Labeling of Bifidobacterium longum cells with 13C-substituted leucine for quantitative proteomic analyses

Applied and Environmental Microbiology

Volumn 73, Issue 17, 2007, Pages 5653-5656

  Couté, Yohann ^a   Hernandez, Céline ^b   Appel, Ron D ^b   Sanchez, Jean Charles ^a   Margolles, Abelardo ^a,c  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b SWISS INSTITUTE OF BIOINFORMATICS   (Switzerland)

^c DEPARTMENT OF MICROBIOLOGY AND BIOCHEMISTRY OF DAIRY PRODUCTS   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CELL CULTURE; CELLS; ISOTOPES; PROTEINS;

BIFIDOBACTERIUM LONGUM CELLS; LEUCINE; PROTEOMIC STRATEGY;

BACTERIA;

CARBON 13; GLUCOSE 6 PHOSPHATE ISOMERASE; GLUTAMATE AMMONIA LIGASE; INOSINATE DEHYDROGENASE; LEUCINE; PYRUVATE KINASE;

AMINO ACID; BACTERIUM; CARBON ISOTOPE; GENE EXPRESSION; PROTEOMICS;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BIFIDOBACTERIUM LONGUM; CULTURE MEDIUM; ISOTOPE LABELING; NONHUMAN; PROTEIN EXPRESSION; PROTEOMICS; QUANTITATIVE ANALYSIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BACTERIOLOGICAL TECHNIQUES; BIFIDOBACTERIUM; CARBON ISOTOPES; CULTURE MEDIA; ISOTOPE LABELING; LEUCINE; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEOME; PROTEOMICS;

BIFIDOBACTERIUM LONGUM;

EID: 34548481968     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00667-07     Document Type: Article

References (19)

1. Bienvenut, W. V., C. Deon, C. Pasquarello, J. M. Campbell, J. C. Sanchez, M. L. Vestal, and D. F. Hochstrasser. 2002. Matrix-assisted laser desorption/ionization-tandem mass spectrometry with high resolution and sensitivity for identification and characterization of proteins. Proteomics 2:868-876.
2. De Man, J. C., M. Rogosa, and M. E. Sharpe. 1960. A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23:130-135.
3. Harmsen, H. J., A. C. Wildeboer-Veloo, G. C. Raangs, A. A. Wagendorp, L. Klijn, J. G. Bindels, and G. W. Welling. 2000. Analysis of intestinal flora development in breast-fed and formula-fed infants by using molecular identification and detection methods. J. Pediatr. Gastroenterol. Nutr. 30:61-67.
4. Laurel, R., F. Morel-Deville, F. Berthier, M. Champomier- Vergès, P. Postma, S. D. Ehrlich, and M. Zagorec. 1996. Carbohydrate utilization in Lactobacillus sake. Appl. Environ. Microbiol. 62:1922-1927.
5. Mann, M. 2006. Functional and quantitative proteomics using SILAC. Nat. Rev. Mol. Cell Biol. 7:952-958.
6. Marco, M. L., S. Pavan, and M. Kleerebezem. 2006. Towards understanding molecular modes of probiotic action. Curr. Opin. Biotechnol. 17:204-210.
7. Neher, S. B., J. Villen, E. C. Oakes, C. E. Bakalarski, R. T. Sauer, S. P. Gygi, and T. A. Baker. 2006. Proteomic profiling of ClpXP substrates after DNA damage reveals extensive instability within SOS regulon. Mol. Cell 22:193-204.
8. Noriega, L., M. Gueimonde, B. Sánchez, A. Margolles, and C. G. de los Reyes-Gavilán. 2004. Effect of the adaptation to high bile salts concentrations on glycosidic activity, survival at low PH and cross-resistance to bile salts in Bifidobacterium. Int. J. Food Microbiol. 94:79-86.
9. Ong, S. E., B. Blagoev, I. Kratchmarova, D. B. Kristensen, H. Steen, A. Pandey, and M. Mann. 2002. Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol. Cell. Proteomics 1:376-386.
10. O'Riordan, K., and G. F. Fitzgerald. 1998. Evaluation of bifidobacteria for the production of antimicrobial compounds and assessment of performance in cottage cheese at refrigeration temperature. J. Appl. Microbiol. 85:103-114.
11. Rogosa, M., L. G. Franklin, and K. D. Perry. 1961. Correlation of the vitamin requirements with cultural and biochemical characters of Lactobacillus spp. J. Gen. Microbiol. 25:473-482.
12. Salminen, S., and M. Gueimonde. 2004. Human studies on probiotics: what is scientifically proven? J. Food Sci. 69:137-140.
13. Sánchez, B., M.-C. Champomier-Verges, P. Anglade, F. Baraige, C. G. de los Reyes-Gavilán, A. Margolles, and M. Zagorec. 2005. Proteomic analysis of global changes in protein expression during bile salt exposure of Bifidobacterium longum NCIMB 8809. J. Bacteriol. 187:5799-5808.
14. 35S]methionine labelling and two-dimensional gel electrophoresis. FEMS Microbiol. Lett. 248:207-215.
15. Schell, M. A., M. Karmirantzou, B. Snel, D. Vilanova, B. Berger, G. Pessi, M. C. Zwahlen, F. Desiere, P. Bork, M. Delley, P. D. Pridmore, and F. Arigoni. 2002. The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract. Proc. Natl. Acad. Sci. USA 99:14422-14427.
16. Scherl, A., Y. Coute, C. Deon, A. Calle, K. Kindbeiter, J.-C. Sanchez, A. Greco, D. Hochstrasser, and J. J. Diaz. 2002. Functional proteomic analysis of human nucleolus. Mol. Biol. Cell 13:4100-4109.
17. Ventura, M., C. Canchaya, G. F. Fitzgerald, R. S. Gupta, and D. van Sinderen. 2007. Genomics as a means to understand bacterial phytogeny and ecological adaptation: the case of bifidobacteria. Antonie Leeuwenhoek 91:351-372.
18. Vitali, B., V. Wasinger, P. Brigidi, and M. Guilhaus. 2005. A proteomic view of Bifidobacterium infantis generated by multi-dimensional chromatography coupled with tandem mass spectrometry. Proteomics 5:1859-1867.
19. Yuan, J., L. Zhu, X. Liu, T. Li, Y. Zhang, T. Ying, B. Wang, J. Wang, H. Dong, E. Feng, Q. Li, J. Wang, H. Wang, K. Wei, X. Zhang, C. Huang, P. Huang, L. Huang, Z. Ming, and H. Wang. 2006. A proteome reference map and proteomics analysis of Bifidobacterium longum NCC2705. Mol. Cell. Proteomics 5:1105-1118.