Protocol for the fabrication of enzymatically crosslinked gelatin microchannels for microfluidic cell culture

Nature Protocols

Volumn 2, Issue 7, 2007, Pages 1782-1788

  Paguirigan, Amy L ^a   Beebe, David J ^a  

^a University of Wisconsin Madison   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CROSS LINKING REAGENT; ENZYME; GELATIN; GLUTAMINE;

ARTICLE; CELL FUNCTION; CHEMISTRY; CULTURE TECHNIQUE; EXTRACELLULAR MATRIX; GEL; GENE EXPRESSION; GENETICS; METHODOLOGY; MICROFLUIDIC ANALYSIS; MICROFLUIDICS; PHYSIOLOGY;

CELL CULTURE TECHNIQUES; CELL PHYSIOLOGY; CROSS-LINKING REAGENTS; ENZYMES; EXTRACELLULAR MATRIX; GELATIN; GELS; GENE EXPRESSION; GLUTAMINE; MICROFLUIDIC ANALYTICAL TECHNIQUES; MICROFLUIDICS;

EID: 34548562486     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.256     Document Type: Article

References (19)

1. Paguirigan, A. and Beebe, D.J.: Gelatin based microfluidic devices for cell culture. Lab on a Chip 6, 407-413 (2006).
2. Wozniak, M.A.: ROCK-generated contractility regulates breast epithelial cell differentiation in response to the physical properties of a three-dimensional collagen matrix. J. Cell Biol. 163, 583-595 (2003).
3. Atencia, J. and Beebe, D.: Controlled microfluidic interfaces. Nature 437, 648-655 (2005).
4. Walker, G., Mensing, G. and Beebe, D.: Physics and applications of microfluidics in biology. Annu. Rev. Biomed. Eng. 4, 261-286 (2002).
5. Walker, G., Zeringue, H. and Beebe, D.: Microenvironment design considerations for cellular scale studies. Lab on a Chip 4, 91-97 (2004).
6. Condon, M.S.: The role ofthe stromal microenvironment in prostate cancer. Semin. Cancer Biol. 15, 132 (2005).
7. Danen, E.H.J. and Sonnenberg, A.: Integrins in regulation of tissue developmentand function. J. Pathol. 200, 471-480 (2003).
8. Dusseiller, M.R.: An inverted microcontact printing method on topographically structured polystyrene chips for arrayed micro-3-D culturing of single cells. Biomaterials 26, 5917-5925 (2005).
9. Tan, W. and Desai, T.A.: Microfluidic patterning of cellular biopolymer matrices for biomimetic 3-D structures. Biomed. Microdev. 5, 235-244 (2003).
10. Ito, A.: Transglutaminase-mediated gelatin matrices incorporating cell adhesion factors as a biomaterial for tissue engineering. J. Biosci. Bioeng. 95, 196-199 (2003).
11. McDermott, M.K.: Mechanical properties ofbiomimetictissue adhesive based on the microbial transglutaminase-catalyzed crosslinking of gelatin. Biomacromotecutes 5, 1270-1279 (2004).
12. Lim, L., Mine, Y. and Tung, M.: Barrier and tensile properties of transglutaminase cross-linked gelatin films as affected by relative humidity, temperature and glycerol content. J. FoodSci. 64, 616-622 (1999).
13. Mizuno, A., Mitsuiki, M. and Motoki, M.: Effect of transglutaminase treatment on the glass transition of soy protein. J. Agric. Food Chem. 48, 3286-3291 (2000).
14. Chen, R.N., Ho, H.O. and Sheu, M.T.: Characterization of collagen matrices crosslinked using microbial transglutaminase. Biomateriats 26, 4229-4235 (2005).
15. Chen, T.H.: Enzymatic methods for in situ cell entrapment and cell release. Biomacromotecutes 4, 1558-1563 (2003).
16. Aeschlimann, D. and Thomazy, V.: Protein crosslinking in assembly and remodelling of extracellular matrices: the role of transglutaminases. Connect. Tissue Res. 41, 1-27 (2000).
17. Belkin, A.M.: Matrix-dependent proteolysis of surface transglutaminase by membrane-type metalloproteinase regulates cancer cell adhesion and locomotion. J. Biot. Chem. 276, 18415-18422 (2001).
18. Priglinger, S.G.: TGF-beta 2-induced cell surface tissue transglutaminase increases adhesion and migration of RPE cells on fibronectin through the gelatin-binding domain. Invest. Ophthatmot. Vis. Sci. 45, 955-963 (2004).
19. Tourovskaia, A., FigueroaMasot, X. and Folch, A.: Long-term microfluidic cultures of myotube microarrays for high-throughput focal stimulation. Nat. Protoc. 1, 1092-1104 (2006).