Analysis of secreted protein profile and enzymatic activities from Corynebacterium diphtheriae and Bordetella pertussis on production batch media using peptide quenched fluorescent substrates

Preparative Biochemistry and Biotechnology

Volumn 37, Issue 4, 2007, Pages 353-367

  Perpetuo, Elen A ^a   Lebrun, Ivo ^a   Juliano, Luis ^b   Juliano, Maria Aparecida ^b   Sakauchi, Maria Aparecida ^a   Prado, Sally M A ^a  

^a INSTITUTO BUTANTAN   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Culture media; Diphtheria toxin; Fluorescent substrates; Pertussis toxin; Proteolytic activity

Indexed keywords

BACTERIAL TOXIN; BUFFER; FLUORESCENT DYE; PEPTIDE; PEPTIDE HYDROLASE; PERTUSSIS TOXIN; SODIUM CHLORIDE; TROMETAMOL;

AMINO ACID SEQUENCE; ARTICLE; BORDETELLA PERTUSSIS; CHEMISTRY; CORYNEBACTERIUM DIPHTHERIAE; CULTURE MEDIUM; ENZYME SPECIFICITY; ENZYMOLOGY; FILTRATION; GEL CHROMATOGRAPHY; GROWTH, DEVELOPMENT AND AGING; HYDROLYSIS; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS;

AMINO ACID SEQUENCE; BACTERIAL TOXINS; BORDETELLA PERTUSSIS; BUFFERS; CHROMATOGRAPHY, GEL; CORYNEBACTERIUM DIPHTHERIAE; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FILTRATION; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PEPTIDE HYDROLASES; PEPTIDES; PERTUSSIS TOXIN; SODIUM CHLORIDE; SUBSTRATE SPECIFICITY; TROMETHAMINE;

BACTERIA (MICROORGANISMS); BORDETELLA PERTUSSIS; CORYNEBACTERIUM DIPHTHERIAE;

EID: 34548594118     PISSN: 10826068     EISSN: 15322297     Source Type: Journal    
DOI: 10.1080/10826060701593274     Document Type: Article

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