Flavone inhibits vascular contraction by decreasing phosphorylation of the myosin phosphatase target subunit

Clinical and Experimental Pharmacology and Physiology

Volumn 34, Issue 11, 2007, Pages 1116-1120

  Jeon, Su Bun ^a   Kim, Gutae ^a   Kim, Jee In ^a   Seok, Young Mi ^a   Kim, Sang Hyun ^a   Suk, Kyoungho ^a   Shin, Heung Mook ^b   Lee, Young Ho ^c   Kim, In Kyeom ^a  

^a Kyungpook National University School of Medicine   (South Korea)

^b Dongguk University   (South Korea)

^c Yonsei University College of Medicine   (South Korea)

Author keywords

Flavone; Myosin light chain (MLC20) phosphorylation; MYPT1; U46619; Vasorelaxation

Indexed keywords

15 HYDROXY 11ALPHA,9ALPHA EPOXYMETHANOPROSTA 5,13 DIENOIC ACID; CYCLIC AMP; CYCLIC GMP; FLAVONE; MYOSIN LIGHT CHAIN; MYOSIN PHOSPHATASE; PHOSPHATASE; THROMBOXANE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; BLOOD VESSEL TONE; CALCIUM TRANSPORT; CONTROLLED STUDY; DOSE RESPONSE; DRUG MECHANISM; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; EXTRACELLULAR FLUID; MUSCLE CONTRACTION; NONHUMAN; RAT; SMOOTH MUSCLE; VASCULAR RING; VASCULAR SMOOTH MUSCLE; VASOCONSTRICTION;

15-HYDROXY-11 ALPHA,9 ALPHA-(EPOXYMETHANO)PROSTA-5,13-DIENOIC ACID; ANIMALS; AORTA, THORACIC; DOSE-RESPONSE RELATIONSHIP, DRUG; FLAVONOIDS; MALE; MYOSIN LIGHT CHAINS; PHOSPHORYLATION; PROTEIN PHOSPHATASE 1; RATS; RATS, SPRAGUE-DAWLEY; RHO-ASSOCIATED KINASES; SIGNAL TRANSDUCTION; VASOCONSTRICTOR AGENTS; VASODILATION; VASODILATOR AGENTS;

EID: 34548681860     PISSN: 03051870     EISSN: 14401681     Source Type: Journal    
DOI: 10.1111/j.1440-1681.2007.04672.x     Document Type: Article

References (28)

1. Formica JV, Regelson W. Review of the biology of quercetin and related bioflavonoids. Food Chem. Toxicol. 1995 33 : 1061 80.
2. Middleton Jr. E, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells. Implications for inflammation, heart disease, and cancer. Pharmacol. Rev. 2000 52 : 673 751.
3. Fitzpatrick DF, Hirschfield SL, Coffey RG. Endothelium-dependent vasorelaxing activity of wine and other grape products. Am. J. Physiol. Heart Circ. Physiol. 2000 265 : H774 8.
4. Flesch M, Schwarz A, Bohm M. Effects of red and white wine on endothelium-dependent vasorelaxation of rat aorta and human coronary arteries. Am. J. Physiol. 1998 275 : H1183 90.
5. Ajay M, Gilani AU, Mustafa MR. Effects of flavonoids on vascular smooth muscle of the isolated rat thoracic aorta. Life Sci. 2003 74 : 603 12.
6. Herrera MD, Zarzuelo A, Jimenez J et al. Effects of flavonoids on rat aortic smooth muscle contractility: Structure-activity relationships. Gen. Pharmacol. 1996 27 : 273 7.
7. Chan EC, Pannangpetch P, Woodman OL. Relaxation to flavones and flavonols in rat isolated thoracic aorta. Mechanism of action and structure-activity relationships. J. Cardiovasc. Pharmacol. 2000 35 : 326 33.
8. Duarte J, Perez Vizcaino F, Utrilla P et al. Vasodilatory effects of flavonoids in rat aortic smooth muscle. Structure-activity relationships. Gen. Pharmacol. 1993 24 : 857 62.
9. Ko FN, Huang TF, Teng CM. Vasodilatory action mechanisms of apigenin isolated from Apium graveolens in rat thoracic aorta. Biochim. Biophys. Acta 1991 1115 : 69 74.
10. Horowitz A, Menice CB, Laporte R et al. Mechanisms of smooth muscle contractions. Physiol. Rev. 1996 76 : 976 1003.
11. Ito M, Nakano T, Erdodi F et al. Myosin phosphatase: Structure, regulation and function. Mol. Cell Biochem. 2004 259 : 197 209.
12. 2+ sensitivity of smooth muscle and nonmuscle myosin II: Modulated by G proteins, kinases, and myosin phosphatase. Physiol. Rev. 2003 83 : 1325 58.
13. Muranyi A, Derkach D, Erdodi F et al. Phosphorylation of Thr695 and Thr850 on the myosin phosphatase target subunit: Inhibitory effects and occurrence in A7r5 cells. FEBS Lett. 2005 579 : 6611 15.
14. Ichikawa K, Ito M, Hartshorne DJ. Phosphorylation of the large subunit of myosin phosphatase and inhibition of phosphatase activity. J. Biol. Chem. 1996 271 : 4733 40.
15. Velasco G, Armstrong C, Morrice N et al. Phosphorylation of the regulatory subunit of smooth muscle protein phosphatase 1M at Thr850 induces its dissociation from myosin. FEBS Lett. 2002 527 : 101 4.
16. 2+ sensitization: Rho-associated kinase-mediated phosphorylation of MYPT1 at Thr-855, but not Thr-697. Biochem. J. 2005 389 : 763 74.
17. Jeon SB, Jin F, Kim JI et al. A role for Rho kinase in vascular contraction evoked by sodium fluoride. Biochem. Biophys. Res. Commun. 2006 343 : 27 33.
18. Persechini A, Kamm KE, Stull JT. Different phosphorylated forms of myosin in contracting tracheal smooth muscle. J. Biol. Chem. 1986 261 : 6293 9.
19. Lunte SM. Structural classification of flavonoids in beverages by liquid chromatography with ultraviolet-visible and electrochemical detection. J. Chromatogr. 1987 384 : 371 82.
20. Woodman OL, Meeker WF, Boujaoude M. Vasorelaxant and antioxidant activity of flavonols and flavones: Structure-activity relationships. J. Cardiovasc. Pharmacol. 2005 46 : 302 9.
21. Kimura K, Ito M, Amano M et al. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science 1996 273 : 245 8.
22. Hart MJ, Jiang X, Kozasa T et al. Direct stimulation of the guanine nucleotide exchange activity of p115 RhoGEF by Galpha13. Science 1998 280 : 2112 14.
23. Gohla A, Schultz G, Offermanns S. Role for G(12)/G(13) in agonist-induced vascular smooth muscle cell contraction. Circ. Res. 2000 87 : 221 7.
24. Fukata Y, Amano M, Kaibuchi K. Rho-Rho-kinase pathway in smooth muscle contraction and cytoskeletal reorganization of non-muscle cells. Trends Pharmacol. Sci. 2001 22 : 32 9.
25. Leung T, Manser E, Tan L et al. A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes. J. Biol. Chem. 1995 270 : 29 051 4.
26. Matsui T, Amano M, Yamamoto T et al. Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho. EMBO J. 1996 15 : 2208 16.
27. Kureishi Y, Kobayashi S, Amano M et al. Rho-associated kinase directly induces smooth muscle contraction through myosin light chain phosphorylation. J. Biol. Chem. 1997 272 : 12 257 60.
28. Tsai MH, Jiang MJ. Rho-kinase-mediated regulation of receptor-agonist- stimulated smooth muscle contraction. Pflügers Arch. 2006 453 : 223 32.