytometric system for the measurement of DNA content and distribution in cells labeled with fluorescent nucleic acid stains. Cytometry 16:227-235
36. Rengarajan K, Cristol SM, Mehta M, Nickerson JM (2002) Quantifying DNA concentrations using fluorometry: a comparison of fluorophores. Mol Vis 8:416-421
37. Rich A, Zhang S (2003) Timeline: Z-DNA: the long road to biological function. Nat Rev Genet 4:566-572
38. Rosenthal SL (1987) A review of the mutagenicity of chloroform. Environ Mol Mutagen 10:211-226
39. Roth J, Ziak M, Guhl B (2000) Non-heating antigen retrieval techniques for light and electron microscopic immunolabelling. In Shi SR, Gu J, Taylor CR, eds. Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology. Natick, MA, Easton Publishing, 275-285
40. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press
41. Serth J, Kuczyk MA, Paeslack U, Lichtinghagen R, Jonas U (2000) Quantitation of DNA extracted after micropreparation of cells from frozen and formalin-fixed tissue sections. Am J Pathol 156: 1189-1196
42. Shi S-R, Gu J, Taylor CR, eds. (2000) Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology. Westborough, MA, BioTechniques Press
43. Singer VL, Jones LJ, Yue ST, Haugland RP (1997) Characterization of PicoGreen reagent and development of a fluorescence-based solution assay for double-stranded DNA quantitation. Anal Biochem 249:228-238
44. Srinivasan M, Sedmak D, Jewell S (2002) Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol 161:1961-1971
45. Vogt VM (1980) Purification and properties of S1 nuclease from Aspergillus. Methods Enzymol 65:248-255
46. Waring MJ (1965) Complex formation between ethidium bromide and nucleic acids. J Mol Biol 13:269-282