A new triple staining method for double in situ hybridization in combination with cell lineage tracing in whole-mount Xenopus embryos

Development Growth and Differentiation

Volumn 49, Issue 8, 2007, Pages 635-645

  Koga, Masaaki ^a   Kudoh, Tomohiro ^a   Hamada, Yuki ^a   Watanabe, Masashi ^a   Kageura, Hiroshi ^a  

^a FUKUOKA UNIVERSITY   (Japan)

Author keywords

Bleach; Cell lineage tracing; Double in situ hybridization; Triple staining; Xenopus

Indexed keywords

GENE PRODUCT; PIGMENT; PROTEIN GSC; PROTEIN XBRA; TRACER; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BLASTOMERE; BLEACHING; CELL DIFFERENTIATION; CELL INTERACTION; CELL LABELING; CELL LINEAGE; CELL TRANSPLANTATION; CONTRAST ENHANCEMENT; EMBRYO; EMBRYO CULTURE; EPIDERMIS; GASTRULA; GENE EXPRESSION; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; MESODERM; NONHUMAN; STAINING; TISSUE FIXATION; TISSUE SECTION; WILD TYPE; XENOPUS;

ANIMALS; CELL DIFFERENTIATION; CELL LINEAGE; EMBRYO, NONMAMMALIAN; GASTRULA; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; STAINING AND LABELING; XENOPUS; XENOPUS PROTEINS;

EID: 34548763109     PISSN: 00121592     EISSN: 1440169X     Source Type: Journal    
DOI: 10.1111/j.1440-169X.2007.00958.x     Document Type: Article

References (21)

1. Artinger, M., Blitz, I., Inoue, K., Tran, U. Cho, K. W. 1997. Interaction of goosecoid and brachyury in Xenopus mesoderm patterning. Mech. Dev. 65, 187 196.
2. Bauer, D. V., Huang, S. Moody, S. A. 1994. The cleavage stage origin of Spemann's Organizer: analysis of the movements of blastomere clones before and during gastrulation in Xenopus. Development 120, 1179 1189.
3. Brent, A. E., Schweitzer, R. Tabin, C. J. 2003. Somitic compartment of tendon progenitors. Cell. 113, 235 248.
4. Cho, K. W., Blumberg, B., Steinbeisser, H. De Robertis, E. M. 1991. Molecular nature of Spemann's Organizer: the role of the Xenopus homeobox gene goosecoid. Cell 67, 1111 1120.
5. Dale, L. Slack, J. M. W. 1987. Fate map for the 32-cell stage of Xenopus laevis. Development 99, 527 551.
6. Dent, J. A. Klymkowsky, M. W. 1989. Whole-mount analyses of cytoskeletal reorganization and function during oogenesis and early embryogenesis in Xenopus. In The Cell Biology of Ferlization (eds H. Schatten G. Schatten pp. 63 103. Academic Press, New York.
7. Doniach, T. Musci, T. J. 1995. Induction of anteroposterior neural pattern in Xenopus: evidence for a quantitative mechanism. Mech. Dev. 53, 403 413.
8. Gerhart, J. 2001. Evolution of the Organizer and the chordate body plan. Int. J. Dev. Biol. 45, 133 153.
9. Harland, R. M. 1991. In situ hybridization: an improved whole-mount method for Xenopus embryos. Meth Cell Biol. 36, 685 695.
10. Jones, C. M. Smith, J. C. 1998. Establishment of a BMP-4 morphogen gradient by long-range inhibition. Dev. Biol. 194, 12 17.
11. Jowett, T. Lettice, L. 1994. Whole-mount in situ hybridizations on zebrafish embryos using a mixture of digoxigenin- and fluorescein-labelled probes. Trends Genet. 10, 73 74.
12. Kageura, H. 1990. Spatial distribution of the capacity to initiate a secondary embryo in the 32-cell embryo of Xenopus laevis. Dev. Biol. 142, 432 438.
13. Kageura, H. Yamana, K. 1986. Pattern formation in 8-cell composite embryos of Xenopus laevis. J. Embryol. Exp. Morphol. 91, 79 100.
14. Knecht, A. K., Good, P. J., Dawid, I. B. Harland, R. M. 1995. Dorsal-ventral patterning and differentiation of noggin-induced neural tissue in the absence of mesoderm. Development 121, 1927 1936.
15. Koga, M. 1999. Compensation for the lost prospective central nervous system by expansion of the prospective epidermis in the '16-cell' embryos of Xenopus laevis lacking all animal dorsal blastomeres. Zool. Sci. 16, 225 236.
16. Lopez-Sanchez, C., Garcia-Martinez, V., Lawson, A., Chapman, S. Schoenwolf, G. 2004. Rapid triple-labeling method combining in situ hybridization and double immunocytochemistry. Dev. Dyn. 230, 309 315.
17. Mayor, R., Morgan, R. Sargent, M. G. 1995. Induction of the prospective neural crest of Xenopus. Development 121, 767 777.
18. Moody, A. 1987. Fates of the blastomeres of the 32-cell-stage Xenopus embryo. Dev Biol. 122, 300 319.
19. Sive, H. L., Grainger, R. M. Harland, R. M. 2000. Early Development of Xenopus Laevis: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.
20. Smith, J. C., Price, B. M. J., Green, J. B. A., Weigel, D. Herrmann, B. G. 1991. Expression of the Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction. Cell 67, 79 87.
21. Vodicka, M. A. Gerhart, J. C. 1995. Blastomere derivation and domains of gene expression in the Spemann Organizer of Xenopus laevis. Development 121, 3505 3518.