Understanding nonaflatoxigenicity of Aspergillus sojae: A windfall of aflatoxin biosynthesis research

Applied Microbiology and Biotechnology

Volumn 76, Issue 5, 2007, Pages 977-984

  Chang, Perng Kuang ^a   Matsushima, Kenichiro ^b   Takahashi, Tadashi ^b   Yu, Jiujiang ^a   Abe, Keietsu ^c   Bhatnagar, Deepak ^a   Yuan, Gwo Fang ^d   Koyama, Yasuji ^b   Cleveland, Thomas E ^a  

^a SOUTHERN REGIONAL RESEARCH CENTER   (United States)

^b Noda Institute for Scientific Research   (Japan)

^c TOHOKU UNIVERSITY   (Japan)

^d FOOD INDUSTRY RESEARCH AND DEVELOPMENT INSTITUTE   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

AFLATOXINS; BIOSYNTHESIS; FERMENTATION; GENES; MUTAGENESIS; TRANSCRIPTION;

ASPERGILLUS PARASITICUS; GENE REGULATION; NONAFLATOXIGENICITY; POLYKETIDE SYNTHASE GENE;

FUNGI;

AFLATOXIN; POLYKETIDE SYNTHASE;

ENZYME; FERMENTATION; FUNGUS; GENE EXPRESSION; TOXIN;

ASPERGILLUS FLAVUS; ASPERGILLUS PARASITICUS; BIOSYNTHESIS; FERMENTATION; GENE CLUSTER; GENE CONTROL; GENE FUNCTION; GENE MUTATION; GENE STRUCTURE; GENETIC TRANSCRIPTION; GENOME ANALYSIS; NONHUMAN; SHORT SURVEY; TOXICITY;

AFLATOXINS; ASPERGILLUS; ASPERGILLUS FLAVUS; DNA-BINDING PROTEINS; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; POINT MUTATION; TRANSCRIPTION FACTORS;

ASPERGILLUS; ASPERGILLUS FLAVUS; ASPERGILLUS PARASITICUS; ASPERGILLUS SOJAE; FUNGI;

EID: 34548674502     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-1116-4     Document Type: Short Survey

References (78)

1. Barros G, Chiotta ML, Torres A, Chulze S (2006) Genetic diversity in Aspergillus parasiticus population from the peanut agroecosystem in Argentina. Lett Appl Microbiol 42:560-566
2. Blumenthal CZ (2004) Production of toxic metabolites in Aspergillus niger, Aspergillus oryzae, and Trichoderma reesei: justification of mycotoxin testing in food grade enzyme preparations derived from the three fungi. Regul Toxicol Pharmacol 39:214-228
3. Bok JW, Balajee SA, Marr KA, Andes D, Nielsen KF, Frisvad JC, Keller NP (2005) LaeA, a regulator of morphogenetic fungal virulence factors. Eukaryot Cell 4:1574-1582
4. Brown MP, Brown-Jenco CS, Payne GA (1999) Genetic and molecular analysis of aflatoxin biosynthesis. Fungal Genet Biol 26:81-98
5. Calvo AM, Bok J, Brooks W, Keller NP (2004) veA is required for toxin and sclerotial production in Aspergillus parasiticus. Appl Environ Microbiol 70:4733-4739
6. Cary JW, Ehrlich KC, Wright M, Chang P-K, Bhatnagar D (2000) Generation of aflR disruption mutants of Aspergillus parasiticus. Appl Microbiol Biotechnol 53:680-684
7. Cary JW, Klich MA, Beltz SB (2005) Characterization of aflatoxin-producing fungi outside of Aspergillus section Flavi. Mycologia 97:425-432
8. Cary JW, Ehrlich KC, Bland JM, Montalbano BG (2006) The aflatoxin biosynthesis cluster gene, aflX, encodes an oxidoreductase involved in conversion of versicolorin A to demethylsterigmatocystin. Appl Environ Microbiol 72:1096-1101
9. Chang P-K (2003) The Aspergillus parasiticus protein AFLJ interacts with the aflatoxin pathway-specific regulator AFLR. Mol Genet Genom 268:711-719
10. Chang P-K (2004) Lack of interaction between AFLR and AFLJ contributes to nonaflatoxigenicity of Aspergillus sojae. J Biotechnol 107:245-253
11. Chang P-K, Cary JW, Bhatnagar D, Cleveland TE, Bennett JW, Linz JE, Woloshuk CP, Payne GA (1993) Cloning of the Aspergillus parasiticus apa-2 gene associated with the regulation of aflatoxin biosynthesis. Appl Environ Microbiol 59:3273-3279
12. Chang P-K, Bhatnagar D, Cleveland TE, Bennett JW (1995a) Sequence variability in homologs of the aflatoxin pathway gene aflR distinguishes species in Aspergillus section Flavi. Appl Environ Microbiol 61:40-43
13. Chang P-K, Ehrlich KC, Yu J, Bhatnagar D, Cleveland TE (1995b) Increased expression of Aspergillus parasiticus aflR, encoding a sequence-specific DNA-binding protein, relieves nitrate inhibition of aflatoxin biosynthesis. Appl Environ Microbiol 61:2372-2377
14. Chang P-K, Yu J, Bhatnagar D, Cleveland TE (1999) The carboxy-terminal portion of the aflatoxin pathway regulatory protein AFLR of Aspergillus parasiticus activates GAL1::lacZ gene expression in Saccharomyces cerevisiae. Appl Environ Microbiol 65:2508-2512
15. Chang P-K, Bennett JW, Cotty PJ (2002) Association of aflatoxin biosynthesis and sclerotial development in Aspergillus parasiticus. Mycopathologia 153:41-48
16. Chang P-K, Yu J, Yu JH (2004) aflT, a MFS transporter-encoding gene located in the aflatoxin gene cluster, does not have a significant role in aflatoxin secretion. Fungal Genet Biol 41:911-920
17. Chang P-K, Horn BW, Dorner JW (2005) Sequence breakpoints in the aflatoxin biosynthesis gene cluster and flanking regions in nonaflatoxigenic Aspergillus flavus isolates. Fungal Genet Biol 42:914-923
18. Chang P-K, Ehrlich KC, Hua SS (2006) Cladal relatedness among Aspergillus oryzae isolates and Aspergillus flavus S and L morphotype isolates. Int J Food Microbiol 108:172-177
19. Crawford JM, Dancy BC, Hill EA, Udwary DW, Townsend CA (2006) Identification of a starter unit acyl-carrier protein transacylase domain in an iterative type I polyketide synthase. Proc Natl Acad Sci U S A 103:16728-16733
20. Ehrlich KC, Montalbano BG, Cary JW (1999) Binding of the C6-zinc cluster protein, AFLR, to the promoters of aflatoxin pathway biosynthesis genes in Aspergillus parasiticus. Gene 230:249-257
21. Ehrlich KC, Chang P-K, Yu J, Cotty PJ (2004) Aflatoxin biosynthesis cluster gene cypA is required for G aflatoxin formation. Appl Environ Microbiol 70:6518-6524
22. Ehrlich KC, Montalbano B, Boue SM, Bhatnagar D (2005a) An aflatoxin biosynthesis cluster gene encodes a novel oxidase required for conversion of versicolorin A to sterigmatocystin. Appl Environ Microbiol 71:8963-8965
23. Ehrlich KC, Yu J, Cotty PJ (2005b) Aflatoxin biosynthesis gene clusters and flanking regions. J Appl Microbiol 99:518-527
24. Flaherty JE, Payne GA (1997) Overexpression of aflR leads to upregulation of pathway gene transcription and increased aflatoxin production in Aspergillus flavus. Appl Environ Microbiol 63:3995-4000
25. 1, sterigmatocystin and 3-O-methylsterigmatocystin, Aspergillus rambellii sp. nov. Syst Appl Microbiol 28:442-453
26. Geiser DM, Pitt JI, Taylor JW (1998) Cryptic speciation and recombination in the aflatoxin-producing fungus Aspergillus flavus. Proc Natl Acad Sci U S A 95:388-393
27. Geiser DM, Dorner JW, Horn BW, Taylor JW (2000) The phylogenetics of mycotoxin and sclerotium production in Aspergillus flavus and Aspergillus oryzae. Fungal Genet Biol 31:169-179
28. Goto T, Wicklow DT, Ito Y (1996) Aflatoxin and cyclopiazonic acid production by a sclerotium-producing Aspergillus tamarii strain. Appl Environ Microbiol 62:4036-4038
29. α protein-dependent signaling pathway. EMBO J 16:4916-4923
30. Horn BW, Greene RL, Sobolev VS, Dorner JW, Powell JH (1996) Association of morphology and mycotoxin production with vegetative compatibility groups in Aspergillus flavus, A. parasiticus, and A. tamarii. Mycologia 88:574-587
31. Ito Y, Peterson SW, Wicklow DT, Goto T (2001) Aspergillus pseudotamarii, a new aflatoxin producing species in Aspergillus section Flavi. Mycol Res 105:233-239
32. Klich MA (2002) Identification of common Aspergillus species. Centraalbureau voor Schimmelcultures, The Netherlands
33. Klich MA, Mullaney EJ (1987) DNA restriction enzyme fragment polymorphism as a tool for rapid differentiation of Aspergillus flavus from Aspergillus oryzae. Exp Mycol 1:170-175
34. Klich MA, Mullaney EJ (1989) Use of bleomycin-containing medium to distinguish Aspergillus parasiticus from A. sojae. Mycologia 81:159-160
35. Klich MA, Pitt JI (1988) A laboratory guide to common Aspergillus species and their teleomorphs. CSIRO Division of Food Processing, North Ryde, NSW
36. Klich MA, Yu J, Chang P-K, Mullaney EJ, Bhatnagar D, Cleveland TE (1995) Hybridization of genes involved in aflatoxin biosynthesis to DNA of aflatoxigenic and non-aflatoxigenic aspergilli. Appl Microbiol Biotechnol 44:439-443
37. Klich MA, Montalbano B, Ehrlich KE (1997) Northern analysis of aflatoxin biosynthesis genes in Aspergillus parasiticus and Aspergillus sojae. Appl Microbiol Biotechnol 47:246-249
38. Kumeda Y, Asao T (1996) Single-strand conformation polymorphism analysis of PCR-amplified ribosomal DNA internal transcribed spacers to differentiate species of Aspergillus section Flavi. Appl Environ Microbiol 62:2947-2952
39. Kumeda Y, Asao T (2001) Heteroduplex panel analysis, a novel method for genetic identification of Aspergillus section Flavi strains. Appl Environ Microbiol 67:4084-4090
40. Kurtzman CP, Smiley MJ, Robert CJ, Wicklow DT (1986) DNA relatedness among wild and domesticated species in the Aspergillus flavus group. Mycologia 78:955-959
41. Lee CZ, Liou GY, Yuan GF (2006a) Comparison of the aflR gene sequences of strains in Aspergillus section Flavi. Microbiology 152:161-170
42. Lee YH, Tominaga M, Hayashi R, Sakamoto K, Yamada O, Akita O (2006b) Aspergillus oryzae strains with a large deletion of the aflatoxin biosynthetic homologous gene cluster differentiated by chromosomal breakage. Appl Microbiol Biotechnol 72:339-345
43. Matsushima K, Chang P-K, Yu J, Abe K, Bhatnagar D, Cleveland TE (2001a) Pre-termination in aflR of Aspergillus sojae inhibits aflatoxin biosynthesis. Appl Microbiol Biotechnol 55:585-589
44. Matsushima K, Yashiro K, Hanya Y, Abe K, Yabe K, Hamasaki T (2001b) Absence of aflatoxin biosynthesis in koji mold (Aspergillus sojae). Appl Microbiol Biotechnol 55:771-776
45. Meyers DM, O'Brian G, Du WL, Bhatnagar D, Payne GA (1998) Characterization of aflJ, a gene required for conversion of pathway intermediates to aflatoxin. Appl Environ Microbiol 64:3713-3717
46. Minto RE, Townsend CA (1997) Enzymology and molecular biology of aflatoxin biosynthesis. Chem Rev 97:537-2555
47. Montiel D, Dickinson MJ, Lee HA, Dyer PS, Jeenes DJ, Roberts IN, James S, Fuller LJ, Matsuchima K, Archer DB (2003) Genetic differentiation of the Aspergillus section Flavi complex using AFLP fingerprints. Mycol Res 107:1427-1434
48. Moody SF, Tyler BM (1990a) Restriction enzyme analysis of mitochondrial DNA of the Aspergillus flavus group: A. flavus, A. parasiticus, and A. nomius. Appl Environ Microbiol 56:2441-2452
49. Moody SF, Tyler BM (1990b) Use of nuclear DNA restriction fragment length polymorphisms to analyze the diversity of the Aspergillus flavus group: A. flavus, A. parasiticus, and A. nomius. Appl Environ Microbiol 56:2453-2461
50. Otsuki T, Wilson JS, Sewadeh M (2001) What price precaution? European harmonization of aflatoxin regulations and African groundnuts exports. Euro Rev Agri Econom 28:263-284
51. Pariza MW, Johnson EA (2001) Evaluating the safety of microbial enzyme preparations used in food processing: update for a new century. Regul Toxicol Pharmacol 33:173-186
52. Payne GA, Nystrom GJ, Bhatnagar D, Cleveland TE, Woloshuk CP (1993) Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus. Appl Environ Microbiol 59:156-162
53. Payne GA, Nierman WC, Wortman JR, Pritchard BL, Brown D, Dean RA, Bhatnagar D, Cleveland TE, Machida M, Yu J (2006) Whole genome comparison of Aspergillus flavus and A. oryzae. Med Mycol 44(Suppl):9-11
54. Price MS, Yu J, Nierman WC, Kim HS, Pritchard B, Jacobus CA, Bhatnagar D, Cleveland TE, Payne GA (2006) The aflatoxin pathway regulator AflR induces gene transcription inside and outside of the aflatoxin biosynthetic cluster. FEMS Microbiol Lett 255:275-279
55. Roze LV, Beaudry RM, Keller NP, Linz JE (2004) Regulation of aflatoxin synthesis by FadA/cAMP/protein kinase A signaling in Aspergillus parasiticus. Mycopathologia 158:219-232
56. Sakuno E, Wen Y, Hatabayashi H, Arai H, Aoki C, Yabe K, Nakajima H (2005) Aspergillus parasiticus cyclase catalyzes two dehydration steps in aflatoxin biosynthesis. Appl Environ Microbiol 71:2999-3006
57. Schaffer AA, Aravind L, Madden TL, Shavirin S, Spouge JL, Wolf YI, Koonin EV, Altschul SF (2001) Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements. Nucleic Acids Res 29:2994-3005
58. Schechtman MG (1992) United States government regulations affecting aspergilli and their products. Biotechnology 23:271-296
59. Sekine H, Nasuno S, Iguchi N (1969) Isolation of highly proteolytic mutants from Aspergillus sojae. Agri Biol Chem 33:1477-1482
60. Skory CD, Chang P-K, Linz JE (1993) Regulated expression of the nor-1 and ver-1 genes associated with aflatoxin biosynthesis. Appl Environ Microbiol 59:1642-1646
61. Takahashi T, Chang P-K, Matsushima K, Yu J, Abe K, Bhatnagar D, Cleveland TE, Koyama Y (2002) Nonfunctionality of Aspergillus sojae aflR in a strain of Aspergillus parasiticus with a disrupted aflR gene. Appl Environ Microbiol 68:3737-3743
62. Tominaga M, Lee YH, Hayashi R, Suzuki Y, Yamada O, Sakamoto K, Gotoh K, Akita O (2006) Molecular analysis of an inactive aflatoxin biosynthesis gene cluster in Aspergillus oryzae RIB strains. Appl Environ Microbiol 72:484-490
63. Trail F, Chang P-K, Cary J, Linz JE (1994) Structural and functional analysis of the nor-1 gene involved in the biosynthesis of aflatoxins by Aspergillus parasiticus. Appl Environ Microbiol 60:4078-4085
64. Trail F, Mahanti N, Linz J (1995) Molecular biology of aflatoxin biosynthesis. Microbiology 141:755-765
65. Vaamonde G, Patriarca A, Fernandez Pinto V, Comerio R, Degrossi C (2003) Variability of aflatoxin and cyclopiazonic acid production by Aspergillus section Flavi from different substrates in Argentina. Int J Food Microbiol 88:79-84
66. van Dijck PW, Selten GC, Hempenius RA (2003) On the safety of a new generation of DSM Aspergillus niger enzyme production strains. Regul Toxicol Pharmacol 38:27-35
67. Wang L, Yokoyama K, Takahasi H, Kase N, Hanya Y, Yashiro K, Miyaji M, Nishimura K (2001) Identification of species in Aspergillus section Flavi based on sequencing of the mitochondrial cytochrome b gene. Int J Food Microbiol 71:75-86
68. Watson AJ, Fuller LJ, Jeenes DJ, Archer DB (1999) Homologs of aflatoxin biosynthesis genes and sequence of aflR in Aspergillus oryzae and Aspergillus sojae. Appl Environ Microbiol 65:307-310
69. Wei DL, Jong SC (1986) Production of aflatoxins by strains of the Aspergillus flavus group maintained in ATCC. Mycopathologia 93:19-24
70. Wen Y, Hatabayashi H, Arai H, Kitamoto HK, Yabe K (2005) Function of the cypX and moxY genes in aflatoxin biosynthesis in Aspergillus parasiticus. Appl Environ Microbiol 71:3192-3198
71. Wicklow DT (1984) Conidium germination rate in wild and domesticated yellow-green Aspergilli. Appl Environ Microbiol 47:299-300
72. Wilkinson JR, Yu J, Bland JM, Nierman WC, Bhatnagar D, Cleveland TE (2007) Amino acid supplementation reveals differential regulation of aflatoxin biosynthesis in Aspergillus flavus NRRL 3357 and Aspergillus parasiticus SRRC 143. Appl Microbiol Biotechnol 74:1308-1319
73. Yabe K, Nakajima H (2004) Enzyme reactions and genes in aflatoxin biosynthesis. Appl Microbiol Biotechnol 64:745-755
74. Yu J, Chang P-K, Cary JW, Wright M, Bhatnagar D, Cleveland TE, Payne GA, Linz JE (1995) Comparative mapping of aflatoxin pathway gene clusters in Aspergillus parasiticus and Aspergillus flavus. Appl Environ Microbiol 61:2365-2371
75. Yu J, Woloshuk CP, Bhatnagar D, Cleveland TE (2000) Cloning and characterization of avfA and omtB genes involved in aflatoxin biosynthesis in three Aspergillus species. Gene 248:157-167
76. Yu J, Chang P-K, Ehrlich KC, Cary JW, Bhatnagar D, Cleveland TE, Payne GA, Linz JE, Woloshuk CP, Bennett JW (2004a) Clustered pathway genes in aflatoxin biosynthesis. Appl Environ Microbiol 70:1253-1262
77. Yu J, Whitelaw CA, Nierman WC, Bhatnagar D, Cleveland TE (2004b) Aspergillus flavus expressed sequence tags for identification of genes with putative roles in aflatoxin contamination of crops. FEMS Microbiol Lett 237:333-340
78. Yuan GF, Liu CS, Chen CC (1995) Differentiation of Aspergillus parasiticus from Aspergillus sojae by random amplification of polymorphic DNA. Appl Environ Microbiol 61:2384-2387