Mass spectrometric identification of pyroglutamic acid in peptides following selective hydrolysis

Analytical Biochemistry

Volumn 370, Issue 1, 2007, Pages 118-120

  Mandal, Amit Kumar ^a   Balaram, Padmanabhan ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

MASS SPECTROMETRIC IDENTIFICATION; PYROGLUTAMIC ACIDS; SELECTIVE HYDROLYSIS;

MASS SPECTROMETRY;

CARBOXYLIC ACID; CONOTOXIN; PEPTIDE; PYROGLUTAMIC ACID;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; DISSOCIATION; ESTERIFICATION; FRACTIONATION; HYDROLYSIS; MASS SPECTROMETER; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY; PRIORITY JOURNAL; REACTION ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 34548621358     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.06.027     Document Type: Article

References (6)

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2. Blomback B. Derivatives of glutamine in peptides. Methods Enzymol. 11 (1967) 398-411
3. Rehder D.S., Dillon T.M., Pipes G.D., and Bondarenko P.V. Reversed phase liquid chromatography/mass spectrometry analysis of reduced monoclonal antibodies in pharmaceutics. J. Chromatogr. A 1102 (2006) 164-175
4. Chelius D., Jing K., Lueras A., Rehder D.S., Dillon T.M., Vizel A., Rajan R.S., Li T., Treuheit M.J., and Bondarenko P.V. Formation of pyroglutamic acid from N-terminal glutamic acid in immunoglobulin gamma antibodies. Anal. Chem. 78 (2006) 2370-2376
5. Craig A.G., Jimenez E.G., Dykert J., Nielsen D.B., Gulyas J., Abogadie F.C., Porter J., Rivier J.E., Cruz L.J., Olivera B.M., and McIntosh J.M. A novel post-translational modification involving bromination of tryptophan: identification of the residue, l-6-bromotryptophan, in peptides from Conus imperials and Conus radiatus venom. J. Biol. Chem. 272 (1997) 4689-4698
6. A.K. Mandal, M.S. Ramasamy, V. Sabareesh, M.E. Openshaw, K.S. Krishnan, P. Balaram, Sequencing of T-superfamily conotoxins from Conus virgo: pyroglutamic acid identification and disulfide arrangement by MALDI mass spectrometry, J. Am. Soc. Mass Spectrom., accepted for publication, doi:10.1016/j.jasms.2007.04.099.